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71.
D Miyamoto N Endo N Oku Y Arima T Suzuki Y Suzuki 《Canadian Metallurgical Quarterly》1998,21(12):1258-1262
The cytotoxic effects of beta-thujaplicin and five kinds of metal chelates were examined on mouse melanoma B16BL6 cells by cell viability and lactate dehydrogenase (LDH) release assay. Beta-thujaplicin-zinc chelate and beta-thujaplicin-copper chelate had higher cytotoxic effects than beta-thujaplicin, and the 50% effective doses (ED50) of these metal chelates were 12.5 and 25 microM, respectively. In addition, the zinc chelate induced DNA ladder formation in B16BL6 cells, as shown by the DNA fragmentation assay, suggesting that cell death induced by the zinc chelate is apoptosis. The zinc chelate also had a cytotoxic effect and induced DNA fragmentation on other tumor cell lines: HeLa, Meth A, and B16F1 cells, but not on normal human diploid fibroblasts FS-4. These results suggest that beta-thujaplicin-zinc chelate induces apoptotic cell death in various tumor cell lines and is a potent antitumor agent for tumor cells including malignant melanomas. 相似文献
72.
When a fault occurs on transmission or distribution systems due to lightning or overvoltage, often an arc discharge occurs at the fault point. The arc discharge, which is caused by a fault current, has a high current, high temperature, strong light emission, etc., thus it sometimes causes heavy damages to electric power equipment. The arc discharge is influenced by the conditions around the arcs, i.e., gas, insulation materials, gap length, weather, etc. Also, the arc voltage along the arc column indicates the characteristics of the arc. If the voltage waveforms of the arcs caused by the fault on transmission or distribution systems are classified, it is possible to find the location and the equipment where the fault occurred. In this paper, the arc voltage data in 6-kV class XLPE cables and 6-kV class overhead lines are analyzed and an artificial neural network method is applied to classify the arc voltage waveforms. The results obtained from the six artificial neural networks developed show that the artificial neural network method is effective for classification of arc voltage waveforms if adequate input parameters are selected. 相似文献
73.
RJ Rottier CN Hahn LW Mann M del Pilar Martin RJ Smeyne K Suzuki A d'Azzo 《Canadian Metallurgical Quarterly》1998,7(11):1787-1794
Protective protein/cathepsin A (PPCA) is a pleiotropic lysosomal enzyme that complexes with beta-galactosidase and neuraminidase, and possesses serine carboxypeptidase activity. Its deficiency in man results in the neurodegenerative lysosomal storage disorder galactosialidosis (GS). The mouse model of this disease resembles the human early onset phenotype and results in severe nephropathy and ataxia. To understand better the pathophysiology of the disease, we compared the occurrence of lysosomal PPCA mRNA and protein in normal adult mouse tissues with the incidence of lysosomal storage in PPCA(-/-) mice. PPCA expression was markedly variable among different tissues. Most sites that produced both mRNA and protein at high levels in normal mice showed extensive and overt storage in the knockout mice. However, this correlation was not consistent as some cells that normally expressed high levels of PPCA were unaffected in their storage capability in the PPCA(-/-) mice. In addition, some normally low expressing cells accumulated large amounts of undegraded products in the GS mouse. This apparent discrepancy may reflect a requirement for the catalytic rather than the protective function of PPCA and/or the presence of cell-specific substrates in certain cell types. A detailed map showing the cellular distribution of PPCA in nomal mouse tissues as well as the sites of lysosomal storage in deficient mice is critical for accurate assessment of the effects of therapeutic interventions. 相似文献
74.
75.
