Post-mortem changes of connectin in chicken skeletal muscle

To investigate the influence of connectin (titin) on meat tenderization during conditioning, changes in the content and the electrophoretic pattern of the connectin in chicken myofibrils isolated by gel permeation chromatography were studied. Significant difference of the content and the electrophoretic pattern of the connectin isolated was not observed between the preparations from fresh and stored muscle. Therefore, the connectin is unlikely to be responsible for the meat tenderization caused by conditioning.     

Enhanced Superconductivity of the Pb-Based 1212 Cuprates in the (Pb0.75S0.25)(Sr2?xBax)(Y0.4Ca0.6)Cu2Oz System by High-Pressure O2 Annealing

We have already reported on substitution effects of Ba for Sr in the sulfur contained Pb-based 1212 compound with the composition of (Pb_0.75S_0.25)(Sr_2−x Ba _x ) (Y_0.4Ca_0.6)Cu₂O _z . It was found that each sample with x=0.3–0.6 showed a resistivity drop originating from superconductivity, and the highest onset temperature of the drop was about 32 K for a sample with x=0.5, but the volume fraction was very small. More recently, we have investigated effect of high-pressure O₂ annealing on superconductivity of the Ba-substituted samples with x=0.4–0.6. As a result, the sample with x=0.5 is found to show an onset of the resistivity drop at the highest temperature of about 41.7 K and it shows an onset of the diamagnetic signal at about 42.0 K. These transition temperatures are higher by ∼10 K than those of the previously reported sample with x=0.5. Moreover, the superconducting volume fraction is increasing more by the high-pressure O₂ annealing. These results indicate that the high-pressure O₂ annealing effectively promotes superconductivity of the present (Pb_0.75S_0.25)(Sr_2−x Ba _x )(Y_0.4Ca_0.6)Cu₂O _z system.     

Characterization of the NADH-linked acetylacetoin reductase/2,3-butanediol dehydrogenase gene from Bacillus cereus YUF-4

A 1.4-kbp DNA fragment, including the NADH-linked acetylacetoin reductase/2,3-butanediol dehydrogenase (AACRII/BDH) gene from the chromosomal DNA of Bacillus cereus YUF-4, was cloned in Escherichia coli DH5alpha after its insertion into pUC119, and the resulting plasmid was named pAACRII119. The AACRII/BDH gene had an open reading frame consisting of 1047 bp encoding 349 amino acids. The enzyme exhibited not only AACR activity, but also BDH activity. However, the gene was not located in a 2,3-butanediol (BD) operon, as is the case in the BDH gene of Klebsiella pneumoniae and that of K. terrigena. In addition, there was no BD-cycle-related enzyme gene in the region surrounding the AACRII/BDH gene. The AACR and BDH activities in E. coli DH5alpha/pAACRII119 were 200-fold higher than those in the original B. cereus YUF-4. The characteristics of the AACRII/BDH from E. coli DH 5alpha/pAACRII119 are similar to those of the AACRII/BDH from B. cereus YUF-4. The AACRII/BDH was considered to belong to the NAD(P)- and zinc-dependent long-chain alcohol dehydrogenase (group I ADH) family on the basis of the following distinctive characteristics: it possessed 14 strictly conserved residues of microbial group I ADH and consisted of about 350 amino acids. The enzymatic and genetic characteristics of AACRII/BDH were completely different from those of BDHs belonging to the short-chain dehydrogenase/reductase family. These findings indicated that the AACRII/BDH could be considered a new type of BDH.     

Polarization-Maintaining Optical Fibers Used for a Laser Diode Redundancy System in a Submarine Optical Repeater

Polarization-maintaining optical fibers are developed for installation in a submarine optical repeater. These fibers preserve the polarization of light emitted from laser diodes (LD's) to a single-mode fiber through a light-switching coupler. Crosstalk of less than -30 dB in a l-m length of these optical fibers, called PANDA optical fibers, is achieved with a bending radius greater than 10 mm, twists of less than 60 turns, and tension of less than 3 kg, and ambient temperatures of -20-80°C. The mechanical strength of these fibers is the same as that of single-mode fibers.     

In vivo observation of subendocardial microvessels of the beating porcine heart using a needle-probe videomicroscope with a CCD camera

We developed a portable needle-probe videomicroscope with a charge-coupled device (CCD) camera to visualize the subendocardial microcirculation. In 12 open-chest anesthetized pigs, the sheathed needle probe with a doughnut-shaped balloon and a microtube for flushing away the intervening blood was introduced into the left ventricle through an incision in the left atrial appendage via the mitral valve. Images of the subendocardial microcirculation of the beating heart magnified by 200 or 400 on a 15-in. monitor were obtained. The phasic diameter change in subendocardial arterioles during cardiac cycle was from 114 +/- 46 microns (mean +/- SD) in end diastole to 84 +/- 26 microns in end systole (p < 0.001, n = 13, ratio of change = 24%) and that in venules from 134 +/- 60 microns to 109 +/- 45 microns (p < 0.001, n = 15, ratio of change = 17%). In contrast, the diameter of subepicardial arterioles was almost unchanged (2% decrease, n = 5, p < 0.01), and the venular diameter increased by 19% (n = 8, p < 0.001) from end diastole to end systole. Partial kinking and/or pinching of vessels was observed in some segments of subendocardial arterioles and venules. The percentage of systolic decrease in the diameter from diastole in the larger (> 100 microns) subendocardial arterioles and venules was greater than smaller (50-100 microns) vessels (both p < 0.05). In conclusion, using a newly developed microscope system, we were able to observe the subendocardial vessels in diastole and systole.(ABSTRACT TRUNCATED AT 250 WORDS)     

Vibration control of flexible arm by multiple observer structure

This paper describes vibration control of a flexible arm by multiple‐observer structure using the accelerations of the arm. In general, a flexible arm has several oscillation modes. In the model of a flexible arm described as a two‐mass resonant system, the reaction torque feedback makes the flexible arm system stable. In the N‐mass resonant system, it is known that the reaction torque feedback makes all oscillation modes, only the reaction torque feedback is not enough to make the system stable. Resonance ratio control with the arm disturbance observer has been proposed. The arm disturbance observer is able to suppress the disturbance applied to the tip, but it is not sufficient for the disturbance applied to other points on the arm. The aim is to make the control system robust to arm inertia variation and disturbance. This paper proposes vibration control of flexible arm by acceleration feedback, and disturbance rejection by multiple‐observer structure using the acceleration of the arm. The validity of the proposed method is shown by simulations and experiments. © 2005 Wiley Periodicals, Inc. Electr Eng Jpn, 154(2): 68–75, 2006; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.20175