Interaction between chronic arsenic exposure via drinking water and plasma lactate dehydrogenase activity

Arsenic is a potent environmental pollutant that has caused one of the largest public health poisonings in the history of human civilization, affecting tens of millions of people worldwide especially in Bangladesh. Lactate dehydrogenase (LDH) in blood plays an important role in predicting cell or organ damage and as an important clue to the diagnosis of a variety of cancers. However, effect of chronic arsenic exposure on the LDH level in blood has not yet been documented. Since the chronic arsenic exposure is associated with organ damages and multi-site cancers, this research aimed at assaying the plasma level of LDH activity in the population who were exposed to arsenic chronically in Bangladesh. A total of 185 individuals living in arsenic-exposed areas and 121 individuals living in non-exposed area in Bangladesh were recruited as study subjects. Arsenic content in drinking water, hair and nails were estimated by Inductively Coupled Plasma Mass Spectroscopy (ICP-MS) and LDH activity was assayed by a spectrophotometer. Significant increase in LDH activity was observed with increasing concentrations of arsenic in water, hair and nails. Further, the study subjects were split into four groups based on the three ways of each exposure metrics (water, hair and nail arsenic concentrations) where the study subjects in the non-exposed area were used as a reference (lowest exposure) group. LDH activity was found to be increased in the higher exposure groups of water and hair arsenic concentrations. LDH activity was also increased at low to medium exposure groups of nail arsenic concentrations.Thus, the elevated plasma LDH activity might be helpful for the early prognosis of organ or tissue damage in the individuals who were exposed to arsenic chronically.     

A 130-mm2, 256-Mbit NAND flash with shallow trenchisolation technology

A 256-Mbit flash memory has been developed using a NAND cell structure with a shallow trench isolation (STI) process. A tight bit-line pitch of 0.55 μm is achieved with 0.25-μm STI. The memory cell is shrunk to 0.29 μm², which realizes a 130-mm² , 256-Mbit flash memory. Peripheral transistors are scaled with memory cells in order to reduce fabrication process steps. A voltage down converter, which generates 2.5-V constant internal power source, is applied to protect the scaled transistors. An improved bit-line clamp sensing scheme achieves 3.8-μs first access time in spite of long and tight pitch bit-line. A 1-kbyte page mode with 35-ns serial data out realizes 25-Mbyte/s read throughput. An incremental step pulse with a bit by bit verify scheme programs 1-k cells in 1-V Vt distribution within 200 μs. That realizes 4.4-Mbyte/s programming throughput     

A 125-mm/sup 2/ 1-Gb NAND flash memory with 10-MByte/s program speed

A single 3-V only, 1-Gb NAND flash memory has been successfully developed. The chip has been fabricated using 0.13-/spl mu/m CMOS STI technology. The effective cell size including the select transistors is 0.077 /spl mu/m/sup 2/. To decrease the chip size, a new architecture is introduced. The in-series connected memory cells are increased from 16 to 32. Furthermore, as many as 16 k memory cells are connected to the same wordline. As a result, the chip size is decreased by 15%. A very small die size of 125 mm/sup 2/ and an excellent cell area efficiency of 70% are achieved. As for the performance, a very fast programming and serial read are realized. The highest program throughput ever of 10.6-MByte/s is realized: 1) by quadrupling the page size and 2) by newly introducing a write cache. In addition, the garbage collection is accelerated to 9.4-MByte/s. In addition, the write cache accelerates the serial read operation and a very fast 20-MByte/s read throughput is realized.     

Expression of a functional endothelin (ETA) receptor in human meningiomas

Endothelin (ET) receptor subtypes (ETA and ETB) in human meningiomas were characterized using quantitative receptor autoradiography. A single class of high-affinity 125I-ET-1 binding sites was localized in all meningioma tissue studied (dissociation constant: 2.4 +/- 0.3 nM, maximum binding capacity: 319 +/- 66 fmol/mg (mean +/- standard error of the mean for 13 tumors)). Unlabeled ET-1 showed a strong affinity for 125I-ET-1 binding to tissue sections of the tumors with a 50% inhibiting concentration (IC50) of 2.9 +/- 0.7 x 10(-9) M, whereas ET-3 showed a much lower affinity (IC50: 8.4 +/- 2.5 x 10(-6) M). Sarafotoxin S6c, a selective agonist for the ETB receptor, could not compete for 125I-ET-1 binding to meningiomas. Endothelin-1 significantly stimulated deoxyribonucleic acid (DNA) synthesis in a dose-dependent manner in cultured human meningioma cells. In contrast, no significant stimulation of DNA synthesis occurred with an S6c concentration up to 10(-7) M. Pretreatment of the meningioma cells with pertussis toxin, a bacterial toxin that adds adenosine 5'-diphosphate-ribose to the alpha subunit of guanine nucleotide binding (G) proteins such as Gi or G(o), induced a concentration-dependent reduction in ET-stimulated DNA synthesis in meningioma cells, but did not affect the epidermal growth factor-induced DNA synthesis. These observations suggest that the ETA receptor is predominantly expressed in human meningioma tissue and that ET may act as a growth factor on the meningioma cells by interacting with the ETA receptor and by pertussis toxin-sensitive mechanisms.     

