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101.
102.
Isobe K Shoji K Nakanishi Y Yokoe M Wakao N 《Journal of Bioscience and Bioengineering》2003,95(3):257-263
Cholesterol oxidase (CHO) with high stability in detergents was found from an isolated strain, Y-134, belonging to the gamma-subclass of Proteobacteria. CHO production reached its maximum by incubation at 30 degrees C for 12 d. It was purified from cell-free extract prepared by mixing the cells with 0.4% Triton X-100. The absorption spectrum of the purified enzyme exhibited maxima at 274 and 410 nm, and a shoulder at 330 nm. The molecular mass was 115 kDa with two identical subunits of 58 kDa. The enzyme oxidized cholest-5-en-3beta-ol (cholesterol) and 5alpha-cholestan-3beta-ol (dihydrocholesterol) at a high reaction rate, and the K(m) value for cholesterol was 65 microM. The stability of the enzyme was higher than other CHOs in nonionic detergents with high values of hydrophilelipophile balance (HLB) such as Triton X-450 and sodium cholate. NH2-terminal sequence analysis showed a high similarity to CHO from Burkholderia cepacia, but not to CHOs from Streptomyces or Brevibacterium. 相似文献
103.
淮河流域1960~2011年干湿时空变化特征 总被引:1,自引:0,他引:1
在分析淮河流域26个气象站1960~2011年降水变化的基础上,计算了流域年尺度和季尺度降水距平百分率和标准化降水指数(SPI),并分析了淮河流域近52年干湿时空变化。结果表明,淮河流域年尺度降水距平与SPI较为一致,季尺度降水距平较SPI变化敏感;干旱多发生于春季,且2000年后发生频次减少;空间尺度上,流域中西部地区有变湿润趋势,东部沿海地区有变干旱趋势,变化趋势呈现近似纵向分带。 相似文献
104.
Effect of essential oil compounds and plant extracts on decay and termite resistance of wood 总被引:2,自引:0,他引:2
S. Nami Kartal Won-Joung Hwang Yuji Imamura Yasuo Sekine 《Holz als Roh- und Werkstoff》2006,64(6):455-461
Essential oils and their derivatives have a long history of safe usage as antimicrobial agents in food industry. In this study various essential oils and extracts from plants were screened for their ability to inhibit wood decay and termite attack in laboratory decay and termite resistance tests using treated wood specimens. In the laboratory decay resistance tests, wood specimens treated with essential oil compounds were subjected to brown-rot fungus, Tyromyces palustris, and white-rot fungus, Trametes versicolor for three months. The specimens were also subjected to termite attack by subterranean termites, Coptotermes formosanus for three weeks in laboratory conditions. The formulation with cinnamaldehyde (DF3) was found to be effective against both the brown-rot and white-rot fungi used in the study. However, the formulation with cinnamic acid (DF8) was able to protect wood specimens against only the white rot fungus and about 50% mass loss occurred in the specimens subjected to the brown-rot fungus. The mass losses in the wood specimens treated with cassia oil containing formulation (DF4) showed that cassia oil was effective against both fungi. The wood tar oil and dodecanal compounds also inhibited fungal decay in the specimens. The wood specimens treated with the formulations used in termite resistance tests were more resistant against the termites when compared to specimens treated with the formulations in decay resistance tests. Even after severe weathering process, treated wood specimens showed resistance against the subterranean termites. Results suggest that essential oils and plant extracts might be important to develop new wood preservatives that are less harmful to the environment and humans than recently available ones. 相似文献
105.
Yuji Hatano Keita IshiyamaHirofumi Homma Kuniaki Watanabe 《International Journal of Hydrogen Energy》2007
Niobium subcarbide (Nb2C) was chosen as a material for non-porous intermediate layer to improve the high temperature durability of Pd–Nb composite membranes for hydrogen separation. A layer of Nb2C was prepared between Nb substrate and thin Pd films (100 nm), and the stability of Pd coating at elevated temperatures (573–773 K) was examined by hydrogen absorption experiments. Hydrogen permeability through the Nb2C layer appeared to be sufficiently high, and no noticeable deterioration was observed in hydrogen absorption rate under as-prepared conditions. The degradation in coating effect of Pd at elevated temperatures was substantially mitigated by Nb2C layer. Such improved durability was ascribed to retardation of open porosity development by Nb2C caused as a consequence of impeded interdiffusion between Pd and Nb. 相似文献
106.
Myo Than Htay Yuji Tani Yoshio Hashimoto Kentaro Ito 《Journal of Materials Science: Materials in Electronics》2009,20(Z1):341-345
ZnO nanowires were grown by an ultrasonic spray pyrolysis technique. The aspect ratio and size of the wire were dependent
mainly on the pH value of a precursor solution and the growth temperature. By high-resolution transmission electron microscopic
analysis and photoluminescence measurements, it was confirmed that the nanowires are monocrystalline with good optical quality. 相似文献
107.
