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171.
Dr. Daisuke Fujiwara Kousuke Mihara Ryo Takayama Yusuke Nakamura Prof. Mitsuhiro Ueda Prof. Takeshi Tsumuraya Prof. Ikuo Fujii 《Chembiochem : a European journal of chemical biology》2021,22(24):3406-3409
Conformationally constrained peptides hold promise as molecular tools in chemical biology and as a new modality in drug discovery. The construction and screening of a target-focused library could be a promising approach for the generation of de novo ligands or inhibitors against target proteins. Here, we have prepared a protein kinase-focused library by chemically modifying helix-loop-helix (HLH) peptides displayed on phage and subsequently tethered to adenosine. The library was screened against aurora kinase A (AurA). The selected HLH peptide Bip - 3 retained the α-helical structure and bound to AurA with a KD value of 13.7 μM. Bip - 3 and the adenosine-tethered peptide Bip - 3 - Adc provided IC50 values of 103 μM and 7.7 μM, respectively, suggesting that Bip - 3 - Adc bivalently inhibited AurA. In addition, the selectivity of Bip - 3 - Adc to several protein kinases was tested, and was highest against AurA. These results demonstrate that chemical modification can enable the construction of a kinase-focused library of phage-displayed HLH peptides. 相似文献
172.
We propose a new geometric method of IR factorization in sector decomposition. The problem is converted into a set of problems in convex geometry. The latter problems are solved using algorithms in combinatorial geometry. This method provides a deterministic algorithm and never falls into an infinite loop. The number of resulting sectors depends on the algorithm of triangulation. Our test implementation shows smaller number of sectors comparing with other existing methods with iterations. 相似文献
173.
K. Friedrich E. Ueda H. Kamo M. Evstatiev B. Krasteva S. Fakirov 《Journal of Materials Science》2002,37(20):4299-4305
Microfibrillar reinforced composites (MFC) comprising an isotropic matrix from a lower melting polymer, i.e., low density polyethylene (LDPE), reinforced by microfibrils of a higher melting polymer, recycled from bottles, i.e., poly(ethylene terephthalate) (PET), were processed under industrially relevant conditions via injection molding in a weight ratio of PET/LDPE = 50/50. Dog bone samples with MFC structure were characterized by means of scanning (SEM) and transmission (TEM) electron microscopy. SEM observations on cryogenic fracture surfaces show an isotropic LDPE matrix reinforced by more or less randomly distributed PET microfibrils. By means of TEM on stained ultrathin slices one observes the formation of transcrystalline layers of LDPE matrix on the surface of the PET microfibrils. In these layers the crystalline lamellae are aligned parallel to each other and are placed perpendicularly to the fibril surfaces. This is in contrast to the bulk matrix where the lamellae are quasi-randomly arranged. 相似文献
174.
Since diamonds indicated low or negative electron affinity, they have been expected to be high performance electron emitters. New diamond electron sources have been developed, which are configured for electron beam instruments such as electron beam lithography systems and electron microscopes. As a result of thermionic emission evaluations, an electron emission current of practical level (116 µA at 600 °C) was obtained. The energy spread of an electron beam generated by thermionic emission was measured as 0.23 eV (FWHM), the value of which was lower than those of a lanthanum hexaborate (LaB6) cathode and a zirconium oxide/tungsten (ZrO/W) cathode measured together. The result of field emission evaluations, a practical beam current (459 pA) and stability (6% rms for 10 h) for scanning electron microscope (SEM) was achieved. The energy spread of a high-current electron beam of over 200 pA generated by field emission from a diamond emitter reached about the same as that of a cold field emission cathode made of tungsten. A scanning electron micrograph (SEM) with a diamond electron source having a tip of submicron order allowed measurement a resolution of 5 nm. It was found that unprecedentedly high-current and high-convergence electron sources could be generated by using diamonds. 相似文献
175.
