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31.
G Bullock S Ray J Reed T Miyashita A Maria Ibrado Y Huang K Bhalla 《Canadian Metallurgical Quarterly》1995,1(5):559-564
Previous reports have demonstrated that a variety of anticancer drugs, e.g., 1-beta-D-arabinofuranosylcytosine (ara-C), mitoxantrone, etoposide, camptothecin, and cisplatin, induce the expression of c-jun oncogene in leukemic cells prior to producing internucleosomal DNA fragmentation and the morphological features of apoptosis. This has led to the impression that the induction of c-jun expression may be directly involved in the molecular signaling of the final common pathway of programmed cell death or apoptosis. In the present study, we examined the role of c-jun expression in three different settings of anticancer drug-induced apoptosis in human leukemic cells. First, exposure of human myeloid leukemia HL-60 cells to high-dose ara-C for 4 h produced internucleosomal DNA fragmentation preceded by c-jun induction. However, pretreatment of HL-60 cells with staurosporine, a protein kinase C inhibitor, repressed c-jun yet enhanced DNA fragmentation and apoptosis due to ara-C. Second, in human pre-B leukemia 697/BCL-2 cells which are transfected with the cDNA of the bcl-2 oncogene and overexpress p26BCL-2, although ara-C or mitoxantrone treatment caused greater c-jun induction than in the 697/neo cells, significantly reduced endonucleolytic DNA fragmentation and apoptosis was observed in 697/BCL-2 cells. Finally, taxol-induced internucleosomal DNA fragmentation and morphological features of apoptosis in HL-60 cells were not associated with the induction of c-jun expression. These lines of evidence indicate that the induction of c-jun expression may not have a direct role in the molecular signaling of anticancer drug-induced apoptosis, and that the anticancer drug-induced apoptosis can occur by a mechanism that does not involve the induction of c-jun expression. 相似文献
32.
33.
Low-loss single-mode fibres with GeO2-doped SiO2 cores have been made by the c.v.d. method and the loss spectrum from 0.6 to 1.8 ?m measured. The minimum loss of the fibre with suitably designed parameters, characterised by the refractive-index difference and the core diameter, was 0.75 dB/km at 1.05 ?m. 相似文献
34.
Katsumi Yoshino Tsuyoshi Demura Masaki Kawahigashi Yoshitsugu Miyashita Kiyoshi Kurahashi Yoshiji Matsuda 《Electrical Engineering in Japan》2004,146(1):18-26
Cross‐linked polyethylene (XLPE) has been widely adopted as insulating material for high‐voltage power cables up to 500 kV. Further improvement of electrical and thermal properties on insulating material is required in order to increase cable operation efficiency. Therefore, the development of novel insulating material possessing high thermal properties will be necessary. Recent progress of catalysis technology contributes to obtain new polymeric materials which may be applied to electrical insulation. The authors investigated the basic properties of newly developed stereoregular syndiotactic polypropylene (s‐PP) which is synthesized with homogeneous metallocene catalyst. Though recycling of cross‐linked polymers such as conventionally used XLPE may be difficult because of their poor heat deformation, the s‐PP which is not cross‐linked must be suitable for recycling. A series of experiments on its physical and electrical properties gave the following results.
- (1) s‐PP has sufficient flexibility compared with isotactic polypropylene (i‐PP ).
- (2) Both AC and lightning impulse breakdown strength of s‐PP in spite of no cross‐linking are superior to those of XLPE in the temperature range from 25 to 90 °C.
- (3) Degradation by copper of s‐PP is less than that of i‐PP.
- (4) s‐PP/VLDPE blend shows sufficient brittleness temperature for use.
35.
Efficient construction of cDNA microarrays utilizing normalized cDNA libraries of Arabidopsis thaliana 总被引:1,自引:0,他引:1
Ando K Yamakawa S Miyashita K Yoshida K Yokota A Shinmyo A Kohchi T 《Journal of Bioscience and Bioengineering》2004,97(1):85-88
We have demonstrated the utility of normalization for efficient cDNA microarray preparation using Arabidopsis as a model. Nonredundant cDNAs including 5722 species were efficiently collected from random 7914 expressed sequence tags (ESTs) in four normalized cDNA libraries. The prepared microarrays were successfully used to monitor gene expression. These methodologies should be applicable to the study of other species in plant biotechnology. 相似文献
36.
