1.5 μm CMOS gate arrays with analog/digital macros designedusing common base arrays

Mixed analog and digital circuits are realized on a 1.5 μm silicon-gate CMOS chip with +5 V power supply only. The circuit uses CMOS digital gate arrays of 0.32 K to 19.6 K cells and is created without any additional turnaround time or any restriction on the design. Typical internal digital gate (two-input NAND) speed, with a fanout of 3 and a wire length of 3 mm, is 1.4 ns. A voltage comparator with ±8 mV maximum input offset voltage and 60 ns response time, digital-to-analog and analog-to-digital converters with 4-, 6-, and 8-bit resolution, respectively, and an analog switch of 25 Ω on-resistance can be realized on the same chip with digital circuits. Using this technology, about one-tenth of the turnaround time can be achieved compared with full-custom LSIs for the same system. The product development flow and computer-aided-design tools for designing mixed analog and digital gate arrays are the same as for digital gate arrays     

Acoustic emission and disturbances in central solenoid model coil for International Thermonuclear Experimental Reactor

This paper presents acoustic emission (AE) signals induced from the Central Solenoid (CS) model coil of the International Thermonuclear Experimental Reactor (ITER) program. Envelopes of the AE signals were quasi-continuously measured with high-time resolution of 100 μs using six multi-channel AE sensors in order to detect the disturbances in the CS model coil during the process of the series of direct current (DC) operations. The AE signals were considered to be originally induced by motion of superconducting cables in cable-in-conduit (CIC) conductors and local motion of the conductors, judging from the close correlation between the AE signals and voltage spikes in the coil, especially during the virgin current operation. The multi-channel measurements provide us with information about the distribution of disturbances that could be detected acoustically by the AE sensors installed at each point of the CS model coil. The observation of AE envelopes with high-time resolution showed that the disturbances at each location of the CS model coil decreased by repeatedly charging-up the coil, judging from instantaneous AE levels, AE energy and the number of AE events. Direct measurements of the number of AE events that were carried out at another point on the coil confirmed that the disturbance dependence on the number of operations was similar to that mentioned above. The transfer function methods using one pair of AE sensors enabled us to analyze changes that might occur in either the coil structure or in the disturbance in frequency region during the repeating of the charging-up processes of the coil.     

Pressure Effect on BDA-TTP Conductors

The pressure-induced conducting behavior of the charge-transfer salts (BDA-TTP)₂X [BDA-TTP = 2,5-bis(1,3-dithian-2-ylidene)-1,3,4,6-tetrathiapentalene; X = ClO₄·H₂O, ClO₄, BF₄·H₂O, BF₄, and I₃] has been investigated. With increasing pressure, the transition temperature to insulator observed in the ClO₄·H₂O, ClO₄, BF₄·H₂O, and BF₄ salts increases. On the other hand, the resistance of the I₃ salt as a function of temperature at 10.0 kbar shows a drop attributable to a superconducting transition with an onset at 4.5 K. The superconducting transition temperature (T_c) can reach 8.2 K by applying a pressure of 10.3 kbar, but gradually decreases with further increases in the pressure.     

Development of AFM tensile test technique for evaluating mechanical properties of sub-micron thick DLC films

This paper describes mechanical properties of submicron thick diamond-like carbon (DLC) films used for surface modification in MEMS devices. A new compact tensile tester operating under an atomic force microscope (AFM) is developed to measure Young's modulus, Poisson's ratio and fracture strength of single crystal silicon (SCS) and DLC coated SCS (DLC/SCS) specimens. DLC films with a thickness ranging from 0.11 /spl mu/m to 0.58 /spl mu/m are deposited on 19-/spl mu/m-thick SCS substrate by plasma-enhanced chemical vapor deposition using a hot cathode penning ionization gauge discharge. Young's moduli of the DLC films deposited at bias voltages of -100 V and -300 V are found to be constant at 102 GPa and 121 GPa, respectively, regardless of film thickness. Poisson's ratio of DLC film is also independent of film thickness, whereas fracture strength of DLC/SCS specimens is inversely proportional to thickness. Raman spectroscopy analyses are performed to examine the effect of hydrogen content in DLC films on elastic properties. Raman spectra reveal that a reduction in hydrogen content in the films leads to better elastic properties. Finally, the proposed evaluation techniques are shown to be applicable to sub-micron thick DLC films by finite element analyses.     

