Rapid analytical tryptic mapping of a recombinant chimeric monoclonal antibody and method validation challenges

A rapid and reproducible analytical tryptic mapping method was developed as an identity test for a recombinant chimeric monoclonal antibody for lot release testing. The unfolding, reduction, carboxymethylation, trypsin digestion, and reversed-phase (RP) HPLC steps were optimized to provide a reproducible method. The optimized method requires 30 min for unfolding the protein, 30 min for carboxymethylation, 4 h for digestion with TPCK-trypsin and 140 min for RPHPLC analysis. The total time required is less than 8 h compared to conventional procedures, which must be performed over several days. The optimized method was validated for its precision, recovery, specificity, and robustness. The precision of the method was determined by repeatability and intermediate precision experiments. Relative standard deviation (RSD) values were < or = 10% for the relative peak areas of marker peaks. The mean recovery of these marker peaks was 88.4%. The specificity was demonstrated by the unique tryptic mapping patterns obtained compared with several other monoclonal antibodies. Robustness was demonstrated by the relative insensitivity of the tryptic map to small deliberate changes in key method parameters. Excessive relative peak area variability observed for one peak (RSD 52%) was traced to adsorption to glass autosampler vials. This variability was substantially reduced (RSD 11%) by substituting polypropylene autosampler vials. The data demonstrate that this method may be applicable to a wide range of pharmaceutically relevant monoclonal antibodies.     

Role of magnesium in cast aluminium alloy matrix composites

Wetting between the dispersoid and the matrix alloy is the foremost requirement during the preparation of metal matrix composites (MMC) especially with the casting/liquid metal processing technique. The basic principles involved in improving wetting fall under three categories: (i) increasing the surface energies of the solids, (ii) decreasing the surface tension of the liquid matrix alloy, and (iii) decreasing the solid/liquid interfacial energy at the dispersoid matrix interface. The presence of magnesium, a powerful surfactant as well as a reactive element, in the aluminium alloy matrix seems to fulfil all the above three requirements. The role played by magnesium during the synthesis of aluminium alloy matrix composites with dispersoids such as zircon (ZrSiO₄), zirconia (ZrO₂), titania (TiO₂), silica (SiO₂), graphite, aluminium oxide (Al₂O₃) and silicon carbide (SiC), has been analysed. The important role played by the magnesium during the composite synthesis is the scavenging of the oxygen from the dispersoid surface, thus thinning the gas layer and improving wetting and reaction-aided wetting with the surface of the dispersoid. The combinations of magnesium and aluminium seem to have some synergistic effect on wetting.     

Similarity considerations in the physical modelling of steel making tundish systems

To assess and quantify the relative importance of Reynolds and Froude numbers in reduced scale model studies (these cannot be simultaneously respected when the scale factor is less than unity), aqueous model investigations were carried out on three different laboratory scale tundish models. The experimental tundish systems included two strand, five strand skewed delta shaped and six strand rectangular shaped vessels. Experimental observations show that the depth of liquid in the model would only correspond to that in the full scale system, provided the model flow rate is scaled in accordance with the relationship: Q_m = λ^5/2 Q_f.S, in which, A is the geometrical scaling factor. Furthermore, on the basis of residence time distribution measurements in two different configurations of the five strand tundish, it was demonstrated explicitly that flow phenomena in tundish systems are largely dominated by inertial forces and are therefore, essentially Froude dominated.     

Evaluation of materials for artificial heart valves

The heart of a normal human being beats about 38 million cycles every year. An artificial heart valve, to perform at this rate in the adverse conditions inside the heart for 20 years or more, should be highly wear-resistant with excellent fatigue strength. Thus, the study of mechanical and physical properties of the materials intended for use in artificial valves becomes an inseparable part of the valve development process itself. The physical and mechanical requirements of the materials used in the Chitra heart valve have been evaluated by studying their water absorption, adhesive wear and abrasive properties. The mechanical durability of the device has been assessed by accelerated life cycle testing. The test systems developed for the above are described here. The results show UHMW-PE to be a highly wear-resistant material suitable for the occluder. The accelerated wear tests show that the valve with Haynes-25 alloy cage and UHMW-PE disc has durability in excess of 50 years.     

Ratchet effect study in Si/SiGe heterostructures in the presence of asymmetrical antidots for different polarizations of microwaves

In this work, we studied the photovoltage response of an antidot lattice to microwave radiation for different antidot parameters. The study was carried out in a Si/SiGe heterostructure by illuminating the antidot lattice with linearly polarized microwaves and recording the polarity of induced photovoltage for different angles of incidence. Our study revealed that with increased antidot density and etching depth, the polarity of induced photovoltage changed when the angle of incidence was rotated 90 degrees. In samples with large antidot density and/or a deeply etched antidot lattice, scattering was dominated by electron interaction with the asymmetrical potential created by semicircular antidots. The strong electron–electron interaction prevailed in other cases. Our study provides insight into the mechanism of interaction between microwaves and electrons in an antidot lattice, which is the key for developing an innovative ratchet-based device. Moreover, we present an original and fundamental example of antidot lattice etching through the use of a two-dimensional electron gas. This system deals with a hole lattice instead of an electron depletion in the antidot lattice region.     