A kinetic study of the formation of 12-molybdosilicate and 12-molybdogermanate in aqueous solutions by ion transfer voltammetry with the nitrobenzene-water interface

A novel electrochemical approach was developed for the kinetic study of the formation of heteropolyanions. The method (dual pulse amperometry, DPA) is based on the detections of currents due to the transfers of polyanions at the nitrobenzene-water interface. In this study, DPA was applied to the kinetic study of the formation of two Keggin anions, viz., [SiMo₁₂O₄₀]⁴⁻ and [GeMo₁₂O₄₀]⁴⁻. Prior to the kinetic study, cyclic voltammetric measurements were performed to confirm that the Keggin anion and its lacunary anion ([H₃SiMo₁₁O₃₉]⁵⁻ or [H₃GeMO₁₁O₃₉]⁵⁻) coexist at equilibrium under certain conditions. In DPA, double voltage pulses of different amplitudes were alternately applied to the interface to follow the concentrations of both the Keggin and the lacunary anions. The concentration-time profiles for the polyanions could be elucidated by the two-step consecutive reactions mechanism. The lacunary anion was then found to be the intermediate of the Keggin anion.     

Performance Measure of Hierarchical Structures for Multi-agent Systems

International Journal of Control, Automation and Systems - This paper investigates the robustness of linear consensus networks which are designed under a hierarchical scheme based on Cartesian...     

Complex changes in the framework of endohedrally Na-doped type II Si clathrates with respect to Na content

Crystal structures of endohedrally Na-doped type II silicon clathrates with variable Na content were refined by Rietveld analysis. Type II Si clathrates have two types of cages: small and large. The large cages were preferentially occupied by Na atoms. Upon occupation by Na in the large cages, the lattice constant of the clathrates decreased slightly. The attractive interaction of the Na atoms in the large cages with a framework that caused displacement of the Na atoms from the center might influence lattice shrinkage. Moreover, in the region where almost large cages contained Na atoms and the small cages were partially occupied by Na, the lattice constant increased with the Na content; however, the relationship between these features was complex. The lattice constant demonstrated a linear relationship with the size of the small cages; however, the enlargement of the small cages was not linear with respect to its Na occupancy, resulting in complex changes in the lattice constant with respect to the Na content. It was inferred that enlargement of the small cages by Na insertion may be dependent on the Na occupancy of neighboring small cages.     

Creep rupture behaviour of circumferentially welded mod. 9Cr–1Mo steel pipe subject to internal pressure and axial load

The multi-axial creep strength of circumferential welds in power piping, including failure mode and failure life against a wide range of stress ratios and stress levels, was newly examined in this paper. The creep rupture behaviour of modified 9Cr–1Mo steel (9Cr–1Mo–VNb steel; ASME P91) pipe with a circumferential weld subject to combined internal pressure and axial load was experimentally investigated at 650 °C. The test results, with several kinds of stress ratios of macroscopic axial and hoop stress can be summarised as follows. Along with the increased stress ratio (axial/hoop), both failure location and failure mode changed from a base metal failure caused by hoop stress to an FGHAZ (fine-grained heat affected zone) failure due to axial stress. The stress ratio where the failure mode changed was ‘0.8’. Strength reduction in FGHAZ failure by axial stress should be considered in the structural design of circumferential welds. A series of FEM creep analysis was carried out to discuss the relation of the failure mode to both the local stress distribution and the damage. The failure mode variation along with the increase in the stress ratio is also discussed based on simplified ductile creep failure analysis.     

Activation of restriction enzyme by electrochemically released magnesium ion

Observation and cutting of DNA molecules at intended positions permit several new experimental methods that are completely different from conventional molecular biology methods; therefore several cutting methods have been proposed and studied. In this paper, a new cutting method for a DNA molecule by localizing the activity of a restriction enzyme is presented. Since most restriction enzymes require magnesium ions for their activation, local restriction enzyme activity can be controlled by the local concentration of magnesium ions. Applying a direct current (dc) voltage to a needle electrode of metallic magnesium made it possible to control the local magnesium ion concentration at the tip of the needle. The restriction enzyme was activated only when magnesium ions were electrochemically supplied.     

Motion Generation in MR Environment Using Electrostatic Film Motor for Motion-Triggered Cine-MRI

This paper proposes a new methodology of biomechanical modeling for visualizing tissue deformation, combining an electrostatic film motor and tagged cine-MR imaging. The electrostatic film motor has a simple structure, is easily controlled by open loop, and its MR-compatibility has recently been verified. Tagged cine-MRI is an established process for measuring deformation of active objects. The newly proposed methodology enables the application of cine-MR imaging to passive objects. The electrostatic motor is used to create deformation on passive soft samples inside an MR scanner, the internal deformation of which is visualized by tagged cine-MR imaging. The force applied on the samples is measured by a force sensor fabricated on shielded strain gauges. The impact of the developed system on the MR imaging is verified through SNR evaluation. Then, the proposed methodology is applied to visualizing deformations of a gel sample and a human upper arm to verify the applicability of this methodology in biomechanical measurements.