Formulation,optimization, hemocompatibility and pharmacokinetic evaluation of PLGA nanoparticles containing paclitaxel

Objective: Paclitaxel (PTX)-loaded polymer (Poly(lactic-co-glycolic acid), PLGA)-based nanoformulation was developed with the objective of formulating cremophor EL-free nanoformulation intended for intravenous use.

Significance: The polymeric PTX nanoparticles free from the cremophor EL will help in eliminating the shortcomings of the existing delivery system as cremophor EL causes serious allergic reactions to the subjects after intravenous use.

Methods and results: Paclitaxel-loaded nanoparticles were formulated by nanoprecipitation method. The diminutive nanoparticles (143.2?nm) with uniform size throughout (polydispersity index, 0.115) and high entrapment efficiency (95.34%) were obtained by employing the Box–Behnken design for the optimization of the formulation with the aid of desirability approach-based numerical optimization technique. Optimized levels for each factor viz. polymer concentration (X1), amount of organic solvent (X2), and surfactant concentration (X3) were 0.23%, 5?ml %, and 1.13%, respectively. The results of the hemocompatibility studies confirmed the safety of PLGA-based nanoparticles for intravenous administration. Pharmacokinetic evaluations confirmed the longer retention of PTX in systemic circulation.

Conclusion: In a nutshell, the developed polymeric nanoparticle formulation of PTX precludes the inadequacy of existing PTX formulation and can be considered as superior alternative carrier system of the same.     

Preparation,characterization, and in vivo evaluation of nano formulations of ferulic acid in diabetic wound healing

Diabetes mellitus is most common disorder characterize by hyperglycemia. Chronic hyperglycemia may lead to over production of free radicals thereby results in oxidative stress which impaired healing of wounds. Ferulic acid (FA) has been shown to have antidiabetic and antioxidant properties. The aim of the present study was to develop Ferulic acid nanoparticles and to study its hypoglycemic and wound healing activities. Ferulic acid-poly(lactic-co-glycolic acid) (FA-PLGA) nanoparticles were prepared by nano precipitation method. The prepared FA-PLGA nanoparticles had an average size of 240?nm. Scanning electron microscopy (SEM) and atomic force microscopy (AFM) analysis showed the prepared FA-PLGA nanoparticles were spherical in shape. Drug encapsulation assay showed that 88.49% FA was encapsulated in PLGA. Carbopol 980 was used to formulate FA-PLGA nanoparticles loaded hydrogel. FA-loaded polymeric nanoparticles dispersion (oral administration) and FA-loaded polymeric nanoparticles based hydrogel (topical administration) treated wounds were found to epithelize faster as compared with diabetic wound control group. The hydroxyproline content increased significantly when compared with diabetic wound control. Therefore, the results indicate that FA significantly promotes wound healing in diabetic rats.     

Residual U-Network for Breast Tumor Segmentation from Magnetic Resonance Images

Breast cancer positions as the most well-known threat and the main source of malignant growth-related morbidity and mortality throughout the world. It is apical of all new cancer incidences analyzed among females. Two features substantially influence the classification accuracy of malignancy and benignity in automated cancer diagnostics. These are the precision of tumor segmentation and appropriateness of extracted attributes required for the diagnosis. In this research, the authors have proposed a ResU-Net (Residual U-Network) model for breast tumor segmentation. The proposed methodology renders augmented, and precise identification of tumor regions and produces accurate breast tumor segmentation in contrast-enhanced MR images. Furthermore, the proposed framework also encompasses the residual network technique, which subsequently enhances the performance and displays the improved training process. Over and above, the performance of ResU-Net has experimentally been analyzed with conventional U-Net, FCN8, FCN32. Algorithm performance is evaluated in the form of dice coefficient and MIoU (Mean Intersection of Union), accuracy, loss, sensitivity, specificity, F1score. Experimental results show that ResU-Net achieved validation accuracy & dice coefficient value of 73.22% & 85.32% respectively on the Rider Breast MRI dataset and outperformed as compared to the other algorithms used in experimentation.     

Miniaturized Microstrip Patch Antenna Array for ISM Band Using Complementary Split Ring Resonator

Wireless Personal Communications - These days patch antenna arrays are widely used in various communication systems. In this research design of miniaturized microstrip patch antenna array is...     

Trust-aware energy-efficient stable clustering approach using fuzzy type-2 Cuckoo search optimization algorithm for wireless sensor networks

Wireless Networks - With the advancement of communication and sensor technologies, it has become possible to develop low-cost circuitry to sense and transmit the state of surroundings. Wireless...     

