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111.
Effects of extrusion ratio (ER) during extruding on mechanical properties of poly(lactic acid) (PLA) screws were investigated in terms of shear and twist strength. Shear strength increased by drawing, while twist strength decreased. This might be due to orientation of molecular chains during drawing. In order to evaluate orientation of molecular chains, orientation function in extruded billets was measured as a function of ER. Since orientation function increased with ER, drawing is effective method to improve shear strength of screw, where fibrous structure which was formed during extruding resisted shear stress in cross-section perpendicular to screw axis. Although orientation functions for billet extruded at ER 1.3–4 were equivalent, both strengths changed for the screw extruded at the range of this ER. Infrared spectra suggested transforming from α to β crystal for the billet extruded at the range of this ER. This result suggested that mechanical properties of screw also depended on the crystal forms. 相似文献
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Satoshi?KameokaEmail author Satoru?Wakabayashi Eiji?Abe An?Pang?Tsai 《Catalysis Letters》2016,146(7):1309-1316
115.
Biosynthesis of Trehangelin in Polymorphospora rubra K07–0510: Identification of Metabolic Pathway to Angelyl‐CoA 下载免费PDF全文
Dr. Yuki Inahashi Taro Shiraishi Dr. Kaia Palm Prof. Dr. Yoko Takahashi Prof. Dr. Satoshi Ōmura Prof. Dr. Tomohisa Kuzuyama Dr. Takuji Nakashima 《Chembiochem : a European journal of chemical biology》2016,17(15):1442-1447
Trehangelins are trehalose angelates discovered from endophytic actinomycete Polymorphospora rubra K07‐0510. We identified the trehangelin biosynthetic gene cluster, including genes that encode a glycoside hydrolase‐like protein (thgC), α‐amylase (thgD), 3‐ketoacyl‐ACP synthase III (thgI), 3‐ketoacyl‐ACP reductase (thgK), enoyl‐CoA hydratase (thgH) and acyl transferase (thgJ). Heterologous expression of thgH, thgI, thgJ and thgK confirmed the importance of these genes in the biosynthesis of trehangelin A. Enzymatic activity studies showed that ThgI catalyses the condensation of acetyl‐CoA and methylmalonyl‐CoA to 2‐methylacetoacetyl‐CoA (MAA‐CoA), ThgK catalyses NADPH‐dependent reduction of MAA‐CoA to 3‐hydroxy‐2‐methylbutyryl‐CoA (HMB‐CoA) and ThgH catalyses the dehydration of HMB‐CoA to angelyl‐CoA (AN‐CoA). This is the first report on the elucidation of the enzymatic formation of AN‐CoA. 相似文献
116.
The Role of a Nonribosomal Peptide Synthetase in l‐Lysine Lactamization During Capuramycin Biosynthesis 下载免费PDF全文
Dr. Xiaodong Liu Yuanyuan Jin Zheng Cui Dr. Koichi Nonaka Satoshi Baba Dr. Masanori Funabashi Dr. Zhaoyong Yang Dr. Steven G. Van Lanen 《Chembiochem : a European journal of chemical biology》2016,17(9):804-810
Capuramycins are one of several known classes of natural products that contain an l ‐Lys‐derived l ‐α‐amino‐?‐caprolactam (l ‐ACL) unit. The α‐amino group of l ‐ACL in a capuramycin is linked to an unsaturated hexuronic acid component through an amide bond that was previously shown to originate by an ATP‐independent enzymatic route. With the aid of a combined in vivo and in vitro approach, a predicted tridomain nonribosomal peptide synthetase CapU is functionally characterized here as the ATP‐dependent amide‐bond‐forming catalyst responsible for the biosynthesis of the remaining amide bond present in l ‐ACL. The results are consistent with the adenylation domain of CapU as the essential catalytic component for l ‐Lys activation and thioesterification of the adjacent thiolation domain. However, in contrast to expectations, lactamization does not require any additional domains or proteins and is likely a nonenzymatic event. The results set the stage for examining whether a similar NRPS‐mediated mechanism is employed in the biosynthesis of other l ‐ACL‐containing natural products and, just as intriguingly, how spontaneous lactamization is avoided in the numerous NRPS‐derived peptides that contain an unmodified l ‐Lys residue. 相似文献
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Fracture behaviour and numerical study of resistance spot welded joints in high-strength steel sheet
Teruki Sadasue Satoshi Igi Koichi Taniguchi Rinsei Ikeda Kenji Oi 《Welding International》2016,30(8):602-613
Cross tension tests of resistance spot welded joints with varying nugget diameter were carried out using 980 MPa high strength steel sheet of 1.6 mm thickness. In proportion, as nugget diameter increased from 3√t to 5√t (where t is thickness), cross tension strength (CTS) increased while fracture morphology simultaneously transferred from interface fracture to full plug fracture. In cases of interface fracture, circumferential crack initiation due to separation of the corona bond arose at an early stage of loading. The crack opening process without propagation was recognized until just before fracture and then the crack propagated to the nugget immediately in a brittle manner around CTS. In full plug fracture, main ductile crack initiation from the notch-like part at the end of sheet separation occurred with the sub-crack initiated at an early stage. The ductile crack propagated toward the HAZ and base material to form full plug fracture. The mode I stress intensity factor was considered as a suitable fracture parameter because the circumferential crack behaved pre-crack for brittle fracture in the nugget region at the final stage. Based on the FE analysis, the mode I stress intensity factor was calculated as 116 MPa √m at CTS as fracture toughness for the nugget. With respect to full plug fracture, ductile crack initiation behaviour from the notch-like part was expressed by concentration of equivalent plastic strain. On the assumption that the ductile crack arose in critical value of equivalent plastic strain, the value was calculated as 0.34 by FE analysis. Reasonable interpretation for interface fracture and full plug fracture in the resistance spot welded joint was proposed due to first crack initiation by stress concentration, brittle fracture by using mode I stress intensity factor, and ductile crack initiation by using equivalent plastic strain. 相似文献
119.
