Resolution of small molecules by passage through an open capillary

When a solute passes through an open capillary, in which a laminar flow is established, different peak profiles can be obtained according to its diffusion property under a working condition, i.e., the radius and length of the capillary and the flow rate of the carrier solution. If a solute diffuses over the entire cross section of the capillary before it is eluted, a Gaussian-shaped diffusion peak appears, which has an apex at the travel time of the average flow. Insufficient solute diffusion, which is realized, e.g., by increasing flow rates or capillary radius, produces a new peak having an apex at the travel time of the maximum flow. This implies that two solutes can be resolved simply by passing through a capillary. However, our previous study indicated that the diffusion coefficients of two solutes should be at least one order different for their resolution based on this principle, suggesting that its applicability is highly restricted. In the present paper, this concept has been extended to the resolution between dissolved solutes that have similar intrinsic diffusion properties. The incorporation of molecular aggregates in the carrier makes a solute less diffusive according to the extent of their interaction and allows the resolution of a dissolved molecule from other ones differing in the affinity to the molecular aggregates. Several examples of peak resolution for phenols, aromatic hydrocarbons, and inorganic anions are shown and discussed on the basis of the modification of the diffusion natures due to their interactions with micelles or vesicles.     

Weekly doxorubicin in the treatment of patients with AIDS-related Kaposi''s sarcoma. AIDS Clinical Trials Group

It has been shown that the X-ray-sensitive Chinese hamster V79 mutants (V-E5, V-C4 and V-G8) are similar to ataxia-telangiectasia (A-T) cells. To determine whether the AT-like rodent cell mutants are defective in the gene homologous to A-T (group A, C or D), human chromosome 11 was introduced to the V-E5 and V-G8 mutant cells by microcell-mediated chromosome transfer. Forty independent hybrid clones were obtained in which the presence of chromosome 11 was determined by in situ hybridization. The presence of the region of chromosome 11q22-23 was shown by molecular analysis using polymorphic DNA markers specific for the ATA, ATC and ATD loci. Seventeen of the obtained monochromosomal Chinese hamster hybrids contained a cytogenetically normal human chromosome 11, but only twelve hybrid cell lines were shown to contain an intact 11q22-23 region. Despite the complementation of the X-ray sensitivity by a normal chromosome 11 introduced to A-T cells (complementation group D), these twelve Chinese hamster hybrid clones showed lack of complementation of X-ray and streptonigrin hypersensitivity. The observed lack of complementation does not seem to be attributable to hypermethylation of the human chromosome 11 in the rodent cell background, since 5-azacytidine treatment had no effect on the streptonigrin hypersensitivity of the hybrid cell lines. These results indicate that the gene defective in the AT-like rodent cell mutants is not homologous to the ATA, ATC or ATD genes and that the human gene complementing the defect in the AT-like mutants seems not to be located on human chromosome 11.     

Comparative analysis of the determinants of fees charged by fee-based homes for the elderly in urban and suburban areas

This study contributes to establishing the payment function of fee-based homes for the elderly. Disclosure statements from such homes for the elderly in selected urban and suburban cities in Fukuoka Prefecture, Japan—considered a typical prefecture with adjoining urban and suburban areas—were examined. The study compared the determinants of the amounts charged by the homes based on the distinct geographic features of urban and suburban areas. The hedonic price model was combined with a two-stage least squares regression to determine the determinants of lump-sum and monthly payments. We found that the factors that influence monthly payments in urban areas include distance to a park, distance to the coast, meals offered, number of care staff, and initial lump-sum payment. In contrast, factors such as room size, distance to a bus stop, distance to a park, and the number of night staff influence monthly payments in suburban areas. We conclude that the surrounding landscape has a greater influence on monthly payments in urban areas than in suburban areas. Moreover, in suburban areas, the number of night staff has a substantial impact on monthly payments. The results can help provide investment suggestions in certain cases depending on the amount of investment capital.

     

Sarcomere shortening and contraction nodes in stretched-restrained ovine myofibres during post mortem storage

Myofibres of muscles removed from the carcass shorten during rigor mortis. Whether myofibres of stretched and restrained muscle tissues shorten during post mortem storage was examined. Muscle strips removed from the semimembranosus muscle of sheep before rigor onset were stretched and restrained. The muscle strips were fixed separately in formalin solution from 1.5 hr to 12 days post mortem, and embedded in paraffin. Sections were stained with Mallory-azan. Some myofibres in the pre-rigor-excised and stretched-restrained muscle strips had contraction nodes at 1 day post mortem. The contraction node did not increase in frequency hereafter. Two to several successive sarcomeres shortened regularly to form the nodes parallel to the Z-line. In addition, several successive sarcomeres shortened partly and successively from one side to the other side to form the nodes oblique to the Z-line. Oblique and irregular contraction nodes appear to cause kinks or bends of myofibres. Sarcomere lengths were not always uniform in individual myofibres. Formation of contraction nodes and shortening of partial sarcomeres in the stretched-restrained myofibres indicate that the myofibres shorten individually during rigor mortis and vary in shortening states as in muscles left on the carcass.     