Relationship of Water Stress and Flood Damage for Sustainable Development

Water Resources Management - Climate change leads to the extreme occurrence of events, water-related disasters, and pressure on existing water resources. The water resource is important for the...     

Multiband Multiple Elliptical Microstrip Patch Antenna with Circular Polarization

In this paper a compact low profile elliptical fractal patch antenna for high gain and multiband is presented which resembles Lorentz fractal shape. By introducing fractal on ellipse patch multiband applications are achieved. With the help of CST Microwave Studio Suite TM the proposed structure has been designed and analyzed. The simulated results are confirmed experimentally. The suggested antenna has a dimension of 40?×?55 mm² (L?×?W) and resonant frequencies obtained are 1.7, 3.2, 3.9, 5.9, 7.8, 8.6, 9.7, 11.4 and 12.6 GHz with VSWR?≤?2. The aerial is assembled on FR-4 (ε_r?=?4.4) substrate with thickness of substrate 1.6 mm. This antenna holds applications in many satellite communication transmissions, some Wi-Fi devices, cordless telephones, and weather radar systems.     

A BER Based Selection Combining Protocol for Adaptive Cooperative Cognitive Radios

This paper illustrates the performance of bit error rate based selection combining (BER-SC) protocol for adaptive cooperative cognitive radios. In the proposed framework, the unlicensed (i.e. secondary) system utilizes an adaptive mode of transmission to help the licensed (i.e. primary) system to achieve the desired quality of service in exchange for opportunistic spectrum access. The total transmission is divided in two phases. In Phase I, the primary transmitter (PT) broadcasts the data to the primary receiver (PR), which is overheard by the secondary transmitter (ST) and secondary receiver (SR). In Phase II, ST decodes the primary data and linearly combines its own data with the primary data. Using M-QAM the combined data is adaptively modulated, where M = 4, 16 or 64 depending on the received channel feedback, and relayed to PR and SR. At PR, BER-SC is employed to retrieve the primary data, and at SR interference cancellation is used to retrieve the secondary data. The analytical expressions are derived for the BER and the outage probability. The obtained results demonstrate the higher performance gains for both primary and secondary system by using adaptive mode of transmission at ST and BER-SC at PR.

     

A stable energy efficient clustering protocol for wireless sensor networks

Sensor networks comprise of sensor nodes with limited battery power that are deployed at different geographical locations to monitor physical events. Information gathering is a typical but an important operation in many applications of wireless sensor networks (WSNs). It is necessary to operate the sensor network for longer period of time in an energy efficient manner for gathering information. One of the popular WSN protocol, named low energy adaptive clustering hierarchy (LEACH) and its variants, aim to prolong the network lifetime using energy efficient clustering approach. These protocols increase the network lifetime at the expense of reduced stability period (the time span before the first node dies). The reduction in stability period is because of the high energy variance of nodes. Stability period is an essential aspect to preserve coverage properties of the network. Higher is the stability period, more reliable is the network. Higher energy variance of nodes leads to load unbalancing among nodes and therefore lowers the stability period. Hence, it is perpetually attractive to design clustering algorithms that provides higher stability, lower energy variance and are energy efficient. In this paper to overcome the shortcomings of existing clustering protocols, a protocol named stable energy efficient clustering protocol is proposed. It balances the load among nodes using energy-aware heuristics and hence ensures higher stability period. The results demonstrate that the proposed protocol significantly outperforms LEACH and its variants in terms of energy variance and stability period.     

Potentiometric studies of N,N′-Bis(2-dimethylaminoethyl)-N,N′-dimethyl-9,10 anthracenedimethanamine as a chemical sensing material for Zn(II) ions

A liquid membrane based Zn²⁺ ion selective electrode containing N,N′-Bis(2-dimethylaminoethyl)-N,N′-dimethyl-9,10 anthracenedimethanamine (Bis(TMEDA) anthracene) (I) as ionophore has been prepared and characterized. The membrane comprises of PVC, ionophore and plasticizer in the ratio of 33:2:65, respectively. It showed the best response in terms of detection limit (1.5 × 10^− 6 M) and working concentration range (1.0 × 10^− 5 M to 1.0 × 10^− 1 M) with Nernstian response towards Zn²⁺ ions. The electrode responds within 15 s of coming in contact with the solution. The potential response remains almost unchanged over a pH range of 3.0 to 7.5. The electrode can be used for at least 3 months without any considerable alteration in its response behavior. The proposed electrode revealed good selectivity towards Zn²⁺ ions over a number of alkali, alkaline earth, transition metals and some other heavy metal ions. The electrode has been used as an indicator electrode in the potentiometric titration of Zn²⁺ with EDTA. The proposed electrode also detected Zn²⁺ ions from real life samples.