Functional expression of the parathyroid cell calcium receptor in Xenopus oocytes

Various studies suggest the existence of a plasma membrane receptor on parathyroid cells that senses changes in the concentration of extracellular Ca2+. To test this hypothesis, Xenopus laevis oocytes were injected with poly(A)(+)-enriched mRNA from bovine parathyroid cells and examined for their ability to respond to increases in the concentration of extracellular Ca2+ or other polycations. Cytosolic Ca2+ concentrations were measured indirectly by recording Cl- currents through the endogenous, cytosolic Ca(2+)-activated Cl- channel. Increasing the concentration of extracellular Ca2+ (from 0.7 to 5 mM) or Mg2+ (from 0.8 to 10 mM) elicited oscillatory increases in the Cl- current. Responses to either divalent cation were not observed in oocytes injected with water or with mRNA prepared from HL-60 cells or rat liver. Responses elicited by extracellular Mg2+ persisted when extracellular Ca2+ was reduced to low micromolar levels. La3+, Gd3+, or neomycin B also evoked oscillatory increases in the Cl- current in oocytes under conditions of low extracellular Ca2+ levels. These extracellular polycations all cause the mobilization of intracellular Ca2+ in oocytes injected with parathyroid cell mRNA like they do in intact parathyroid cells. The injection of parathyroid cell mRNA thus confers on oocytes the ability to detect and respond to changes in the concentration of extracellular polycations. The data provide compelling evidence for the existence of a cell surface Ca2+ receptor protein(s) on parathyroid cells that regulates cellular function.     

Genetic evidence for mother-to-infant transmission of hepatitis G virus

Administration of a single high dose of methamphetamine (METH) causes a rapid and reversible decrease in the activity of the tryptophan hydroxylase (TPH), the rate-limiting enzyme in the synthesis of 5-hydroxytryptamine. This effect can be reversed completely by exposing the METH-impaired enzyme to a reducing environment, which suggests that the decrease in TPH activity is a reversible oxidative consequence of free radical formation. Consistent with this hypothesis, a single METH administration to male rats increased oxygen radical formation, as demonstrated by increased striatal dihydroxybenzoic acid formation after coadministration of salicylate with METH. Prevention of METH-induced hyperthermia attenuated both the increase in dihydroxybenzoic acid formation and the decrease in TPH activity observed 1 h after METH administration. These data suggest that both reactive oxygen species and hyperthermia contribute to the acute decrease in TPH activity which results from a single METH administration.     

Vitamin D status in growing dairy goats and sheep: Influence of ultraviolet B radiation on bone metabolism and calcium homeostasis

The aim of this study was to investigate how controlled UVB irradiation in combination with reduced nutritional vitamin D (vitD) supply affects vitD status and Ca metabolism of growing goats and sheep. The hypothesis was that, like dairy cows, goats and sheep are able to compensate for the missing nutritional supply of vitD through endogenous production in the skin, with the consequence of a high vitD status and a balanced Ca homeostasis. Sixteen lambs and 14 goat kids aged 3 and a half months were housed in an UVB free environment and fed hay and a vitD-free concentrate over a period of 13 wk. One group of each species was exposed to UVB lamps daily during individual feeding; the other groups served as controls. Serum, urine, and feces samples were taken at the start and at a monthly interval. Serum was analyzed for vitD metabolites, bone markers, growth hormone, insulin-like growth factor I, Ca, and P. Apparent digestibility and urinary excretion of Ca and P were determined. The left metatarsus was analyzed by peripheral quantitative computer tomography for bone mineral density before starting and at the end of the trial. In wk 13, all animals were slaughtered and samples of skin, rumen, duodenum, kidney, and bone (metatarsus) were collected. Content of sterols of vitD synthesis in the skin, Ca flux rates in rumen and duodenum, expression of vitD receptor in duodenum and kidney, renal and intestinal gene expression of Ca transport proteins, and renal enzymes related to vitD metabolism were determined. The UVB exposure led to lower 7-dehydrocholesterol content in the skin and a better vitD status (higher serum 25-hydroxyvitamin D), but no signs of vitD deficiency were seen in the control groups and no effect of irradiation was detected in the analyzed parameters of Ca homeostasis. Differences between the 2 species were detected: lambs had a higher increase of bone mineral density, lower values of bone markers, growth hormone, and insulin-like growth factor I in serum and higher tachysterol and lower lumisterol content in skin compared with goat kids. The results indicated that growing lambs and goat kids are able to compensate for a vitD-reduced diet by cutaneous vitD synthesis when exposed to UVB irradiation and therefore to keep a high vitD status. In contrast, when a reduced vitD diet is combined with missing UVB exposure, the vitD status drops, but the experimental time was probably too short to induce a vitD deficiency or an effect on Ca homeostasis.     

Micropolar beam models for lattice grids with rigid joints

A simple, rational approach is presented for developing micropolar beam models for large repetitive beam-like planar lattices with rigid joints. The micropolar beam models have independent microrotation, and displacement fields and are characterized by their strain and kinetic energies, from which the equations of motion and boundary conditions can be derived. The procedure for developing the expression for the strain energy of the micropolar beam involves introducing basic assumptions regarding the variation of the displacement and microrotation components in the plane of the cross-section and obtaining effective elastic coefficients of the continuum in terms of the material properties and geometry of the original lattice structure. The high accuracy of the solutions obtained by the micropolar beam models is demonstrated by means of numerical examples.     