High-purity nano silica powder from rice husk using a simple chemical method

A highly pure, small particle-sized and high surface area nano silica powder was prepared from rice husk using alkali extraction, followed by an acid precipitation method. The composition, phase, morphology, size and surface area of the as-synthesised nano silica powder was investigated by energy-dispersive spectroscopy, Fourier transform infrared spectroscopy, X-ray diffraction, transmission electron microscopy, particle size analyser and BET surface area analyser. High-purity nano silica powder was obtained by sodium hydroxide (NaOH) purification treatment (0.5, 1, 1.5, 2 and 2.5?N). The high purity of silica (～99.9%) was obtained at 2.5?N NaOH treatment. The pure nano silica powder that is obtained shows an average particle size of ～25?nm with a high-specific surface area (SSA) of 274?m²?g^?1, with an average pore diameter of 1.46?nm.     

Cross-linked poly(vinyl alcohol) and poly(styrene sulfonic acid-co-maleic anhydride)-based semi-interpenetrating network as proton-conducting membranes for direct methanol fuel cells

A series of semi-interpenetrating network (SIPN) membranes was synthesized by using poly(vinyl alcohol) (PVA) with sulfosuccinic acid (SSA) as a cross-linking agent and poly(styrene sulfonic acid-co-maleic acid) (PSSA-MA) as a proton source for direct methanol fuel cell (DMFC) application. A bridge of SSA between PVA molecules not only reinforced the network but also provided extra proton-conducting paths. PSSA-MA chains trapped in the network were the major proton conduction path of the membrane. The SIPN membranes with 80% PSSA-MA (SIPN-80) exhibited a higher proton conductivity value of 2.59 × 10⁻² S cm⁻¹ and very low methanol permeability (4.1 × 10⁻⁷ cm² s⁻¹). More specifically, the SIPN membranes exhibited very high selectivity (proton conductivity/methanol permeability). Membrane characteristics such as water uptake, proton conductivity and methanol permeability were evaluated to determine the effect of PVA molecular weights. The SIPN membranes with higher PVA molecular weight were also evaluated using methanol and oxygen gas in a single cell fuel cell at various temperatures. Power density value of over 100 mW cm⁻² was obtained for SIPN membrane-based membrane electrode assembly at 80 °C and using commercial binary alloy anode catalysts and 2 M methanol.     

In vitro degradation behaviour of a friction stir processed magnesium alloy

In this study, the in vitro degradation behaviour of a friction stir processed AZ31 magnesium alloy was investigated. Electrochemical experiments in simulated body fluid suggest that friction stir processing marginally enhances the degradation resistance of the alloy, which could be attributed to the dissolution of secondary phase particles. Homogenisation of the microstructure reduces galvanic corrosion. It is envisaged that the beneficial effect would be more pronounced for magnesium alloys which contain high volume fraction of galvanic corrosion inducing secondary phase particles.     

Use of flip ambiguity probabilities in robust sensor network localization

Collinear or near collinear placement of some sensors in a wireless sensor network causes the location estimates of nearby sensors to be sensitive to erroneous distance measurements which leads to large location estimation errors. These errors and the possible propagation of these errors to the entire network or a large portion of it, thereby causing larger estimation errors for some sensors’ locations, is a major problem in localization. This phenomenon is well described in rigid graph theory, using the notion of “flip ambiguity”. This paper considers arbitrary sensor neighborhoods of two dimensional sensor networks and formulates an analytical expression for the probability of occurrence of the flip ambiguity. Based on the derived probability expression, a methodology is proposed to make the localization algorithms robust by calculating such flip ambiguity probabilities and eliminating potentially poor location estimates as well as assigning confidence factors to the estimated locations to prevent them from ruining the subsequent localization steps. The efficiency of the proposed methodology is demonstrated via a set of simulations.     

Photo-switching and nonlinear optical behaviors of center linked bent-core azobenzene liquid crystalline polymers

A new class of two series (I and II) of center linked bent-core azobenzene liquid crystalline polymers were prepared. They were prepared from two different spacer lengths (2 and 10) between polymer backbone and bent-core mesogen. The bent-core mesogen possesses photoactive linking group with variable terminal chains (7, 8, and 9). The synthesized precursors, monomers, and polymers were characterized by FT-IR, ¹H NMR, and ¹³C NMR spectroscopy. Thermal stability of polymers was examined by thermogravimetric analysis. The mesophase transition of monomers and polymers were observed through polarized optical microscopy, and confirmed by differential scanning calorimetry. Among the two series of polymers, the second series of polymers possesses liquid crystalline properties. The photo-switching properties of bent-core azo polymers were investigated using UV–Vis spectroscopy, trans to cis isomerization occurs around 35 s in chloroform and 65 s in thin film, where as reverse processes take place almost 32 h in chloroform. The photo-switching processes of polymer IIa precedes faster and also longer time thermally stable when compared with recently reported similar polymers. Negative optical nonlinear refractive index and optical limiting properties of the polymers were measured using Nd-YAG laser.