Using multiple endmember spectral mixture analysis to retrieve subpixel fire properties from MODIS

The Moderate-Resolution Imaging Spectroradiometer (MODIS) sensors on NASA's Terra and Aqua satellites image most of the Earth multiple times each day, providing useful data on fires that cannot be practically acquired using other means. Unfortunately, current fire products from MODIS and other sensors leave large uncertainties in measurements of fire sizes and temperatures, which strongly influence how fires spread, the amount and chemistry of their gas and aerosol emissions, and their impacts on ecosystems. In this study, we use multiple endmember spectral mixture analysis (MESMA) to retrieve subpixel fire sizes and temperatures from MODIS, possibly overcoming some limitations of existing methods for characterizing fire intensities such as estimating the fire radiative power (FRP). MESMA is evaluated using data from the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) to assess the performance of FRP and MESMA retrievals of fire properties from a simultaneously acquired MODIS image, for a complex of fires in Ukraine from August 21, 2002. The MESMA retrievals of fire size described in this paper show a slightly stronger correlation than FRP does to fire pixel counts from the coincident ASTER image. Prior to this work, few studies, if any, had used MESMA for retrieving fire properties from a broad-band sensor like MODIS, or compared MESMA to higher-resolution fire data or other measures of fire properties like FRP. In the future, MESMA retrievals could be useful for fire spread modeling and forecasting, reducing hazards that fires pose to property and health, and enhancing scientific understanding of fires and their effects on ecosystems and atmospheric composition.     

MobSens: Making Smart Phones Smarter

In this article, we discuss experiences and lessons learned from deploying four mobile sensing applications on off-the-shelf mobile phones within a recreational framework called MobSens that contains elements of health, social, and environmental sensing at both individual and community levels. We describe the main components of our applications, which facilitate logging and external communications. We also outline the challenges faced when building and testing these applications and describe our strategies for overcoming them.     

Practice, systems and technology for seniors

This paper explores how research teams in Intel’s Digital Health Group are using ethnography to identify ‘designable moments’—spaces, times, objects, issues and practices which suggest opportunities for appropriate interventions. It argues that technology innovation should aim to incorporate the views, experiences and practices of users from the start of the design process to support independent living and develop culturally sensitive enhancements that contribute towards wellbeing and a life of quality for local older populations.     

Characterisation of flucloxacillin and 5-hydroxymethyl flucloxacillin haptenated HSA in vitro and in vivo

Flucloxacillin is a synthetic penicillin used in the treatment of Staphylococcal infections. Adverse reactions to the drug are believed to arise through covalent modification of proteins, with tissue damage occurring secondary to an immune reaction. Serum proteins have been shown by adduct-specific antibodies to be modified by flucloxacillin, but the nature and sites of modification have not been characterised. Here, in vitro studies on HSA have shown by MS that the modification of protein lysine residues occurs in a dose-, time- and site-dependent manner. Affinity, cation exchange and reversed phase chromatography prior to MS revealed in vivo modification of HSA with flucloxacillin in tolerant patients, with up to nine modified lysine residues being detected in each patient, and with modification of Lys190 and Lys212 being detected in 8/8 patients. It was also revealed for the first time that plasma proteins could be modified with the 5-hydroxymethyl metabolite of flucloxacillin, and that essentially the same Lys residues were targeted by both the parent drug and its metabolite. This study provides a detailed characterisation of sites of chemical modification of an endogenous target and reveals candidate peptides for T-cell and antibody assays of flucloxacillin hypersensitivity.     

Objective functional assessment of total hip arthroplasty following two common surgical approaches: the posterior and direct lateral approaches

Despite the high number of total hip arthroplasty (THA) procedures performed each year, there is no common consensus on the best surgical approach. Gait is known to improve following THA although it does not return to what is typically quantified as normal, and surgical approach is believed to be a contributing factor. The current study evaluates postoperative hip function and provides an objective assessment following two common surgical approaches: the McFarland-Osborne direct lateral and the southern posterior. Faced with the common problem of providing an objective comparison from the wealth of data collected using motion analysis techniques, the current study investigates the application of an objective classification tool to provide information on the effectiveness of each surgery and to differentiate between the characteristics of hip function following the two approaches. Seven inputs for the classifier were determined through statistical analysis of the biomechanical data. The posterior approach group exhibited greater characteristics of non-pathological gait and displayed a greater range of functional ability as compared with the lateral approach cohort. The classification tool has proved to be successful in characterizing non-pathological and THA function but was insufficient in distinguishing between the two surgical cohorts.     

Isolation and characterization of a monoclonal anti-quadruplex DNA antibody from autoimmune "viable motheaten" mice