Spontaneous perseverative turning in rats with radiation-induced hippocampal damage.

This study found a new behavioral correlate of lesions specific to the dentate granule cell layer of the hippocampus: spontaneous perseverative turning. Irradiation of a portion of the neonatal rat cerebral hemispheres produced hypoplasia of the granule cell layer of the hippocampal dentate gyrus while sparing the rest of the brain. Radiation-induced damage to the hippocampal formation caused rats placed in bowls to spontaneously turn in long, slow bouts without reversals. Irradiated subjects also exhibited other behaviors characteristic of hippocampal damage (e.g., perseveration in spontaneous exploration of the arms of a T-maze, retarded acquisition of a passive avoidance task, and increased horizontal locomotion). These data extend previously reported behavioral correlates of fascia dentata lesions and suggest the usefulness of a bout analysis of spontaneous bowl turning as a measure of nondiscrete-trial spontaneous alternation and a sensitive additional indicator of radiation-induced hippocampal damage. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Bounds on Flexural Properties and Buckling Response for Symmetrically Laminated Composite Plates

Nondimensional parameters and equations governing the buckling behavior of rectangular symmetrically laminated plates are presented that can be used to represent the buckling resistance, for plates made of all known structural materials, in a very general, insightful, and encompassing manner. In addition, these parameters can be used to assess the degree of plate orthotropy, to assess the importance of anisotropy that couples bending and twisting deformations, and to characterize quasi-isotropic laminates quantitatively. Bounds for these nondimensional parameters are also presented that are based on thermodynamics and practical laminate construction considerations. These bounds provide insight into potential gains in buckling resistance through laminate tailoring and composite-material development. As an illustration of this point, upper bounds on the buckling resistance of long rectangular orthotropic plates with simply supported or clamped edges and subjected to uniform axial compression, uniform shear, or pure in-plane bending loads are presented. The results indicate that the maximum gain in buckling resistance for tailored orthotropic laminates, with respect to the corresponding isotropic plate, is in the range of 26–36% for plates with simply supported edges, irrespective of the loading conditions. For the plates with clamped edges, the corresponding gains in buckling resistance are in the range of 9–12% for plates subjected to compression or pure in-plane bending loads and potentially up to 30% for plates subjected to shear loads.     

Groups at work: lessons from research into large-scale coordination

Study of large-scale coordination seeks us to understand work between and across groups beyond earlier conceptions of social human factors. Revealing the complexity of work domains and the subtle yet effective ways that workers negotiate its challenges opens the way to develop support tools that are grounded in a scientific understanding. This first special issue in Large-Scale Coordination shows the breadth of application research from air traffic control to the military and multi-agency emergency response. A second special issue later this year will show depth of research at large scale in healthcare.     

Evaluation of Ah Genotype on PAH: DNA Binding and Metabolite Excretion in Mice Fed Coal Tar

Female C57BL/6J and DBA/2J mice were fed a diet containing 0.3% coal tar for 14 days. Urine was collected during the last 24 hr of diet administration for metabolite analysis. Lung and liver tissues were evaluated for chemical:DNA adduct formation using 32 P-postlabeling. 1-Hydroxypyrene levels were 3.5 times greater in urine of DBA/2J than C57BL/6J mice, while a difference of only 1.3-fold was observed for urinary 3-hydroxybenzo[ a ]pyrene. Lung chemical:DNA adduct levels were greater in C57BL/6J than DBA/2J mice, with averages of 0.33 - 0.47 and 0.16 - 0.12 pmole adducts/mg DNA, respectively. Chemical:DNA adduct levels for liver tissues, however, were 2.5 times greater for DBA/2J than C57BL/6J mice. These results are contrary to those obtained in previous studies where a single hydrocarbon was administered. The abundance of PAHs present in coal tar may have evoked metabolic responses not governed by the Ah receptor, thereby accounting for the contradictory results.     

BUCKLING BEHAVIOR OF LONG ANISOTROPIC PLATES SUBJECTED TO RESTRAINED THERMAL EXPANSION AND MECHANICAL LOADS

An approach for synthesizing buckling results and behavior for thin balanced and unbalanced symmetric laminates that are subjected to nondestabilizing mechanical preloads, uniform heating or cooling, and elastically or totally restrained axial thermal expansion or contraction is presented. This approach uses a nondimensional analysis for infinitely long, flexurally anisotropic plates that are subjected to combined loads and is based on useful nondimensional parameters. In addition, a stiffness-weighted laminate coefficient of thermal expansion is derived and used to determine critical temperatures in terms of physically intuitive mechanical buckling coefficients, and the effects of membrane orthotropy and membrane anisotropy are included. Many results are presented for some common laminates that are intended to facilitate a structural designer's transition to the use of the generic buckling design curves that are presented in the article. Several generic buckling design curves are presented that provide physical insight into the buckling response in addition to providing useful design data. Examples are presented that demonstrate the use of the generic design curves. The analysis approach and generic results identify the effects and characteristics of laminate thermal expansion, membrane orthotropy and anisotropy, and flexural orthotropy and anisotropy in a very general and unifying manner.