Magnetic field dependence of the cyclotron effective mass in the organic superconductor κ-(BEDT-TTF)2I3

κ-(BEDT-TTF)₂I₃ is an electronically extreme two-dimensional (2d) organic metal with a superconducting transition at around 4K. In magnetic fields above 12T the effective mass, as obtained from the temperature dependence of the amplitudes of Shubnikov-de Haas (SdH) oscillations, is magnetic field dependent as long as the magnetic field is arranged perpendicular to the conducting planes. In contrast to this by turning the magnetic field 27° or even only by 9° the observed effective mass (as obtained from SdH- and de Haas-van Alphen (dHvA)-measurements) is field independent. We suppose that the occurrence of anyons at temperatures below 1K and in fields above 12T is the reason for the observed field dependence of the effective mass.     

Novel algorithm for time division multiple access in broadband ISDN passive optical networks

For shared access to a broadband communication network using the asynchronous transfer mode (ATM) a passive optical network (PON) has previously been proposed. A novel algorithm to evaluate the time division multiple access (TDMA) protocol which controls the traffic from the various subscribers towards the shared access equipment (upstream direction) is proposed. It uses counters to estimate the moment of a data packet arrival at the subscriber side. By this ‘look-ahead’ technique no extra upstream channels are needed to initiate the polling by the subscriber. The algorithm guarantees fair access for all subscribers and minimizes the necessary overhead. Both the maximum access delay and overhead may be readily calculated, and the obtained results were supported by numerical simulation. Applicability in the standard broadband network environment has been proved by hardware design simulation.     

The radiographic and imaging characteristics of porous tantalum implants within the human cervical spine

STUDY DESIGN: Seven cadaveric cervical spines were implanted with a porous tantalum spacer and a titanium alloy spacer, and their radiographic and imaging characteristics were evaluated. OBJECTIVE: To determine the radiographic characteristics of porous tantalum and titanium implants used as spacers in the cervical spine. SUMMARY OF BACKGROUND DATA: Anterior decompressive surgery of the disc space or the vertebral body creates a defect that frequently is repaired with autologous bone grafts to promote spinal fusion. Donor site morbidity, insufficient donor material, and additional surgical time have spurred the development of biomaterials to replace or supplement existing spinal reconstruction techniques. Although the promotion of a solid bony fusion is critical, the implanted biomaterial should be compatible with modern imaging techniques, should allow visualization of the spinal canal and neural foramina, and should permit radiographic assessment of bony ingrowth. METHODS: Cadaveric spines containing the implants were imaged with plain radiography, computerized tomography, and magnetic resonance imaging. The image distortion produced by the implants was determined qualitatively and quantitatively. RESULTS: The tantalum and titanium spacers were opaque on plain radiographic films. On computed tomographic scans, more streak artifact was associated with the tantalum implants than with the titanium. On magnetic resonance imaging, the porous tantalum implant demonstrated less artifact than did the titanium spacer on T1- and T2-weighted spin echo and on T2*-weighted gradient-echo magnetic resonance images. Overall, the tantalum implant produced less artifact on magnetic resonance imaging than did the titanium spacer and therefore allowed for better visualization of the surrounding bony and neural structures. CONCLUSION: The material properties of titanium and porous tantalum cervical interbody implants contribute to their differential appearance in different imaging methods. The titanium implant appears to image best with computed tomography, whereas the porous tantalum implant produces less artifact than does the titanium implant on several magnetic resonance imaging sequences.     

Isolation and stability of histidine-tagged proteins produced in plants via potyvirus gene vectors

A system for the expression and purification of histidine-tagged proteins from plants has been developed using a tobacco etch potyvirus (TEV)-derived gene vectors. The vectors offered a convenient polylinker and a choice of histidine tagging at the recombinant proteins' N or C termini. These vectors were utilized for expression of proteins encoded by beet yellows closterovirus (BYV). Approximately 4 micrograms/g of 20-kDa BYV protein was readily isolated from plants systemically infected by hybrid TEV. In contrast, only minute quantities of 22-kDa BYV capsid protein (CP) histidine-tagged at its N or C terminus could be purified. Rapid degradation of the recombinant CP has been implicated in its failure to accumulate in infected plants. Fusion with TEV HC-Pro stabilized the histidine-tagged BYV CP and facilitated purification of the fusion product from infected plants. This same fusion approach was successfully used with the 24-kDa minor BYV CP. The recombinant proteins were recognized by histidine-tag-specific monoclonal antibody in immunoblot analysis. These results demonstrate the utility of a designed series of TEV vectors for expression, detection, and purification of the recombinant proteins and suggest that intrinsic protein stability is a major factor in a recovery of recombinant proteins from plants.     

Identification of an insulin-responsive, slow endocytic recycling mechanism in Chinese hamster ovary cells

In adipocytes, the insulin-regulated aminopeptidase (IRAP) is trafficked through the same insulin-regulated recycling pathway as the GLUT4 glucose transporter. We find that a chimera, containing the cytoplasmic domain of IRAP fused to transmembrane and extracellular domains of the transferrin receptor, is slowly recycled and rapidly internalized in Chinese hamster ovary cells. Morphological studies indicate that the chimera is slowly trafficked through the general endosomal recycling compartment rather than being sorted to a specialized recycling pathway. A chimera in which a di-leucine sequence within the cytoplasmic domain of IRAP has been mutated to alanines is rapidly internalized and rapidly recycled, indicating that this di-leucine is required for the slow recycling but not for the rapid internalization. Insulin stimulates a 2-3-fold increase in the recycling of the chimera and only a 1.2-fold increase in the recycling of the transferrin receptor. The effect of insulin on the recycling of the chimera is blocked by wortmannin, a phosphatidylinositol 3'-kinase inhibitor. GTPgammaS (guanosine 5'-3-O-(thio)triphosphate) increases the recycling of the chimera by 50% but has no effect on the recycling of the transferrin receptor. In these studies, we have identified in Chinese hamster ovary cells a novel, slow endocytic recycling mechanism that is regulated by insulin.     

A study on the orientation effects in polyethylene in the light of crystalline texture

Special sample types established and characterised in Parts 1 and 2 [1,2] were examined systematically under different modes of external deformation at constant temperatures. The structural changes recorded by means of wide- and low-angle X-ray patterns fully substantiated the bodily rotation of the otherwise unaltered crystallites in accordance with the interlamellar slip mechanism postulated in Parts 1 and 2, both in tension and under two modes of compression. Dimensional changes, however, were significantly in excess of those expected from the rotation of crystallites, but were uniquely correlated with this relation in the different samples under different conditions of deformation. This points to a unique structural and mechanistic interrelation between the crystallites and more compliant amorphous regions. As the X-ray long periods were unaffected by the deformation, the more extensible material cannot be identical to that associated with the lamellar periodicity, a fact which points to a previously unsuspected superlamellar regularity in the texture. A simple structural suggestion is made.     

Intracondylar knee joint prosthesis with rotation capacity- Endo model

The St. Georg total knee prosthesis has been modified to incorporate axial rotation of the tibial component. The kinematics approximate more those of the normal knee and the stress imposed at the bone-cement interface are attenuated, thus reducing the risk of mechanical failure. The construction and laboratory testing are described.