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In this study 1,5-glucono-δ-lactone was used to achieve acid-induced gelation of myofibrillar proteins at 4 °C. The characteristics of the myofibrillar gels were investigated by measuring Young's modulus and springiness. The effects of addition of sodium chloride and tetrasodium pyrophosphate on gel characteristics were also studied. Sodium chloride increased the Young's modulus of myofibrillar protein gels in the presence and absence of 1,5-glucono-δ-lactone, while tetrasodium pyrophosphate had no observable effect on the gels until the pH decreased to 4.1, when the Young's modulus was enhanced. 相似文献
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Vukovic A Sewell P McKirdy D Thomas D Benson TM Christopoulos C Glover P 《IEEE transactions on medical imaging》2008,27(6):766-774
This paper describes a dynamic vector model for modelling the electromagnetic characteristics of microstrip radio-frequency (RF) resonators for high field magnetic resonance imaging (MRI). A biological tissue-equivalent load having a circular cross section is assumed in the analysis. The dynamic model uses the well-known Green's function for cylindrically stratified media to characterize all six components of the electromagnetic field excited by the microstrip lines. The accuracy of the method as a function of its parameters is assessed and the results compared with those obtained from the quasi-static method often used at low frequencies. The limits of the quasi-static assumption are investigated by comparing values for the modal propagation constant and the terminating capacitances required to tune the cavity resonance over a frequency range of 100 MHz-1 GHz. The dynamic method is further used to analyse the modal content of a microstrip head resonator. Finally, a variational approach is used to assess the impact of the intermodal coupling for the case of small perturbations in the shape and the position of the cylindrical phantom. 相似文献
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Corn, oats, molasses, and soyhulls are commonly used carbohydrate sources in calf starters. A total of 180 calves were used in 4 studies to compare the use of these ingredients in calf starters. Study 1 compared textured starters with different amounts of molasses or sucrose. The control starter contained 5% molasses (A). The test starters contained greater concentrations of dietary sugar than starter A as either 10% molasses (B) or 5% molasses plus 1.5% granular sucrose (C). Starters B and C were equal in dietary sugar. Study 2 evaluated textured starters containing 0 or 25% whole oats for calves up to approximately 12 wk old. Study 3 evaluated pelleted starters containing 0 or 62.75% soyhulls for calves up to approximately 8 wk old. Study 4 evaluated textured starters containing 0, 14, 28, and 42% soyhulls for calves between approximately 8 and 12 wk old. Calves were housed in individual pens in an unheated nursery with curtain sides through 8 wk and then in group pens of 6 calves/pen from 8 to 12 wk. Calves were bedded with straw. In study 1, calves fed the starters with extra molasses or sucrose had an average of 9% slower average daily gain (ADG) and greater average fecal scores from 42 to 56 d and 9% slower ADG from 0 to 56 d than calves fed the textured starter with low molasses. In study 2, ADG and feed efficiency (kg of feed/kg of gain) were 22 and 20% less, respectively, in calves fed the starter without oats from 0 to 28 d, but there were no differences thereafter. In study 3, calves fed starters with soyhulls had a 10% slower ADG and 8% lower efficiency of gain from 28 to 56 d than calves fed the starters without soyhulls. In study 4, ADG declined linearly as soyhulls increased in the starter. The change in ADG was 14% from 0 to 42% soyhulls. Replacing corn in a starter with molasses, sucrose, or soyhulls reduced postweaning ADG and increased the cost of ADG. Whole oats were an acceptable substitute for corn. 相似文献
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This investigation compared electrophoretic profiles of exudate from fresh normal pork in which the protein separation was undertaken by both sodium dodecyl sulphate polyacrylamide gel electrophoresis SDS PAGE and capillary gel electrophoresis. The profiles obtained using the two techniques were similar and in many investigations either method would be suitable. It is suggested that capillary gel electrophoresis offers a number of benefits over the traditional time consuming and labour intensive gel electrophoresis techniques which involves gel preparation, sample application, staining and eventually quantification of the zones by densitometry. For separation of proteins in meat-based systems the advantages of capillary gel electrophoresis include on-capillary detection, instrumental automation, rapid analysis time and low sample volumes while providing similar information to that obtained from SDS PAGE. 相似文献
