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101.
Micheletto R Denyer M Scholl M Nakajima K Offenhauser A Hara M Knoll W 《Applied optics》1999,38(31):6648-6652
We report the observation of live-cell dynamics by noncontact scanning near-field optical microscopy (SNOM) modified to work with living biological samples that are fully immersed in liquid. We did not use the SNOM setup in strictly near-field conditions (we used 1-mum constant-height mode); however, we could examine the dynamics of rhythmically beating cardiac myocytes in culture with extremely high vertical sensitivity below the nanometric range. We could halt scans at any point to record localized contraction profiles of the cell membrane. We show that the contractions of the organisms changed shape dramatically within adjacent areas. We believe that the spatial dependency of the contractions arises because of the measurement system's ability to resolve the behavior of individual submembrane actin bundles. Our results, combining imaging and real-time recording in localized areas, reveal a new, to our knowledge, noninvasive method for using SNOM setups for studying the dynamics of live biological samples. 相似文献
102.
H Shimada K Nakajima T Ochiai Y Koide SI Okazumi H Matsubara A Takeda Y Miyazawa M Arima K Isono 《Canadian Metallurgical Quarterly》1998,5(4):871-874
The significance of serum p53-Abs in patients with esophageal squamous cell carcinoma was determined. Examination of clinicopathological features and assessment of tumor marker sensitivities of carcinoembryonic antigen (CEA), squamous cell carcinoma antigen (SCC-Ag) and CYFRA21-1 were performed. Thirty-three (58%) of 57 patients were positive for serum p53-Abs, however, no relation with cancer progression existed. Fourteen of the 33 sero-positive patients revealed normal levels of all tumor markers tested. Thus, serum p53-Abs appears to be a useful marker for the detection of esophageal squamous cell carcinoma. 相似文献
103.
Remnants of chylomicron and very low density lipoprotein impair endothelium-dependent vasorelaxation
H Doi K Kugiyama M Ohgushi S Sugiyama T Matsumura Y Ohta T Nakano K Nakajima H Yasue 《Canadian Metallurgical Quarterly》1998,137(2):341-349
Given the multiple impairments in host defense that occur during HIV infection, patients with AIDS are at risk for a variety of pleural infections and neoplasms. Of infectious causes, bacterial parapneumonic effusions and empyemas and tuberculous pleurisy occur more frequently than effusions caused by P. carinii. In each case, therapy is directed at eradication of the causative organisms. In the setting of systemic Kaposi's sarcoma, pleural involvement is common, although diagnosis is difficult and therapeutic options are limited. Pleural effusions caused by non-Hodgkin's lymphoma often occur in the setting of pulmonary parenchymal disease and can be diagnosed cytologically. The recently described entity of primary effusion lymphoma occurs in the absence of solid-organ involvement. The development of a spontaneous pneumothorax in a HIV-infected individual should prompt a search for P. carinii infection. Although these pneumothoraces often recur and are difficult to manage, recent series suggest that surgical approaches to bronchopleural fistulas are reasonable in selected patients. 相似文献
104.
M Hisada T Fujita H Naoki Y Itagaki H Irie M Miyashita T Nakajima 《Canadian Metallurgical Quarterly》1998,36(8):1115-1125
Facile structure determination of acylpolyamines, glutamatergic nerve blocker obtained from the venom of the Joro spider (Nephila clavata) was carried out with the use of micro-column LC/MS and high energy collision induced dissociation (CID) mass spectrometry. 6-hydroxyindole-3-acetyl was proposed previously as a putative partial structure, for the acyl moiety of hydroxyindole-type polyamines (NPTX-1 to -6). The NMR data obtained for NPTX-6, NPTX-687 and hydroxyindole-3-acetic acid which was released by acid hydrolysis of Nephila clavata crude venom extracts proved that the lipophilic head is the 4-hydroxyindole-3-acetic acid. Various hydroxyindole-3-acetyl polyamines were found in N. Clavata venom and characterized by mass spectrometry. As a result, type-E, a new class of generalized acylpolyamine structure was proposed in addition to the previously reported polyamine backbones type-A to -D. 相似文献
105.
F Kawakami H Okamura Y Tamada T Nakajima Y Ibata 《Canadian Metallurgical Quarterly》1995,200(3):171-174
Photic stimulus and serotonin (5-hydroxytryptamine; 5-HT) are two factors known to regulate vasoactive intestinal peptide (VIP) synthesis in the suprachiasmatic nucleus (SCN). To explore the role of 5-HT in the photic stimulus-induced change in VIP synthesis, we investigated the changes in level of VIP mRNA under a 12 h light/12 h dark cycle following depletion of 5-HT by intraperitoneal administration of p-chlorophenylalanine (PCPA) methyl ester (200 mg/kg concentration) for 3 successive days. To estimate VIP mRNA expression, we performed in situ hybridization using imaging plates combined with microcomputer-based imaging analysis. In light-phase, total signals of VIP mRNA from the PCPA-treated rats showed a significant decrease compared with those from the saline-treated control rats. However, in dark-phase, there were no significant decreases between the PCPA-treated rats and the saline-control rats. The present results strongly suggest that 5-HT neuronal inputs to the SCN interfere with the effect of photic stimulus on VIP synthesis at the mRNA level. 相似文献
106.
107.
Summary Random-coiled conformation of poly(L-leucine), which has -branched side-chain, was theoretically analyzed by a conformational energy calculation based on intra-residue interactions. Calculated characteristic ratio 7.62 was obtained by using the transformation matrix statistically averaged over the entire side-chain conformational space of L-Leu residue. This value is smaller than those of poly(L-phenylalanine) (11.24) and poly(L-tyrosine) (12.33) which have -branched side-chain. 相似文献
108.
A new procedure is presented for determining in situ the solar calibration constant, i.e., the Sun-sky radiometer counts for a direct normal solar flux extrapolated to the top of the atmosphere. The method makes use of a modified version of the Langley plot based on the use of an inversion code of column-integrated aerosol size distribution, and it is ordinarily applied to calibrate Prede Sun-sky radiometers. To analyze how such an in situ method can work accurately, the technique has been applied to a five-month dataset obtained from measurements taken in Rome, Italy, by a Prede Sun-sky radiometer from 22 April to 5 November 2001. The precision of the in situ method has been estimated to within 1-2.5%, depending on the wavelength. 相似文献
109.
One modulation method called the frequency counting modulator (FCM), which can produce various constant envelope signals, is proposed. The FCM is materialized by only digital circuits and, therefore, is easily implemented in a large scale integrated circuit (LSI). From analytical study of the FCM, design formulas are derived for MSK-type modulation, and modulated signal performance features are clarified in terms of minimum signal distance, the effect of a Gaussian receiving filter, and spectral power concentration. 相似文献
110.
Nakajima N 《Applied optics》2003,42(14):2492-2497
A noniterative method of retrieving the two dimensional phase of a wave field from two intensity measurements is proposed. In the measurements, one records two far-field intensities of the wave field modulated and unmodulated with an exponential filter. The phase retrieval method is based on the solution of the simultaneous equations with unknown coefficients of the two-dimensional discrete Fourier transform for the phase. Then there is no need for the information about the wave field, which is used in iterative phase-retrieval methods. The usefulness of this method is shown in computer-simulated examples of the reconstruction of two-dimensional complex amplitude objects. 相似文献