Surface characteristics of the bovine oocyte and its investments before, during, and after maturation, and fertilization in vitro were evaluated by scanning electron microscopy (SEM). Oocyte diameters were also measured during SEM analysis of the oocyte. The cumulus cells manifested a compact structure with minimal intercellular spaces among them in the immature oocytes. These became fully expanded with increased intercellular spaces after maturation in vitro, but contracted again after fertilization. The zona pellucida (ZP) showed a fibrous, open mesh-like structure in the maturing and matured oocytes. The size and number of meshes on the ZP decreased dramatically after fertilization. The vitelline surface of immature oocytes was characterized by distribution of tongue-shaped protrusions (TSPs) varying in density. After 10 and 22 hr of maturation incubation, oocyte surface microvilli (MV) increased to become the predominant surface structure, and TSPs decreased substantially. The vitelline surface of fertilized oocytes (at 6 and 20 hr) was similar to that of the matured oocytes, but unfertilized oocytes had less dense MV than did fertilized oocytes (at 20 hr). The diameter of the oocytes decreased from 99 to 80 microns during maturation and increased to 106 microns after insemination (P < 0.05). Membrane maturation was characterized by surface changes from a TSP-predominant pattern to a MV-predominant pattern. Thus, the bovine oocyte maturation process was found to involve the expansion of cumulus cells and the maturation of the ZP, which changes dramatically upon fertilization. Also, volumetric changes occurred in ooplasm processed for SEM following oocyte maturation and insemination. 相似文献
76.
The preparation and characterization of indium oxide (InO
x
)/tin oxide (SnO
y
) multilayered films deposited by ion-beam sputtering are described and compared with indium tin oxide (ITO) films. The structure and the optoelectrical properties of the films are studied in relation to the layered structures and the post-deposition annealing. Low-angle X-ray diffraction analysis showed that most films retained the regular layered structures even after annealing at 500° C for 16 h. As an example, we obtained a resistivity of 6×10–4 cm and a transparency of about 85% in the visible range at a thickness of 110 nm in a multilayered film of InO
x
(2.0 nm)/SnO
y
(0.2 nm)×50 pairs when annealed at 300° C for 0.5 h in air. Hall coefficient measurements showed that this film had a mobility of 17 cm2 V–1 sec–1 and a carrier concentration (electron density) of 5×1020 cm–3. 相似文献
77.
M. Jiménez-Melendo J. P. Riviére T. Suzuki H. Koizumi J. Castaing A. Domínguez-Rodríguez 《Journal of Materials Science》1992,27(13):3589-3593
NiO single crystals prepared by two crystal growth techniques (zone melting in an arc-image furnace and Verneuil crystallization have been deformed by compression along 001 at temperatures as low as 4.2 K, and the dislocation substructure observed by transmission electron microscopy. The Peierls mechanism has been suggested as the mechanism controlling the mechanical behaviour at the lower temperatures. The dislocations generated at cavities found in the zone-melted crystals may be responsible for the increase of the flow stress of these crystals compared with the Verneuil ones. 相似文献
78.
The surface tension of liquid3He and4He was measured near the gas-liquid critical points in the reduced temperature range 3×10–4–1, where t (T
c
–T)/T
c
. The critical exponents were found to be 3=1.289±0.015 for3He and 4=1.306±0.017 for4He. These values are very close to those for classical liquids, and are consistent with the value of 1.28 predicted by Widom, but are apparently different from the exponents previously obtained for liquid helium isotopes, which are near unity. The critical coefficients show good agreement with the quantum-corrected corresponding states theory for the Lennard-Jones 6–12 potential discussed by Young. The interface thickness is deduced from Widom's theory to bed=d
0t–v withd
30=0.14±0.03nm and v3=0.57±0.04 for3He, andd
40=0.37±0.07 nm and v4=0.58±0.01 for4He. 相似文献
79.
The behaviour of the amorphous chain in vulcanized natural rubber (NR) and styrene-butadiene rubber (SBR) under extension is studied by means of the cylindrical distribution function (CDF) derived from wide angle X-ray scattering (WAXS). In the case of the highly stretched vulcanized NR where the orientation-induced crystallization took place, the observed WAXS intensity was separated into a crystalline peak component and a broad amorphous one from which the CDF was obtained. The CDF calculated from the broad component shows that some of the amorphous segments of the highly stretched vulcanized NR are extended as fully as the crystalline chains and that the fraction of the segments increases with increasing extension. In the case of SBR, such fully stretched segments as in the case of the vulcanized NR show higher degree of an interchain packing order than those in the vulcanized NR. 相似文献
80.