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Optimal concentrations of lysine, methionine, and threonine in milk replacers for calves less than five weeks of age 总被引:1,自引:0,他引:1
Hill TM Bateman HG Aldrich JM Schlotterbeck RL Tanan KG 《Journal of dairy science》2008,91(6):2433-2442
The AA requirements of herd-replacement calves less than 5 wk old and fed milk replacers are not clearly defined and have been estimated in a limited number of studies using milk-fed calves ranging from 5 to 20 wk of age. The objective of these 4 studies was to investigate the effect of supplementing milk replacers containing 24 to 28% crude protein (CP; from milk sources) and 17% fat with Lys, Met, and Thr to estimate the optimum requirements for calves less than 5 wk of age. Holstein bull calves (initially 3 and 4 d old, 43 ± 1 kg of body weight, BW) were fed an 18% CP (as-fed) starter ad libitum and weaned at 31 to 32 d of age (28-d studies). Calves were housed in an unheated, curtain-sided nursery. In study 1, 6 milk replacer treatments were fed based on the combination of 3 CP concentrations (24, 26, and 28% CP) each with or without added Lys and Met. In studies 2 and 3, 26% CP and 2.34% Lys milk replacer treatments were fed to test the concentration of Met (0.64, 0.68, and 0.72% Met in study 2 and 0.64, 0.72, and 0.80% Met in study 3). In study 4, 26% CP, 2.34% Lys, and 0.72% Met milk replacer treatments were fed to test the concentration of Thr (1.06, 1.43, and 1.80%). There was a 17% improvement in average daily gain (ADG) in study 1 from adding Lys and Met that was maximized with 2.34% Lys. The ADG response to added Met in studies 2 (linear) and 3 (quadratic) were 13 and 7%, respectively, with a plateau at 0.72% Met. There was no ADG or efficiency response to added Thr in study 4. Formulating 17% fat, whey-based milk replacers fed at 0.68 kg/d to 26% CP, 2.34% Lys, and 0.72% Met appeared optimum based on responses of body weight gain, feed efficiency, and serum concentrations of urea nitrogen, while feeding calves more CP and essential AA did not improved ADG and efficiency. Requirements for calves less than 5 wk old, averaging 48 kg of BW, consuming 204 g of CP/d, and gaining 0.46 kg of BW/d, appeared to be met with 17 g of Lys, 0.31 Met:Lys ratio, 0.54 Met+Cys:Lys ratio, and a Thr:Lys ratio less than 0.60. 相似文献
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The influence of natural organic matter rigidity on the sorption, desorption, and competitive displacement rates of 1,2-dichlorobenzene 总被引:1,自引:0,他引:1
The influence of natural organic matter (NOM) rigidity on the sorption, desorption, and competitive displacement rates of 1,2-Dichlorobenzene (1,2-DCB) was evaluated using batch reactor experiments with two surface soils (Yolo and Forbes) and a shale (Ohio). Previous characterization suggests that the shale NOM is the most reduced and condensed, the Yolo soil is the most oxidized and amorphous, and Forbes soil has an intermediate NOM structure. The rate study for each sorbent was conducted under the same reactor parameters, and 1,2-DCB mass-transfer rates were determined using the distributed first-order mass-transfer rate model based on the gamma probability density function. To measure competitive displacement rates, 1,2,4-trichlorobenzene (1,2,4-TCB) was delivered as a competitor after 34 days pre-equilibration. Higher fractions of contaminant subject to instantaneous mass transfer and much faster rates of approach to apparent sorption equilibrium are found in Yolo soil when compared with Forbes soil and the shale. The size of the instantaneously desorbing fraction thus appears inversely related to the hard carbon fraction. In the NOM compartment where mass transfer is rate-limited, rate coefficient distributions are shifted toward lower rates for desorption and competitive displacement of 1,2-DCB in Ohio shale, followed by Forbes soil. Sorption and desorption rate distributions are almost the same for the shale, while desorption rates are a few times greater than sorption rates in Yolo and Forbes soils. Mass-transfer coefficients for competitive displacement are considerably slower than those for desorption in Forbes soil and the shale. However, the mass-transfer rates for the two processes seem to be similar in Yolo soil, which has a NOM matrix comprising a relatively larger soft organic carbon fraction. The concept of "solute induced softening" is discussed as a mechanistic rationale for the experimental observations. 相似文献