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71.
In the last decade the 'comet assay' or 'single cell gel electrophoresis assay' has been established as a sensitive method for the detection of DNA damage and the measurement of its recovery. The results published in the literature have often been obtained with different methods for comet structure measurement. In most cases these data are not comparable with each other. Even when using similar systems for the analysis, it is difficult to obtain matching data. This presentation will describe some technical aspects of our measurement equipment and evaluation software. It focuses on necessary experimental conditions to minimize errors in obtaining such data. The software developed here allows the rapid analysis of the microscopic samples (< 2 s per image). The image analysis was designed with respect to the morphological shapes of comet cells, which were investigated with a confocal laser microscope. The system is built with standard components which are commercially available. As a measure of the amount of DNA damage the ratio of fluorescence intensity was used inside the comet tail and the fluorescence intensity of the comet head. Other parameters such as DNA content, comet area, head radius, tail length and tail moment are also determined. The reproducibility of the system has been evaluated in several experiments over a period of 5 years. 相似文献
72.
The microstructure development of Pd77.5Au6Si16.5 alloy droplet solidified in a drop tube process was studied. It was found that two distinct microstructures, i.e. (Pd,Au)3Si primary phase and Pd+(Pd,Au)3Si eutectic can be obtained when the droplet diameter is within the range between 2.3~0.4 mm. The morpologies of the (Pd,Au)3Si developed from dendrite trunk-like with single branching only into dendrite cluster-like with ternary branching with the decrease of the droplet diameter. When the droplet diameter is about 0.25 mm, the primary phase (Pd,Au)3Si almost disappears and the microstructure mainly shows Pd+(Pd,Au)3Si eutectic. The morphology of the eutectic transforms from fiber-like to plate-like with the decrease of the droplet diameter in the range between 2.3-0.25 mm. When the droplet diameter is about 0.19 mm, the microstructure is only the single phase of Pd solid solution 相似文献
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The reduced ascorbic acid and β-carotene contents of “retail market-fresh” (RMF) and “fresh-frozen” (FF) green beans and broccoli in a simulated handling system were determined. Changes in ascorbic acid content in the two vegetables during a retail market period and frozen storage followed different patterns. In green beans, ascorbic acid content decreased during refrigerated storage for up to 7 days, but in broccoli there was a significant increase. Blanching resulted in a loss of approximately 40% of the ascorbic acid in broccoli. Ascorbic acid content of FF green beans stored at-20C for 16 weeks was approximately twice that in RMF green beans, but in FF broccoli stored under the same conditions it was only about half that of RMF broccoli. β-carotene content of green beans and broccoli did not change during either the retail market simulation of frozen storage and did not differ from that of fresh. 相似文献
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低碳微合金钢中的晶内铁素体及组织控制 总被引:1,自引:0,他引:1
回顾和评述了晶内铁素体的发现、发展及其与组织控制的关系.介绍了晶内铁素体的形核机制和影响晶内铁素体形成的因素,讨论了晶内铁素体的三维形态和长大动力学,并从以上几方面分析了晶内铁素体对低碳微合金钢组织细化的重要作用和影响. 相似文献
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辽河油田东部凹陷火山岩油藏较为集中分布.然而常规酸化效果较差.经采用三元酸(粉末硝酸、盐酸和氢氟酸)前置液压裂酸化后.获得显著的增产效果,进一步表明该项酸化工艺技术成熟,宜于推广应用。同时也面临技术集成再创新机遇.适应更广泛的地质需求。 相似文献
80.
兰州石化公司以加工新疆原油为主,重油收率高,平均在37%左右,所生产的重油主要靠催化裂化装置来加工,多余部分以低附加值重油出厂。随着涩宁兰天然气工程的逐渐建成,低附加值重油出厂将受到严重威胁,因此,催化裂化装置加工重油显得尤为重要。兰州石化公司炼油厂1.2Mt/a重催装置通过采用先进技术进行改造,使重油掺炼比由41.52%提高到52.20%,提高了10.68个百分点,年多加工重油14.95万t,产品收率达到79.20%以上,能够灵活调节产品结构,创造了良好的经济效益。 相似文献