KyoungHou Kim Ryo Aida YoungAh Kang Yutaka Ohkoshi Yasuo Gotoh Masanobu Nagura Hiroshi Urakawa 《Polymer》2009,50(19):4429-4431
The structure development in the continuous laser-heated drawing process of poly(ethylene 2,6-naphthalene dicarboxylate) (PEN) fiber was analyzed by in-situ X-ray diffraction measurement. Because of the rapid and uniform laser heating, and the resultant steady-state nature of the necking-drawing, the structure development after the on-set of necking could be measured in the time resolution of several hundred microseconds. We found for the first time the temporal appearance of meridional (001′) diffraction at several milliseconds after the on-set of necking indicating that the mesophase structure similar to the one reported for poly(ethylene terephthalate) was also formed in the initial stage of fiber structure development of PEN. The d-spacing of the (001′) diffraction 1.230 ± 0.003 nm was shorter than the c-axis lengths of both α and β crystals. 相似文献
108.
Shinobu Hashimoto Kentaro Hattori Ayuka Nakahashi Yuji Iwamoto 《Materials Research Bulletin》2009,44(1):70-3888
Pure-phase LiAl5O8 was selected as an oxide ceramic red phosphor material without dopants (color centers) and was synthesized using a self-flux method. The LiAl5O8 was formed by heating a powder mixture consisting of γ-Al2O3:Li2SO4 = 1:2 (molar ratio) at over 1100 °C for 1 h. Photoluminescence (PL) properties for the synthesized LiAl5O8 were investigated. The maximum intensity of the excitation spectrum for the photoluminescent emission of LiAl5O8 synthesized was at 274 nm. The peak intensity of the emission spectrum was at a wavelength of 667 nm (red color). The intensity of the peak emission spectrum increased with the heating temperature, i.e., the maximum peak intensity of the red emission spectrum was detected for the LiAl5O8 synthesized by heating at 1500 °C for 1 h. 相似文献
109.
Honda Y Kitaoka M Sakka K Ohmiya K Hayashi K 《Journal of Bioscience and Bioengineering》2002,93(3):313-317
The kinetic parameters of Cex, a family 10 xylanase from Cellulomonas fimi, were determined at various pH levels using soluble birchwood xylan (BWX) as a natural polymeric substrate along with three other synthetic aryl-beta-D-xylobioside substrates. Using BWX, a high level of substrate inhibition was observed which increased with decreasing pH. In contrast, typical Michaelis-Menten-type profiles were obtained using the three aryl-beta-D-xylobiosides as substrates. The k(cat) values determined using o-nitrophenyl-beta-D-xylobioside did not change as the pH increased, whereas the k(cat) values obtained with BWX, phenyl-beta-D-xylobioside and p-nitrophenyl-beta-D-xylobioside decreased, suggesting that the presence of an ortho nitro group affects the activity displayed by Cex. These differences were not observed with XynB from Clostridium stercorarium F9, a member of the same family of xylanases as Cex. These results indicate that a careful evaluation is required when employing substituted aryl-beta-D-xylobiosides in the characterization of xylanases. 相似文献
110.
Masayuki Fujii Kazuyuki Tohyama Tetsuro Tokoro Masamitsu Kosaki Yuji Muramoto Naohiro Hozumi Masayuki Nagao 《Electrical Engineering in Japan》2002,141(2):8-16
Polymer materials have excellent dielectric and insulation properties; however, those properties in AC high field region have not been known well. Recently we established an evaluation method of high‐field AC dissipation current waveform of polymer materials 1 . AC dissipation current waveforms of polyethylene and polypropylene films show nonlinear distortion in AC high‐field region. This nonlinearity was thought to be related to the behavior of AC space charge formation in the sample near electrodes. The properties of space charge formed under AC high field at power frequency seem to differ from those formed under DC high field. The measurement of AC space charge distribution is not so easy due to the resolution limit of the space charge measurement. We studied the dielectric properties of biaxially oriented polypropylene (BOPP) film under AC high field up to 120 °C. It was found that tan δ, AC dissipation current (Ixr), and unbalanced component of capacitive current (ΔIxc) increased when the temperature became higher. In particular, ΔIxc increased above some threshold field and was considered to be due to the AC space charge formation. This AC space charge layer near electrode is thought to be formed due to carrier injection under AC high‐field application. Usually, the carrier mobility becomes smaller on lowering the temperature. Most of the carriers injected from the electrode are trapped near the electrode in the sample film. But in the high‐temperature region, the carrier mobility becomes larger and the carrier injection starts to increase from lower field. Many more carriers are injected from the electrode. It is thought that some of the injected carriers are trapped inside the sample film; the others go through the sample to the opposite side. © 2002 Wiley Periodicals, Inc. Electr Eng Jpn, 141(2): 8–16, 2002; Published online in Wiley InterScience ( www.interscience.wiley.com ). DOI 10.1002/eej.10018 相似文献