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21.
G Egusa F Murakami C Ito Y Matsumoto S Kado M Okamura H Mori K Yamane H Hara M Yamakido 《Canadian Metallurgical Quarterly》1993,100(2):249-255
It has recently been reported that there exists a new 'RING-finger' protein family among the zinc-finger (Zf) proteins. Previously, we had isolated the mouse Mel-18 cDNA (mMel-18) encoding the nuclear RING-finger protein that exhibits an ability to bind to a nonspecific DNA column. Here, we have isolated and characterized the human Mel-18 cDNA (hMel-18) using the mMel-18 cDNA as a probe. The deduced hMel-18 protein contains 344 amino acids (38 kDa) with a RING-finger motif, a helix-loop-helix (HLH)-like structure and a Pro/Ser-rich region. The hMel-18 gene is conserved among vertebrates. Its mRNA is highly expressed in placenta, lung and kidney, but the level is low in liver, pancreas and skeletal muscle. Using in situ hybridization, we mapped hMel-18 to band q22 of chromosome 12. It is possible that the Mel-18/bmi-1 gene family represents a mammalian homologue of the Drosophila polycomb gene group. 相似文献
22.
The microporous polypropylene sheets were prepared by biaxially stretching polypropylene sheets containing CaCO3 filler (particle size, 0.08–3.0 μm), when the CaCO3 filler content was 59% by weight and the stretching ratio was 2.8 × 1.8. The microstructure of the sheets were investigated in relation to the CaCO3 particle size by a N2 gas permeation method. (1) Effective porosity increases with decreasing mean particle size of filler. (2) The tortuosity factor of the pore is in the range of 25–40 and becomes relatively smaller with decreasing mean particle size of filler. (3) The equivalent pore size becomes relatively smaller with decreasing mean particle size of filler. 相似文献
23.
Phenol novolac/poly (4-hydroxyphenylmaleimide) (PHPMI) blends were used as an epoxy resin hardener. The curing behavior of the above system and the thermal and mechanical properties of the cured epoxy resin were studied. It was not necessary to use a curing accelerator for this system, because PHPMI caused acceleration of the curing reaction. The curing mechanism of this system was investigated by using model compounds. Test pieces from the neat resins and the glass fiber reinforced resins were evaluated in terms of thermal and mechanical properties, respectively. It was found that heat resistance and mechanical properties were improved by increasing the amount of PHPMI in the hardener. 相似文献
24.
Satoshi Takeuchi Ryo Sugihara 《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》1998,410(3):104-508
The longitudinal electric field of single and double Gaussian laser beams are used to accelerate electrons. The longitudinal field of the single beam is concentrated on the axis and is favourable for acceleration. A set of two beams is considered. Beams run parallel, collinearly, overlap partially and have a phase difference iπ in between. As a result, the transverse components of fields cancel each other while the longitudinal components are double-fold. In both schemes, the electrons are accelerated in lengths of the Rayleigh range, which is common to the plasma-based accelerators. 相似文献
25.
S Fukumoto M Suzawa Y Takeuchi Y Kodama K Nakayama E Ogata T Matsumoto T Fujita 《Canadian Metallurgical Quarterly》1996,81(7):2554-2558
26.
K. Matsumoto T. Ikegami S. Ito M. Kirigaya Y. Okuda 《Journal of Low Temperature Physics》1996,102(1-2):227-235
A precise velocity measurement of zero and first sound in normal3He was performed over the pressure range of 0.5 bar and 10 bar at a frequency of 389.1 MHz using a phase detection technique. From these measurements, the Fermi liquid parameter F
2
s
was determined as a function of pressure. The new value of F
2
s
was positive and less than 0.25 at all pressures below 10 bar. It allows propagation of transverse zero sound mode for all pressures within the current theoretical picture. 相似文献
27.
28.
JS Lee S Scala Y Matsumoto B Dickstein R Robey Z Zhan G Altenberg SE Bates 《Canadian Metallurgical Quarterly》1997,65(4):513-526
MCF-7 human breast cancer cells selected in Adriamycin in the presence of verapamil developed a multidrug resistant phenotype, which was characterized by as much as 100,000-fold resistance to mitoxantrone, 667-fold resistance to daunorubicin, and 600-fold resistance to doxorubicin. Immunoblot and PCR analyses demonstrated no increase in MDR-1 or MRP expression in resistant cells, relative to parental cells. This phenotype is similar to one previously described in mitoxantrone-selected cells. The cells, designated MCF-7 AdVp, displayed a slower growth rate without alteration in topoisomerase II alpha level or activity. Increased efflux and reduced accumulation of daunomycin and rhodamine were observed when compared to parental cells. Depletion of ATP resulted in complete abrogation of efflux of both daunomycin and rhodamine. No apparent alterations in subcellular daunorubicin distribution were observed by confocal microscopy. No differences were noted in intracellular pH. Molecular cloning studies using DNA differential display identified increased expression of the alpha subunit of the amiloride-sensitive sodium channel in resistant cells. Quantitative PCR studies demonstrated an eightfold overexpression of the alpha subunit of the Na+ channel in the resistant subline. This channel may be linked to the mechanism of drug resistance in the AdVp cells. The results presented here support the hypothesis that a novel energy-dependent protein is responsible for the efflux in the AdVp cells. Further identification awaits molecular cloning studies. 相似文献
29.
30.
The codeword error rate (WER) performance of noncoherent frequency-shift keying with soft decision decoding of block codes using Chase's second algorithm (1972) is investigated in a Rayleigh fading channel. The received signal envelope is sampled and used as channel measurement information. The theoretical upper and lower bounds of the WER are derived, assuming independent Rayleigh envelope samples in a received block. When the Golay (23, 12, 7) code is used, soft decision decoding with 6-bit error correction capability (3-bit error and 3-bit erasure) requires an average signal-to-noise power ratio about 5 dB lower than that for minimum distance decoding with 3-bit error correction to obtain a WER=10-3. The effects of bit interleaving on the WER performance when fading envelope variation is slow compared to the bit rate are investigated through computer simulations 相似文献