Kentaro Fujimoto Yoshiyasu Matsumoto Kohki Oikawa Jun-ichi Nomura Yasuyoshi Shimada Shunrou Fujiwara Kazunori Terasaki Masakazu Kobayashi Kenji Yoshida Kuniaki Ogasawara 《International journal of molecular sciences》2016,17(8)
The purpose of the present study was to determine whether cerebral hyperperfusion after revascularization inhibits development of cerebral ischemic lesions due to artery-to-artery emboli during exposure of the carotid arteries in carotid endarterectomy (CEA). In patients undergoing CEA for internal carotid artery stenosis (≥70%), cerebral blood flow (CBF) was measured using single-photon emission computed tomography (SPECT) before and immediately after CEA. Microembolic signals (MES) were identified using transcranial Doppler during carotid exposure. Diffusion-weighted magnetic resonance imaging (DWI) was performed within 24 h after surgery. Of 32 patients with a combination of reduced cerebrovascular reactivity to acetazolamide on preoperative brain perfusion SPECT and MES during carotid exposure, 14 (44%) showed cerebral hyperperfusion (defined as postoperative CBF increase ≥100% compared with preoperative values), and 16 (50%) developed DWI-characterized postoperative cerebral ischemic lesions. Postoperative cerebral hyperperfusion was significantly associated with the absence of DWI-characterized postoperative cerebral ischemic lesions (95% confidence interval, 0.001–0.179; p = 0.0009). These data suggest that cerebral hyperperfusion after revascularization inhibits development of cerebral ischemic lesions due to artery-to-artery emboli during carotid exposure in CEA, supporting the “impaired clearance of emboli” concept. Blood pressure elevation following carotid declamping would be effective when embolism not accompanied by cerebral hyperperfusion occurs during CEA. 相似文献
120.
Shunzo Kawajiri Takayuki Kawaguchi Yasuharu Watanabe Hiroshi Hayakawa Yasunori Miyamori Dai Nakamura Satoshi Yamashita 《Soils and Foundations》2019,59(3):764-782
For one week from August 17 to 23, 2016, three consecutive typhoons made landfall in Hokkaido for the first time on record. These typhoons and the front they stimulated brought record-breaking torrential rain over the eastern part of Hokkaido. To investigate the damage to grounds and rivers resulting from this rainfall, the Japan Society of Civil Engineers (JSCE) and the Japanese Geotechnical Society (JGS) formed a disaster research group to conduct an investigation. This report provides the results of the investigation into damage to the grounds of areas along the Tokoro River of the Okhotsk region, Hokkaido, that suffered from this tremendous and diverse disaster. Specifically, the report describes the situation of the levees which were broken and eroded by the overflowing water, the shape of the levee bodies, the levee body soil properties examined by observation of the sections, as well as the occurrence of sand boiling and air blows. The washout of road embankments as well as damage to road bridge mounting fills and abutment backfills were also investigated. The investigation has demonstrated the need to clarify the resistance of the abutment backfills and levee bodies to flowing water as well as the geotechnical predominant factors in order to clarify the mechanisms behind erosion and washout, the need to review new measures that allow for the scale of sand boiling and resultant changes in levee body stability, and the fact that the existing embankments were able to temporarily suppress the flooding water which had spilled over from the river. Furthermore, although it has been identified that the findings of a study on an embankment washout associated with a tsunami can be applied to measures taken against the overflowing water, it has also been found necessary to clarify the predominant geotechnical factors using model tests and to use a more sophisticated analytical approach to establish a geotechnical stability review as soon as possible in order to prevent the levees and embankments from being eroded and washed out due to overflowing water. 相似文献