Application potential of bone marrow mesenchymal stem cell (BMSCs) based tissue-engineering for spinal cord defect repair in rat fetuses with spina bifida aperta

Spina bifida aperta are complex congenital malformations resulting from failure of fusion in the spinal neural tube during embryogenesis. Despite surgical repair of the defect, most patients who survive with spina bifida aperta have a multiple system handicap due to neuron deficiency of the defective spinal cord. Tissue engineering has emerged as a novel treatment for replacement of lost tissue. This study evaluated the prenatal surgical approach of transplanting a chitosan–gelatin scaffold seeded with bone marrow mesenchymal stem cells (BMSCs) in the healing the defective spinal cord of rat fetuses with retinoic acid induced spina bifida aperta. Scaffold characterisation revealed the porous structure, organic and amorphous content. This biomaterial promoted the adhesion, spreading and in vitro viability of the BMSCs. After transplantation of the scaffold combined with BMSCs, the defective region of spinal cord in rat fetuses with spina bifida aperta at E20 decreased obviously under stereomicroscopy, and the skin defect almost closed in many fetuses. The transplanted BMSCs in chitosan–gelatin scaffold survived, grew and expressed markers of neural stem cells and neurons in the defective spinal cord. In addition, the biomaterial presented high biocompatibility and slow biodegradation in vivo. In conclusion, prenatal transplantation of the scaffold combined with BMSCs could treat spinal cord defect in fetuses with spina bifida aperta by the regeneration of neurons and repairmen of defective region.     

Porous nano-hydroxyapatite/collagen scaffold containing drug-loaded ADM–PLGA microspheres for bone cancer treatment

To develop adriamycin (ADM)-encapsulated poly(lactic-co-glycolic acid) (PLGA) nanoparticles in a porous nano-hydroxyapatite/collagen scaffold (ADM–PLGA–NHAC). To provide novel strategies for future treatment of osteosarcoma, the properties of the scaffold, including its in vitro extended-release properties, the inhibition effects of ADM–PLGA–NHAC on the osteosarcoma MG63 cells, and its bone repair capacity, were investigated in vivo and in vitro. The PLGA copolymer was utilized as a drug carrier to deliver ADM–PLGA nanoparticles (ADM–PLGA–NP). Porous nano-hydroxyapatite and collagen were used to materials to produce the porous nano-hydroxyapatite/collagen scaffold (NHAC), into which the ADM–PLGA–NP was loaded. The performance of the drug-carrying scaffold was assessed using multiple techniques, including scanning electron microscopy and in vitro extended release. The antineoplastic activities of scaffold extracts on the human osteosarcoma MG63 cell line were evaluated in vitro using the cell counting kit-8 (CCK8) method and live-dead cell staining. The bone repair ability of the scaffold was assessed based on the establishment of a femoral condyle defect model in rabbits. ADM–PLGA–NHAC and NHAC were implanted into the rat muscle bag for immune response experiments. A tumor-bearing nude mice model was created, and the TUNEL and HE staining results were observed under optical microscopy to evaluate the antineoplastic activity and toxic side effects of the scaffold. The composite scaffold demonstrated extraordinary extended-release properties, and its extracts also exhibited significant inhibition of the growth of osteosarcoma MG63 cells. In the bone repair experiment, no significant difference was observed between ADM–PLGA–NHAC and NHAC by itself. In the immune response experiments, ADM–PLGA–NHAC exhibited remarkable biocompatibility. The in vivo antitumor experiment revealed that the implantation of ADM–PLGA–NHAC in the tumor resulted in a improved antineoplastic effect and fewer adverse side effects than direct intraperitoneal injection of ADM. The ADM–PLGA–NHAC developed in this study exhibited excellent extended-release drug properties, bone repairing and antineoplastic efficacy, which make it a promising osteoconductivity material with the capability to inhibit osteosarcoma.     

Methylene blue photocatalytic degradation under visible irradiation of Al doped ZnO powders by hydrothermal synthesis sensitized with octa-iso-pentyloxyphthalocyanine lead

AZO powders were sensitized through chemisorption method by octa-iso-pentyloxy phthalocyanine lead and characterized by Fourier transform infrared spectroscopy and X-ray photoelectron spectroscopy. The results showed that after sensitization process AZO photo physical properties improved greatly in visible regions. Photocatalytic degradation of methylene blue was studied under visible irradiation in aqueous solution and the pseudo first order model was used to obtain kinetic information of the photocatalytic degradation. The results indicated that photocatalytic activities of PbPc(iso-PeO)₈-AZO were better than of AZO powders.     

油气回收装置对加油机计量系统的影响

本文介绍了加油机的计量原理以及加油站油气回收系统的原理,对油气回收系统对加油机计量性能的影响做了定量分析实验,得出油气回收系统会影响到加油机示值误差的结论,并提出应对措施。     

基于Fluent的圆柱齿轮流量计的密封性仿真分析

通过对圆柱齿轮流量计进行二维简化建模,用Fluent仿真软件的ICEM CFD模块进行网格划分,利用Fluent仿真软件对圆柱齿轮流量计进行了计算和密封性的仿真分析,得出了其速度矢量分布图,并且给出了各泄漏量的计算公式和每种泄漏所占泄漏总量的百分比,即为以后的整个流量计的设计提供理论依据。,提高流量计的寿命具有重要意义。同时还省去了大量的人工计算过程,极大地加快了计算速度。     

光纤光栅形状传感器的研究

传感器总长810mm,直径为2.5mm,4根光纤布喇格光栅(fiber Bragg grating,FBG)互成90°分布在用记忆合金丝(Shap Memory Alloy,SMA)做基材的表面。通过在波分复用的基础上添加光时分复用来改进传感网络布置,提高测量精度;同时,设计了一套封装装置来确保封装时FBG与基材之间的准确定位以及黏结剂能够均匀的涂覆在基材和FBG表面,提高传感器的封装精度。实验结果表明,该FBG形状传感器的测量精度为3.1%。