Thermal expansion and mechanical properties of pure magnesium containing zirconium tungsten phosphate particles with negative thermal expansion

The magnesium composites containing Zr₂(WO₄)(PO₄)₂ (ZWP) particles with negative thermal expansion (NTE) were synthesized. Dense, unreacted Mg/ZWP composites were fabricated by extruding the two component powders. The coefficient of thermal expansion (CTE) and the mechanical properties of the composites were examined. The CTE of Mg/ZWP composites decreased with an increase in the amount of ZWP in the composites, though the effect was not significant probably because of the low bulk modulus of ZWP. Although there was a tension–compression asymmetry in the room temperature yield strength of Mg without ZWP, the difference reduced in the composites. The decreased asymmetry of yield behavior was suggested to be resulting from the tensile residual stress in the matrix produced during fabrication process because of the NTE of ZWP.     

Proliferation of parathyroid cells negatively correlates with expression of parathyroid hormone-related protein in secondary parathyroid hyperplasia

BACKGROUND: Parathyroid hormone-related protein (PTHrP) is now suspected to act as an autocrine or paracrine regulator of cell growth or differentiation, although it was originally reported as a hypercalcemic substance in malignancies. This study was performed to assess the relationship between PTHrP expression and cell proliferation in human parathyroid glands. METHODS: The localization of PTH and PTHrP was studied in 42 samples of hyperplastic parathyroid from 14 long-term hemodialysis cases with immunohistochemistry and in situ hybridization. Results were compared with proliferative activity (proliferating cell nuclear antigen index: counts of proliferating cell nuclear antigen-positive cells/100 cells). The localization of the PTH/PTHrP receptor was also examined. Ten normal glands were studied as controls. RESULTS: In hyperplasia, cells positive for PTH, PTHrP, or both were observed immunohistochemically. The areas expressing PTHrP mRNA completely coincided with those positive for PTHrP immunohistochemically. Oxyphilic or transitional oxyphilic cells were consistently positive for PTHrP. PTH/PTHrP receptors were located in the cytoplasmic membrane in most parathyroid cells. Proliferating cell nuclear antigen-positive cells were rare in normal glands with an index of 0. 22 +/- 0.09 (mean +/- sem). They were significantly increased in hyperplastic cases but less for PTHrP-positive than for -negative cells (1.25 +/- 0.16 as compared with 7.80 +/- 0.52; P < 0.0001). CONCLUSION: The observed low level of proliferation of PTHrP-positive cells suggests a functional role for PTHrP as a possible growth suppressor in the human parathyroid.     

Bisanthracene‐Based Donor–Acceptor‐type Light‐Emitting Dopants: Highly Efficient Deep‐Blue Emission in Organic Light‐Emitting Devices

Deep‐blue fluorescent compounds are particularly important in organic light‐emitting devices (OLEDs). A donor–accepotor (DA)‐type blue‐emitting compound, 1‐(10‐(4‐methoxyphenyl)anthracen‐9‐yl)‐4‐(10‐(4‐cyanophenyl)anthracen‐9‐yl)benzene ( BD3 ), is synthesized, and for comparison, a nonDA‐type compound, 1,4‐bis(10‐phenylanthracene‐9‐yl)benzene ( BD1 ) and a weak DA‐type compound, 1‐(10‐phenylanthracen‐9‐yl)‐4‐(10‐(4‐cyanophenyl)anthracen‐9‐yl)‐benzene ( BD2 ), are also synthesized. The twisted conformations of the two anthracene units in the compounds, confirmed by single crystal X‐ray analysis, effectively prevent π‐conjugation, and the compound shows deep‐blue photoluminescence (PL) with a high PL quantum efficiency, almost independent of the solvent polarity, resulting from the absence of an intramolecular charge transfer state. The DA‐type molecule BD3 in a non‐doped device exhibits a maximum external quantum efficiency (EQE) of 4.2% with a slight roll‐off, indicating good charge balance due to the DA‐type molecular design. In the doped device with 4,4′‐bis(N‐carbazolyl)‐1,1′‐biphenyl (CBP) host, the BD3 exhibits higher EQE than 10% with Commission International de L'Eclairge (CIE) coordinates of (0.15, 0.06) and a narrow full‐width at half‐maximum of 45 nm, which is close to the CIE of the high definition television standard blue.