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81.
82.
Avidin-containing polyelectrolyte microcapsules were prepared by a layer-by-layer deposition technique and uptake and release
of biotin-labelled fluorescein (b-FITC) was studied. The polyelectrolyte microcapsules were prepared by coating the surface
of calcium carbonate (CaCO3) microparticles containing avidin-poly(styrene sulfonate) (PSS) conjugate, followed by dissolution of CaCO3 core in an ethylenediaminetetraacetic acid solution. Release of avidin from the microcapsules was markedly suppressed due
to formation of a high molecular weight of avidin-PSS conjugate in the microcapsules. The uptake of b-FITC into the microcapsules
was highly enhanced through a strong binding of b-FITC to avidin, as compared to the uptake into avidin-free microcapsules.
Release of b-FITC from the microcapsules was accelerated upon addition of biotin, 2-iminobiotin, or lipoic acid in the solution
due to the competitive binding of the additives to the binding site of avidin. 相似文献
83.
Yoshihiro Itoh Fumiya Sahara Kaori Ozaki Ryo Akasaka Akira Teramoto 《Polymer Bulletin》2011,67(8):1455-1462
Emulsion polymerizations of several vinyl monomers, styrene, methyl methacrylate, butyl methacrylate, butyl acrylate, and
vinyl acetate, in water using alkali–hydrolysable cationic surfactants with a betaine ester group (1-alkoxycarbonylmethyl)trimethylammonium
chlorides, as emulsifiers were carried out and properties of the resulting latices and the polymers recovered by hydrolysis
and salting out were investigated. There were little influences of the surfactants and monomers used here on the polymerizations,
forming stable and monodisperse latices with a mean diameter of ca. 70 nm and giving a high molecular weight of polymers at
high yields. All polymers were precipitated and recovered by adding a small amount of sodium hydroxide into the latex solutions
contained little amount of ionic species. Solvent-cast films of the polymers were found to have surfaces as hydrophobic as
those for the corresponding pure polymers prepared by bulk polymerization. 相似文献
84.
Matsuda T Zhou D Okuno Y 《Journal of the Optical Society of America. A, Optics, image science, and vision》2002,19(4):695-701
We numerically investigate plasmon-resonance absorption of incident light energy by a bisinusoidal metal grating, i.e., one whose surface profile is sinusoidally corrugated in two orthogonal directions with a common period. Employing Yasuura's modal expansion method, we solve the problem of plane-wave diffraction by the grating and evaluate the absorption, which is observed as dips in diffraction efficiency curves. We examine the field distribution and energy flow in detail at the angles of incidence at which the absorption occurs. We show that the absorption is caused by coupling of the TM component of an evanescent order with surface plasmons. A phase-matching condition is used in the prediction of the incident angle at which the absorption occurs. This, together with the field profile in the presence of the resonance absorption, explains the mechanism of the absorption. We then illustrate interesting features of the absorption: enhancement of polarization conversion between the incident light and the reflected light and simultaneous excitation of two plasmon waves in directions that are symmetric with respect to the plane of incidence. 相似文献
85.
Kazunori Komatani Shinichi Ueno Tatsuya Kawahara Hiroshi G. Okuno 《User Modeling and User-Adapted Interaction》2005,15(1-2):169-183
We address the issue of appropriate user modeling to generate cooperative responses to users in spoken dialogue systems. Unlike
previous studies that have focused on a user’s knowledge, we propose more generalized modeling. We specifically set up three
dimensions for user models: the skill level in use of the system, the knowledge level about the target domain, and the degree of urgency. Moreover, the models are automatically derived by decision tree learning using actual dialogue data collected by the system.
We obtained reasonable accuracy in classification for all dimensions. Dialogue strategies based on user modeling were implemented
on the Kyoto City Bus Information System that was developed at our laboratory. Experimental evaluations revealed that the
cooperative responses adapted to each subject type served as good guides for novices without increasing the duration dialogue
lasted for skilled users. 相似文献
86.
Toshihiko Takaya Hidenori Kawamura Yoshihiro Minagawa Masahito Yamamoto Azuma Ohuchi 《Artificial Life and Robotics》2006,10(2):177-184
Blimp robots are attractive as indoor flying robots because they can float in the air, land safely with low energy, and stay
in motion for a long time compared with other flying robots. However, controlling blimp robots is difficult because they have
nonlinear characteristics, are influenced by air streams, and can easily be influenced by inertia. Therefore, a robust and
adaptive control system is needed for blimp robots. The applied research that has studied the features of indoor flying robots
in recent years has prospered. Operating an indoor blimp robot for a long time is difficult because the payload is small,
multiple batteries cannot be stacked, and the design of a thruster that gives freedom to the entire blimp robot is difficult.
Therefore, an autonomous charge that allows operation for a long time is needed. We have developed a method of landing with
orbital control of the charge point that gives autonomy to a blimp robot. The possibility of landing with orbital control
is shown.
This work was presented in part at the 10th International Symposium on Artificial Life and Robotics, Oita, Japan, February
4–6, 2005
An erratum to this article is available at . 相似文献
87.
The influence of the Fermi surface anisotropy on the specific heat jump is studied for the p-wave superconductor with a gap function d = z^(k
x
± ik
y
) in connection with Sr2RuO4. It is shown that the normalized specific heat jump, C/T
c
, is reduced from its universal value, 1.43, in accordance with experimental findings. We show that this behavior fits well into our present understanding of the quasiparticle spectrum and the impurity effects. 相似文献
88.
89.
Distribution and biliary and fecal excretion of bile acids were examined in Wistar strain male rats of about 300 g body weight.
The pool size of the rats on ordinary diet was 40 mg/rat, biliary secretion was 14 mg/hr, and fecal excretion was 10 mg/day.
Bile acids were mainly located in the small and large intestinal contents, 87% and 10%, respectively; but a portion was found
in the intestinal wall and the liver. Rats fed 2% cholesterol-supplemented diet for a week showed similar values for pool
size and biliary secretion with the rats on ordinary diet, but higher values for fecal excretion and distribution ratio in
the large intestinal contents. Cholic acid was a major component in the bile, small intestinal wall, small intestinal content
and liver, while the bile acid composition ratios were roughly similar to each other, although a relatively large amount of
α-muricholic acid was found in the intentinal wall and liver. Both the wall and content compositions of the large intestine
were similar to that of the feces, in which lithocholic, deoxycholic, α- and β-muricholic acids were the main components,
although the ratios of α- and β-muricholic acids in the large intestinal wall were larger than those in the intestinal contents
or feces. The high concentrations of these bile acids may indicate a difference of transport velocity across the cell membrane,
but the mechanism is not known. 相似文献
90.
An exact analysis of the dynamic problem on the thermal displacements and stresses of cylindrical shells subjected to instantaneous heating is carried out. Equations of motion for cylindrical shells, including the thermal effects, are reduced and solved by using the Fourier and Laplace transformation methods, after obtaining the temperature distribution determined from the nonstationary equation of heat conduction for a shell under a prescribed temperature field. Numerical results are presented for the thermal displacement and stresses due to the time variation together with the quasistatic ones. 相似文献