A cell line that produces an autoantibody specific for DNA quadruplex structures has been isolated and cloned from a hybridoma library derived from 3-month-old nonimmunized autoimmune, immunodeficient "viable motheaten" mice. This antibody has been tested extensively in vitro and found to bind specifically to DNA quadruplex structures formed by two biologically relevant sequence motifs. Scatchard and nonlinear regression analyses using both one- and two-site models were used to derive association constants for the antibody-DNA binding reactions. In both cases, quadruplexes had higher association constants than triplex and duplex molecules. The anti-quadruplex antibody binds to the quadruplex formed by the promoter-region-derived oligonucleotide d(CGCG4GCG) (Ka = 3.3 x 10(6) M-1), and has enhanced affinity for telomere-derived quadruplexes formed by the oligonucleotides d(TG4) and d(T2G4T2G4T2G4T2G4) (Ka = 5.38 x 10(6) and 1.66 x 10(7) M-1, respectively). The antibody binds both types of quadruplexes but has preferential affinity for the parallel four-stranded structure. In vitro radioimmunofilter binding experiments demonstrated that purified anti-DNA quadruplex antibodies from anti-quadruplex antibody-producing tissue culture supernatants have at least 10-fold higher affinity for quadruplexes than for triplex and duplex DNA structures of similar base composition and length. The antibody binds intramolecular DNA triplexes formed by d(G4T3G4T3C4) and d(C4T3G4T3G4), and the duplex d(CGCGCGCGCG)2 with an affinities of 6. 76 x 10(5), 5.59 x 10(5), and 8.26 x 10(5) M-1, respectively. Competition experiments showed that melted quadruplexes are not effective competitors for antibody binding when compared to native structures, confirming that the quadruplex is bound structure-specifically. To our knowledge, this is the first immunological reagent known to specifically recognize quadruplex structures. Subsequent sequence analysis demonstrates homologies between the antibody complementarity determining regions and sequences from Myb family telomere binding proteins, which are hypothesized to control cell aging via telomeric DNA interactions. The presence of this antibody in the autoimmune repertoire suggests a possible linkage between autoimmunity, telomeric DNA binding proteins, and aging.     

Analysis of error-correction constraints in an optical disk

The compact disk read-only memory (CD-ROM) is a mature storage medium with complex error control. It comprises four levels of Reed Solomon codes allied to a sequence of sophisticated interleaving strategies and 8:14 modulation coding. New storage media are being developed and introduced that place still further demands on signal processing for error correction. It is therefore appropriate to explore thoroughly the limit of existing strategies to assess future requirements. We describe a simulation of all stages of the CD-ROM coding, modulation, and decoding. The results of decoding the burst error of a prescribed number of modulation bits are discussed in detail. Measures of residual uncorrected error within a sector are displayed by C1, C2, P, and Q error counts and by the status of the final cyclic redundancy check (CRC). Where each data sector is encoded separately, it is shown that error-correction performance against burst errors depends critically on the position of the burst within a sector. The C1 error measures the burst length, whereas C2 errors reflect the burst position. The performance of Reed Solomon product codes is shown by the P and Q statistics. It is shown that synchronization loss is critical near the limits of error correction. An example is given of miscorrection that is identified by the CRC check.     

New GOES imager algorithms for cloud and active fire detection and fire radiative power assessment across North, South and Central America

Vegetation fires are a key global terrestrial disturbance factor and a major source of atmospheric trace gases and aerosols. Therefore, many earth-system science and operational monitoring applications require access to repetitive, frequent and well-characterized information on fire emissions source strengths. Geostationary imagers offer important temporal advantages when studying rapidly changing phenomena such as vegetation fires. Here we present a new algorithm for detecting and characterising active fires burning within the imager footprints of the Geostationary Operational Environmental Satellites (GOES), including consideration of cloud-cover and calculation of fire radiative power (FRP), a metric shown to be strongly related to fuel consumption and smoke emission rates. The approach is based on a set of algorithms now delivering near real time (NRT) operational FRP products from the Meteosat Spinning Enhanced Visible and Infra-Red Imager (SEVIRI) imager (available from http://landsaf.meteo.pt/), and the GOES processing chain presented here is designed to deliver a compatible fire product to complete geostationary coverage of the Western hemisphere. Results from the two GOES imagers are intercompared, and are independently verified against the well regarded MODIS cloud mask and active fire products. We find that the detection of cloud and active fires from GOES matches that of MODIS very well for fire pixels having FRP > 30 MW, when the GOES omission error falls to less than 10%. The FRP of fire clusters detected near simultaneously by both GOES and MODIS have a bias of only 22 MW, and a similar bias is found when comparing near-simultaneous GOES East and GOES West FRP observations. However, many fire pixels having FRP < 30 MW remain undetected by GOES, probably unavoidably since it has a much coarser spatial resolution than MODIS. Adjustment using data from the less frequent but more accurate views obtained from high spatial resolution polar orbiting imagers could be used to bias correct regional FRP totals. Temporal integration of the GOES FRP record indicates that during the summer months, biomass burning combusts thousands of millions of tonnes of fuel daily across the Americas. Comparison of these results to those of the Global Fire Emissions Database (GFEDv2) indicate strong linear relationships (r² > 0.9), suggesting that the timely FRP data available from a GOES real-time data feed is likely to be a suitable fire emissions source strength term for inclusion in schemes aiming to forecast the concentrations of atmospheric constituents affected by biomass burning.     

Partitioning large networks without breaking communities

The identification of cohesive communities is a key process in social network analysis. However, the algorithms that are effective for finding communities do not scale well to very large problems, as their time complexity is worse than linear in the number of edges in the graph. This is an important issue for those interested in applying social network analysis techniques to very large networks, such as networks of mobile phone subscribers. In this respect, the contributions of this paper are twofold. First, we demonstrate these scaling issues using a prominent community-finding algorithm as a case study. Then, we show that a two-stage process, whereby the network is first decomposed into manageable subnetworks using a multilevel graph partitioning procedure, is effective in finding communities in networks with more than 10⁶ nodes.