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101.
Ivana Rubi Richard Burchmore Stefan Weidt Clement Regnault Josipa Kule Renata Bari Rafaj Tomislav Maek Anita Horvati Martina Crnogaj Peter David Eckersall Predrag Novak Vladimir Mrljak 《International journal of molecular sciences》2022,23(3)
Canine babesiosis is an important tick-borne disease worldwide, caused by parasites of the Babesia genus. Although the disease process primarily affects erythrocytes, it may also have multisystemic consequences. The goal of this study was to explore and characterize the serum metabolome, by identifying potential metabolites and metabolic pathways in dogs naturally infected with Babesia canis using liquid and gas chromatography coupled to mass spectrometry. The study included 12 dogs naturally infected with B. canis and 12 healthy dogs. By combining three different analytical platforms using untargeted and targeted approaches, 295 metabolites were detected. The untargeted ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC-MS/MS) metabolomics approach identified 64 metabolites, the targeted UHPLC-MS/MS metabolomics approach identified 205 metabolites, and the GC-MS metabolomics approach identified 26 metabolites. Biological functions of differentially abundant metabolites indicate the involvement of various pathways in canine babesiosis including the following: glutathione metabolism; alanine, aspartate, and glutamate metabolism; glyoxylate and dicarboxylate metabolism; cysteine and methionine metabolism; and phenylalanine, tyrosine, and tryptophan biosynthesis. This study confirmed that host–pathogen interactions could be studied by metabolomics to assess chemical changes in the host, such that the differences in serum metabolome between dogs with B. canis infection and healthy dogs can be detected with liquid chromatography-mass spectrometry (LC-MS) and gas chromatography-mass spectrometry (GC-MS) methods. Our study provides novel insight into pathophysiological mechanisms of B. canis infection. 相似文献
102.
Pawel Grochecki Irena Smaga Paulina Surowka Marta Marszalek-Grabska Predrag Kalaba Vladimir Dragacevic Patrycja Kotlinska Malgorzata Filip Gert Lubec Jolanta H. Kotlinska 《International journal of molecular sciences》2022,23(18)
Maternal separation (MS) is a key contributor to neurodevelopmental disorders, including learning disabilities. To test the hypothesis that dopamine signaling is a major factor in this, an atypical new dopamine transporter (DAT) inhibitor, CE-123, was assessed for its potential to counteract the MS-induced spatial learning and memory deficit in male and female rats. Hence, neonatal rats (postnatal day (PND)1 to 21) were exposed to MS (180 min/day). Next, the acquisition of spatial learning and memory (Barnes maze task) and the expression of dopamine D1 receptor, dopamine transporter (DAT), and the neuronal GTPase, RIT2, which binds DAT in the vehicle-treated rats were evaluated in the prefrontal cortex and hippocampus in the adolescent animals. The results show that MS impairs the acquisition of spatial learning and memory in rats, with a more severe effect in females. Moreover, the MS induced upregulation of DAT and dopamine D1 receptors expression in the prefrontal cortex and hippocampus in adolescent rats. Regarding RIT2, the expression was decreased in the hippocampus for both the males and females, however, in the prefrontal cortex, reduction was found only in the females, suggesting that there are region-specific differences in DAT endocytic trafficking. CE-123 ameliorated the behavioral deficits associated with MS. Furthermore, it decreased the MS-induced upregulation of D1 receptor expression level in the hippocampus. These effects were more noted in females. Overall, CE-123, an atypical DAT inhibitor, is able to restore cognitive impairment and dopamine signaling in adolescent rats exposed to MS—with more evident effect in females than males. 相似文献
103.
Ana Ilic Dusan Todorovic Slavica Mutavdzin Novica Boricic Biljana Bozic Nedeljkovic Sanja Stankovic Tatjana Simic Predrag Stevanovic Vera Celic Dragan Djuric 《International journal of molecular sciences》2021,22(6)
The possible cardioprotective effects of translocator protein (TSPO) modulation with its ligand 4′-Chlorodiazepam (4′-ClDzp) in isoprenaline (ISO)-induced rat myocardial infarction (MI) were evaluated, alone or in the presence of L-NAME. Wistar albino male rats (b.w. 200–250 g, age 6–8 weeks) were divided into 4 groups (10 per group, total number N = 40), and certain substances were applied: 1. ISO 85 mg/kg b.w. (twice), 2. ISO 85 mg/kg b.w. (twice) + L-NAME 50 mg/kg b.w., 3. ISO 85 mg/kg b.w. (twice) + 4′-ClDzp 0.5 mg/kg b.w., 4. ISO 85 mg/kg b.w. (twice) + 4′-ClDzp 0.5 mg/kg b.w. + L-NAME 50 mg/kg b.w. Blood and cardiac tissue were sampled for myocardial injury and other biochemical markers, cardiac oxidative stress, and for histopathological evaluation. The reduction of serum levels of high-sensitive cardiac troponin T hs cTnT and tumor necrosis factor alpha (TNF-α), then significantly decreased levels of serum homocysteine Hcy, urea, and creatinine, and decreased levels of myocardial injury enzymes activities superoxide dismutase (SOD) and glutathione peroxidase (GPx) as well as lower grades of cardiac ischemic changes were demonstrated in ISO-induced MI treated with 4′-ClDzp. It has been detected that co-treatment with 4′-ClDzp + L-NAME changed the number of registered parameters in comparison to 4′-ClDzp group, indicating that NO (nitric oxide) should be important in the effects of 4′-ClDzp. 相似文献
104.