Osteoimmunology in Periodontitis: Local Proteins and Compounds to Alleviate Periodontitis

Periodontitis is one of the most common oral diseases resulting in gingival inflammation and tooth loss. Growing evidence indicates that it results from dysbiosis of the oral microbiome, which interferes with the host immune system, leading to bone destruction. Immune cells activate periodontal ligament cells to express the receptor activator of nuclear factor kappa-B (NF-κB) ligand (RANKL) and promote osteoclast activity. Osteocytes have active roles in periodontitis progression in the bone matrix. Local proteins are involved in bone regeneration through functional immunological plasticity. Here, we discuss the current knowledge of cellular and molecular mechanisms in periodontitis, the roles of local proteins, and promising synthetic compounds generating a periodontal regeneration effect. It is anticipated that this may lead to a better perception of periodontitis pathophysiology.     

Development of a fault locator for a branch line

Formerly, only pulse radar and the Murray loop bridge could be utilized in a cable fault location method that is applicable to branch lines. Those methods need a terminal connection at the far end. Therefore, it is difficult in fault location involving branch lines. Pulse radar is applicable only for high‐resistance ground faults and the Murray loop bridge is applicable only for low‐resistance ground faults. We have developed current‐detection pulse radar and tested it. In high‐resistance ground fault location, the measured result includes discharge delay error. Therefore, we have proposed an error correction method and confirmed it. In addition, we have designed a new cable fault location method of pulse radar and tested it. © 2000 Scripta Technica, Electr Eng Jpn, 134(2): 19–28, 2001     

Systematic mapping of autonomously replicating sequences on chromosome V of Saccharomyces cerevisiae using a novel strategy

We have developed a new procedure for easy and rapid identification of autonomously replicating sequences (ARSs) and have applied it to the analysis of chromosome V of Saccharomyces cerevisiae. The procedure makes use of the ordered λ phage clone bank of this chromosome that we have constructed, and includes transposition of a mini-transposon and selection of transposon-containing derivatives, isolation of their DNA and circularization at their cos-ends, transformation of yeast cells with the circularized DNA, and scoring transformation frequency. The transposon used was derived from Tn5supF, contained the yeast LEU2 gene, and was placed, together with the hyperactive transposase gene, on a mini-F plasmid for stable maintenance in Escherichia coli K-12. Sixteen regions of chromosome V showing ARS activity were identified, of which 12 were newly found in this work. Thus, the procedure will be useful for systematic genomic scale analysis of ARSs in yeast and related organisms in which ordered clone banks have been established. The average distance between adjacent ARS-containing regions was approximately 40 kb. Two-dimensional gel electrophoretic analysis of chromosome replication indicated that one of the newly identified ARSs was functional as an actual in situ replication origin, at least under the conditions employed.     

Dual pathways of tubular morphogenesis of vascular endothelial cells by human glioma cells: vascular endothelial growth factor/basic fibroblast growth factor and interleukin-8

In this study, we examined whether human glioma cells are angiogenic in a model using human microvascular endothelial cells, and also which factor is responsible for the glioma-dependent angiogenesis. Tubular morphogenesis in type I collagen gel by human microvascular endothelial cells was stimulated in the presence of 10 and 100 ng/ml of vascular endothelial growth factor (VEGF), 10 ng/ml basic fibroblast growth factor (bFGF) and 10 ng/ml of interleukin-8 (IL-8). Tube formation of the microvascular endothelial cells was assayed in the glioma cell lines IN157 and IN301, co-cultured using the double chamber method. IN301 cells had much higher levels of VEGF, bFGF and transforming growth factor-beta mRNA than IN157 cells, whereas the two had similar levels of transforming growth factor-alpha mRNA. By contrast, IN157 cells had much higher levels of IL-8 mRNA than IN301 cells. IN301-dependent tubular morphogenesis was inhibited by anti-VEGF or anti-bFGF antibody, and the inhibition was almost complete when anti-VEGF and anti-bFGF antibodies were present. On the other hand, IN157-dependent tubular morphogenesis was inhibited by anti-IL-8 antibody, but not by anti-VEGF or anti-bFGF antibodies. These findings demonstrated dual paracrine controls of tumor angiogenesis by human glioma cells. One is mediated through VEGF and/or bFGF, and the other, through IL-8.