This article demonstrates the self-replication and self-organization phenomena based on a reaction–diffusion mechanism by
computer simulation. The simulation model consists of a one-dimensional cell array. Each cell contains two kinds of chemical
substances, activator u and inhibitor v, that can generate a reaction–diffusion wave, which is a spatial concentration pattern. The cells are supposed to be divided
or deleted depending on the concentrations of chemical substances. We tried several kinds of diffusion coefficient in the
model, and in some simulations, a self-replication process and a generating cell array with a metabolic process were observed.
By applying the division rule and the apoptosis rule, cell arrays duplicate in two oscillating states, i.e., self-replication
processes were observed. By applying a division rule and an annihilation rule, a cell array that has a stable length is generated
by changing the cell components, i.e., generating a cell array by a metabolic process was observed. Surprisingly, these two
phenomena are realized independently of the initial number of cells.
This work was presented in part at the 11th International Symposium on Artificial Life and Robotics, Oita, Japan, January
23–25, 2006 相似文献
37.
The frictional properties of an immobilized fluorinated polymer nanosheet were investigated to develop an ultrathin solid lubrication film with an excellent wear-resistant. N-(1H,1H-Pentadecafluorooctylmethacrylamide) copolymers containing carboxyl group as a reactive moiety form a stable monolayer on the water surface and a highly ordered reactive fluorinated polymer nanosheet can be fabricated by the Langmuir-Blodgett technique. The reactive fluorinated polymer nanosheet was immobilized onto solid substrates through chemical binding with an aminosilane coupling agent, and the film showed smooth surface even after the reaction. The immobilized fluorinated polymer nanosheet had a low friction coefficient and strong wear-resistant. 相似文献
38.
39.
Y Ninomiya T Kishimoto T Yamazawa H Ikeda Y Miyashita H Kasai 《Canadian Metallurgical Quarterly》1997,16(5):929-934
Age is the most important factor for the durability of biological valves. With an original design the Carpentier-Edwards pericardial valve showed improved results at 10 years. The influence of age on valve related complications is studied with a 10 year follow up on 807 valvular replacements. METHODS: Between January 1984 and December 1993, 807 patients underwent valve replacements with a Carpentier-Edwards pericardial bioprosthesis. Patients, 193 were younger than 60 years, 284 between 60 and 70 years and 330 patients were older than 70 years. All patients but seven were followed up for an average of 4.18 years after their operation and total follow up was 3373 patient years. Patients were divided into three groups of age: group I, less than 60 years; group II, 60-70 years; group III, over 70 years. A retrospective comparison was made between age groups. RESULTS: At 11 years, valve related complications included 97 patients with 27 valve related deaths. Rates of valve related death increase with age linearized rate were 0.3, 0.6 and 1.2%, respectively. No difference was observed for rates of all valve related morbidity: 2.6, 2.4 and 3.5%, respectively. Risk of thromboembolism increased with age, linearized rates were: 0.3, 0.7 and 1.3%. Risk of deterioration and reoperation decreased with age, rates of deterioration were 0.8, 0.1 and 0%. Other valve related events had the same incidence in all groups. No statistical difference was observed between group II and group III for deteriorations and reoperations. CONCLUSIONS: The performance of the Carpentier-Edwards pericardial valve is the same at 10 years in group II and III. This study supports the clinical use of this tissue valve in patients over 60 years. The results in group I are satisfactory, nevertheless, a more durable biological valve is needed for young patients. 相似文献
40.
Nobukata H. Takagi S. Hiraga K. Ohgishi T. Miyashita M. Kamimura K. Hiramatsu S. Sakai K. Ishida T. Arakawa H. Itoh M. Naiki I. Noda M. 《Solid-State Circuits, IEEE Journal of》2000,35(5):682-690
We report a fast-programming, compact sense and latch (SL) circuit to realize an eight-level NAND flash memory. Fast programming is achieved by supplying optimized voltage and pulsewidth to the bit lines, according to the programming data. As a result, all data programming is completed almost simultaneously, and 0.67-MB/s program throughput, which is 1.7 times faster than conventional program throughput, is achieved. The compact layout of the SL circuit is made possible by four 3-bit latches sharing one unit of the read/verify control circuit. Using these techniques, we fabricated a 144-Mb, eight-level NAND flash memory using a 0.35-μm CMOS process, resulting in a 104.2-mm2 die size and a 1.05-μm2 effective cell size 相似文献