全文获取类型
收费全文 | 636篇 |
免费 | 27篇 |
国内免费 | 3篇 |
专业分类
电工技术 | 22篇 |
综合类 | 1篇 |
化学工业 | 208篇 |
金属工艺 | 9篇 |
机械仪表 | 19篇 |
建筑科学 | 8篇 |
能源动力 | 22篇 |
轻工业 | 64篇 |
无线电 | 58篇 |
一般工业技术 | 137篇 |
冶金工业 | 36篇 |
原子能技术 | 30篇 |
自动化技术 | 52篇 |
出版年
2023年 | 8篇 |
2022年 | 10篇 |
2021年 | 17篇 |
2020年 | 5篇 |
2019年 | 2篇 |
2018年 | 24篇 |
2017年 | 11篇 |
2016年 | 10篇 |
2015年 | 10篇 |
2014年 | 24篇 |
2013年 | 35篇 |
2012年 | 24篇 |
2011年 | 26篇 |
2010年 | 25篇 |
2009年 | 25篇 |
2008年 | 29篇 |
2007年 | 21篇 |
2006年 | 21篇 |
2005年 | 26篇 |
2004年 | 20篇 |
2003年 | 35篇 |
2002年 | 31篇 |
2001年 | 13篇 |
2000年 | 14篇 |
1999年 | 13篇 |
1998年 | 18篇 |
1997年 | 22篇 |
1996年 | 17篇 |
1995年 | 14篇 |
1994年 | 13篇 |
1993年 | 12篇 |
1992年 | 9篇 |
1991年 | 4篇 |
1990年 | 6篇 |
1989年 | 9篇 |
1988年 | 7篇 |
1987年 | 6篇 |
1986年 | 7篇 |
1984年 | 5篇 |
1983年 | 7篇 |
1982年 | 5篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1978年 | 6篇 |
1977年 | 1篇 |
1976年 | 3篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 1篇 |
1970年 | 1篇 |
排序方式: 共有666条查询结果,搜索用时 15 毫秒
51.
Hirofumi Kakemoto Tohru Higuchi Hajime Shibata Satoshi Wada Takaaki Tsurumi 《Journal of Materials Science: Materials in Electronics》2008,19(4):311-314
The modification of thermoelectric figure of merit was estimated from enhanced mobility of [100] oriented beta-FeSi2 film. beta-FeSi2 on Si(001) substrate was prepared by molecular beam epitaxy method using an Fe source. The crystallographic orientation of
beta-FeSi2 film on Si(001) substrate was characterized by using X-ray diffraction. Using scanning electron microscopy, surface morphology
and film thickness of samples were observed and estimated, respectively. The mobility of beta-FeS2 film on Si(001) substrate were also characterized by Hall measurement at room temperature. A part of the enhancement of figure
of merit was evaluated as the functions of mobility and crystallographic orientation of samples. 相似文献
52.
An agarolytic bacterium was isolated from soil in Gifu prefecture, Japan, and identified as Bacillus sp. strain MK03. The strain secreted neoagarooligosaccharide hydroluse into the culture medium. The enzyme was purified 49.7-fold from the culture fluid by ammonium sulfate precipitation and anion-exchange and gel-filtration column chromatographic methods. The purified enzyme appeared as a single band on polyacrylamide gel electrophoresis (PAGE) and sodium dodecyl sulfate (SDS)-PAGE. Estimations of the molecular mass by gel filtration and SDS-PAGE gave values of 320 kDa and 42 kDa, respectively, indicating that the enzyme is octametric. The enzyme cleaved the alpha-1,3 linkage in neoagarobiose to produce 3,6-anhydro-L-galactose and D-galactose. It also selectively cleaved the alpha-1,3 linkage at the nonreducing end in neoagarotetraose or neoagarohexaose to give 3,6-anhydro-L-galactose and agarotriose or agaropentaose. The optimum temperature and pH for the enzyme were 30 degrees C and 6.1, respectively. The N-terminal amino acid sequence showed no homology to sequences of other known neoagarooligosaccharide hydrolases and agarases. 相似文献
53.
54.
55.
A monolayer of dodecanethiol-stabilized gold nanoparticles changed into two-dimensional and three-dimensional self-organized structures by annealing at 323 K. Subsequent crystal growth of gold nanoparticles occurred. Thiol molecules, although chemisorbed, form relatively unstable bonds with the gold surface; a few thiols desorbed from the surface and oxidized to disulfides at 323 K, because the interaction energy between thiol macromolecules is larger than that between a thiol and a nanoparticle. The gold nanoparticles approached each other and grew into large single or twinned crystals because of the van der Waals attraction and the heat generated by the exothermic formation of disulfides. 相似文献
56.