Abu Talib Ruhiyati Idayu Nikolic Predrag K. Sunar Mohd Shahrizal Prada Rui 《Mobile Networks and Applications》2020,25(3):913-924
Mobile Networks and Applications - In this paper, we were discussing designed processes in prototyping In-Visible Island: Inclusive Storytelling Platform for Visually Impaired Children to... 相似文献
105.
Miščević Predrag Vlastelica Goran 《Bulletin of Engineering Geology and the Environment》2019,78(3):1843-1853
Bulletin of Engineering Geology and the Environment - Embankments made from crushed soft rock grains can be susceptible to additional settlement caused by a deterioration of the grains inside the... 相似文献
106.
107.
Predrag N. Ivanis Milena M. Stojnic Dusan B. Drajic 《AEUE-International Journal of Electronics and Communications》2013,67(2):113-122
Using a finite state Markov channel model, we develop an analytical method for evaluation of the packet error structure in multiple-input multiple-output (MIMO) systems based on singular value decomposition (SVD). We consider dual-branch MIMO systems, with either two transmit and arbitrary number of receive antennas, or arbitrary number of transmit and two receive antennas. The corresponding Markov model parameters are obtained using a novel closed-form expressions for probability density function and level crossing rate of the signal-to-noise ratio at the output of eigenchannels in a MIMO system, derived for a case of Rayleigh propagation, imperfect channel state information and any fixed power allocation. The exact bit error rate for the transmission of quadrature amplitude modulated (QAM) symbols through the eigenchannels is derived in polynomial closed form. Furthermore, by using the developed Markov model, the packet error statistics in the corresponding eigenchannels are determined, and the closed-form analytical expression for the system throughput is derived when ‘go-back-N’ automatic repeat request procedure is applied in time-varying eigenchannels. The analytical results are validated by using Monte Carlo simulations. 相似文献
108.
Novica Sovtić Kojić S. Predrag Oskar J. Bera Jelena M. Pavličević Olga M. Govedarica Mirjana C. Jovičić Dragan D. Govedarica 《Polymer Engineering and Science》2020,60(6):1097-1117
Vegetable oils were investigated as plasticizers to improve the sustainability of rubber compounding. The potential use of different vegetable oils as an alternative to petroleum-based rubber process oils was reviewed. This article presents a literature review on the current understanding of the influence of vegetable oil properties on natural rubber (NR) compounding. Hansen solubility parameters of the vegetable oils were determined to assist with the selection of vegetable oil for NR. We believe that the use of Hansen parameters could make the use of vegetable oils in rubber more convenient and cost-effective. 相似文献
109.
Dielectric characteristics of vacuum circuit breakers with CuCr and CuBi contacts before and after short-circuit breaking operations 总被引:1,自引:0,他引:1
Dragan Mosti? Predrag Osmokrovi? Koviljka Stankovi? Radovan Radosavljevi? 《Vacuum》2011,86(2):156-164
This paper presents the results of the degree of irreversible changes of dielectric properties of vacuum circuit breakers with CuCr and CuBi contacts before and after short-circuit breaking operations. Tests of dielectric properties were performed on four different types of switching vacuum chamber with RMF electrodes in the form of a spiral disk with slots. The paper describes the configuration of measuring system for determining the dielectric properties of circuit breakers with DC, AC and pulse voltage. It also describes the measuring procedure. The results were analyzed and the experimentally obtained random variable breakdown voltage is found to belong to Weibull distribution in all cases. Based on these results it was found that for the vacuum circuit breakers with CuCr contacts and CuBi breakdown occurs by the emission mechanism after stopping of diffuse arc with the initial breakdown at the anode. It was also found that significant reduction of the breakdown voltage occurs only after a short-circuit current breaking for a constricted arc. Only in this case, the significant difference between circuit breakers with CuBi and CuCr contacts is observed. This difference is explained by the fact that breakdown voltage for a constricted arc strongly depends on the shape of the applied voltage by the action mechanism of micro particles generated during the breaking of the arc current for the vacuum circuit breakers with CuBi contacts and it is caused by material of contacts. The results confirm the lower degree of irreversible changes of dielectric properties of vacuum circuit breakers with CuCr contacts in operation. 相似文献
110.
We present here a study on overlooked aspects of alternating current (AC) electrokinetics-AC electrophoretic (ACEP) phenomena. The dynamics of a particle with both polarizability and net charges in a non-uniform AC electric trapping field is investigated. It is found that either electrophoretic (EP) or dielectrophoretic (DEP) effects can dominate the trapping dynamics, depending on experimental conditions. A dimensionless parameter γ is developed to predict the relative strength of EP and DEP effects in a quadrupole AC field. An ACEP trap is feasible for charged particles in 'salt-free' or low salt concentration solutions. In contrast to DEP traps, an ACEP trap favors the downscaling of the particle size. 相似文献