Phase relations for the Al2O3-CaF2-SiO2 system were investigated from 1673 to 1723 K. The hotfilament technique was applied to observe a two-liquid region and liquidus
for the ternary system. The liquidus saturated with SiO2 was investigated at 1673 K by using the hot-filament technique and the chemical equilibrium technique. In this system, the
addition of Al2O3 to the CaF2-SiO2 system reduces the congruent temperature. A small substitution of Al2O3 for CaF2 increases the solubility of SiO2, namely, if the region of liquid phase could be enlarged. These results suggest that Al2O3 would be an effective substitute of CaF2 in slag for steelmaking. 相似文献
176.
Haruyoshi Otobe Mitsuo Akabori Kazuo Minato 《Journal of the American Ceramic Society》2008,91(6):1981-1985
The oxygen potentials of AmO2− x were measured in the x range of 0.01–0.5 and the temperature range of 1000–1333 K by the electromotive force method. The oxygen potentials at 1333 K were −19.83 kJ/mol for x =0.019 and −319.1 kJ/mol for x =0.485, which were higher than those of CeO2− x by approximately 200 kJ/mol for the corresponding x values. From the dependence of the oxygen potentials on x and temperature, a tentative phase diagram of Am–O system was proposed, which suggested the presence of the intermediate phases of Am7 O12 and Am9 O16 in the Am–O system. 相似文献
177.
Hasegawa Tatsuro Yamauchi Hisashi Sashida Yoshio Matsumoto Mitsuo 《Industrial Electronics, IEEE Transactions on》1985,(3):253-260
Recent application of distributed control systems to large-and medium-scale industrial plants requires effective and high-speed communication among the control devices each other to ensure high-performance operation. 相似文献
178.
Takase F. Tominaga M. Ueda Y. Temma T. Genji T. Oku K. Hira T. Ashizawa A. 《Energy Conversion, IEEE Transaction on》1997,12(2):143-150
This paper deals with a harmonic compensation method using a synchronous machine with resonant field circuits. Harmonics have become one of the major problems in power systems and a lot of methods of harmonic suppression have been studied. The authors have proposed a new method of harmonic suppression using a synchronous machine excited with the 6th harmonic current. This paper shows that armature inductances for the 5th or 7th harmonics decrease when the field circuits are connected to capacitors resonating at the 6th harmonic. The variations of armature impedances are explained theoretically by Park's equations. The optimal capacitances are selected so as to resonate with field inductances for the short-circuited armature. The experiments to suppress the 5th and 7th harmonics using the synchronous machine are successful 相似文献
179.
Design of the Kernel Language for the Parallel Inference Machine 总被引:1,自引:0,他引:1
180.
Yoshiharu Hatakeyama Mitsuo Umetsu Masahiro Minami Satoshi Ohara Seiichi Takami Tadafumi Adschiri 《Journal of Materials Science》2008,43(7):2426-2430
The DNA, which forms a double helical conformation by the highly selective base-pairing rule with the width of 2 nm, is one
of the polymers utilized as a template for nanoparticle assembly. Then, holiday junction structure, where two different DNA
double strands intersect and replace each complement strand, is an important artificial motif for forming two-dimensional
mesh-like DNA morphology. In this study, the two-dimensional sheet with the mesh-like conformation is formed and the growth
of the DNA morphology is controlled by changing the temperature in the formation process. The influence of the cooling procedure
on the formation process of the two-dimensional DNA sheet is observed by atomic force microscope, and the formation of double
strands in the process is traced by absorption spectra. The spectroscopic results demonstrate that the two-dimensional DNA
sheet is strongly dependent on the correlation between the applied cooling process and the melting temperature of double helical
conformation, and that the growth of the two-dimensional DNA sheet can be manipulated by the cooling procedure. Further, we
will show the size control of the DNA sheet by mixing the designed DNA which inhibits the growth of the DNA sheet. 相似文献