Tohru Sugiyama Yoshiaki Kudo Youichi Takayama 《Journal of the Society for Information Display》2009,17(1):29-35
Abstract— Soft proofing, which can confirm the color reproduction of printed matter on a monitor, is coming into wide use in the field of graphic arts. However, there is a problem in that the color on the monitor looks different from that of printed matter, even though the L*a*b* value of the monitor's white point has been adjusted to that of the paper by using a spectroradiometer. After the color rendition of an LCD is visually adjusted to that of the paper, the measured color of the LCD shows color with L*a*b* values corresponding to a more greenish‐blue white than that of paper. For CRTs, this corresponds to a more bluish‐white. In this paper, it was assumed that bright lines in the measured spectrums of the monitors and the illuminations spread to the next wavelength band by the optical systems of the spectroradiometer. To solve the problem, a method is proposed to enhance the bright line by using a three‐tap digital filter. The effect of this method on two types of monitors under three types of illumination is also reported. After enhancing the bright lines, ΔE between the monitor and paper becomes smaller than that for the original one. 相似文献
57.
T Yoshio JI Masuyama N Kohda D Hirata H Sato M Iwamoto A Mimori A Takeda S Minota S Kano 《Canadian Metallurgical Quarterly》1997,24(3):489-495
OBJECTIVE: To investigate how sera from 37 patients with systemic lupus erythematosus (SLE) stimulate interleukin (IL) 6 release from IL-1beta pretreated endothelial cells and compare these effects to those of sera from 16 normal controls. METHODS: Endothelial cells pretreated 18 h with IL-1beta (5 U/ml) were incubated 2 h with sera diluted 10-fold with phosphate buffered saline (PBS). IL-6 concentrations in endothelial culture supernatants collected after incubation were measured by ELISA. RESULTS: Compared with PBS, sera from controls and 24 patients with SLE suppressed IL-6 release from IL-1beta pretreated cells. However, sera from 13 patients with SLE augmented IL-6 release. Of note, sera from 5 patients with pulmonary hypertension induced the highest level of IL-6 release. IgG from control sera suppressed IL-6 release, whereas F(ab')2 did not. Both IgG and F(ab')2 from the sera of patients with SLE with pulmonary hypertension augmented IL-6 release from IL-1beta pretreated cells. CONCLUSION: IgG antiendothelial cell antibodies from patients with SLE may be associated with the pathogenesis of SLE and pulmonary hypertension. 相似文献
58.
Rapid Reconstitution of Biosynthetic Machinery for Fungal Metabolites in Aspergillus oryzae: Total Biosynthesis of Aflatrem 下载免费PDF全文
Koichi Tagami Dr. Atsushi Minami Ryuya Fujii Dr. Chengwei Liu Dr. Mizuki Tanaka Prof. Dr. Katsuya Gomi Prof. Dr. Tohru Dairi Prof. Dr. Hideaki Oikawa 《Chembiochem : a European journal of chemical biology》2014,15(14):2076-2080
Reconstitution of the biosynthetic machinery for fungal secondary metabolites in Aspergillus oryzae provides an opportunity both for stepwise determination of the biosynthetic pathways and the total biosynthesis of fungal natural products. However, to maximize the utility of the reconstitution system, a simple and rapid strategy for the introduction of heterologous genes into A. oryzae is required. In this study, we demonstrated an effective method for introducing multiple genes involved in the biosynthesis of fungal metabolites by using the expression vectors pUARA2 and pUSA2, each of which contains two cloning sites. The successful introduction of all the aflatrem biosynthetic genes (seven genes in total) after two rounds of transformation enabled the total biosynthesis of aflatrem. This rapid reconstitution strategy will facilitate the functional analysis of the biosynthetic machinery of fungal metabolites. 相似文献
59.
The tensile fatigue behaviour of ultra-high performance fibre reinforced concrete (UHPFRC) under constant amplitude fatigue cycles is presented. Three series of uniaxial tensile fatigue tests up to a maximum of 10 million cycles were conducted with the objective to determine the endurance limit of UHPFRC that was supposed to exist for this material. The fatigue tests reveal that an endurance limit exists in all three domains of UHPFRC tensile behaviour at S-ratios ranging from 0.70 to 0.45 with S being the ratio of the maximum fatigue stress to the elastic limit strength of UHPFRC. Rather large variation in local specimen deformations indicates significant stress and deformation redistribution capacity of the UHPFRC bulk material enhancing the fatigue behaviour. The fatigue fracture surface of UHPFRC shows features of the fatigue fracture surfaces of steel, i.e. fatigue crack propagation is identified by a smooth surface while final fracture leads to rather rough surface. Various fatigue damaging mechanisms due to fretting and grinding as well as tribocorrosion are identified. 相似文献
60.