基于电力大数据的配电网健康管理方法

配电网的安全稳定运行,对提升供电可靠性、保障用户电能质量、提高电网企业综合效益具有十分重要的意义.以杭州市萧山区为例,介绍基于电力大数据的配电网健康管理方法.     

特高压直流接地极线路电动越障双线飞车设计与研制

针对现有的导线飞车不适用于特高压直流接地极线路环境,跨越间隔棒、防振锤等障碍不便的问题,设计了一款特高压直流接地极线路电动越障双线飞车.该装置体积小、质量轻、使用简单,可适用于特高压直流接地极或其他双分裂导线带电或停电走线作业,具备自动越障功能,稳定可靠,具有较高的经济效益和实用价值.     

配电线路不停电作业机器人研究

从传统的人工带电作业方式出发,根据国内外不停电作业机器人的发展现状,对机器人带电作业方式进行分类,并结合具体实例,描述了我国不停电作业机器人的特点,对该领域存在的问题进行分析和展望.     

汽车涂装集中输调漆系统的发展现状

概述了汽车涂装集中输调漆系统的发展现状,描述了集中输调漆系统的一般构成,重点介绍了电动泵智能油漆循环系统和走珠式快速换色系统.     

盐酸酸洗废液制备α-纳米Fe2O3的工艺优化

以氧化铁皮作为除酸剂,与盐酸酸洗废液反应制得FeCl₂溶液,再采用空气-双氧水双重氧化和沉淀法从中制得α-Fe₂O₃纳米颗粒。分析了FeCl₂质量浓度、氨水质量浓度、超声时间和煅烧温度对产物的的粒径和纯度的影响。采用场发射扫描电子显微镜(SEM)和X射线衍射仪(XRD)对产物的形貌和结构进行了表征。优化实验结果表明,FeCl₂质量浓度为16 g/L,氨水质量浓度为7.5 g/L,超声时间为50 min,煅烧温度为750℃时,制得了分散性虽然一般,但纯度较高的α-Fe₂O₃,其含量高达95.3%,平均晶粒尺寸约为38.2 nm。这为盐酸酸洗废液的资源化利用提供了实验基础。     

大型复杂结构件抛丸改进方案

为解决某大型焊接结构件抛丸时工件上面大量堆积钢丸并可能导致安全事故的问题,从改变工件吊挂状态和在工件上增加漏丸工艺孔两方面入手,令工件可安全地在已有抛丸线上进行自动除锈.     

基于LSTM的电力暂态稳定在线评估及预测研究

电网规模的扩大使得电力系统运行状态变得更加复杂,对电网安全稳定运行提出了更高要求。提出了基于深度学习中长短时记忆（long-and-short term memory,LSTM）的电力暂态稳定在线评估模型。该模型通过获取全网各节点电压、电流、功率等电气量,实时计算得到电网失稳可能性评分,并在新英格兰10机39线系统上对该模型进行测试与优化。实验结果表明,该模型能通过实时运算得到电网稳定性的评估及预警,具有准确性高、预警能力强、支持在线监测的特点。     

IGBT串联均压方法综述

绝缘栅双极性晶体管IGBT(insulated gate bipolar transistor)在高电压场合应用时需串联使用满足电压需求。由于器件内部的性能差异和外围电路参数不一致等,引起IGBT模块之间电压不均衡问题,威胁其运行安全。综述了国内外IGBT串联均压方法的发展及其研究现状。根据均压方法机理的不同,将IGBT串联均压方法分为被动均压方法和主动均压方法两种,进一步将主动均压方法归纳为无源控制方法和有源控制方法两类。根据各类方法的基本电路拓扑分析了均压原理,梳理了不同方法在电路拓扑、参数选择和控制策略等方面的优化和最新进展。通过均压效果、附加损耗和可靠性等多方面对不同均压方法进行对比,被动均压方法拓扑简单不需外加控制电路更适合在低频应用场合,在高频应用场合中,准有源栅极控制法以单驱动与无源器件相结合的方式,具有良好的发展前景。最后对IGBT串联均压方法进行了展望。     

Soluble Prokaryotic Overexpression and Purification of Human GM-CSF Using the Protein Disulfide Isomerase b′a′ Domain

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a member of the colony-stimulating factor (CSF) family, which functions to enhance the proliferation and differentiation of hematopoietic stem cells and other hematopoietic lineages such as neutrophils, dendritic cells, or macrophages. These proteins have thus generated considerable interest in clinical therapy research. A current obstacle to the prokaryotic production of human GM-CSF (hGM-CSF) is its low solubility when overexpressed and subsequent complex refolding processes. In our present study, the solubility of hGM-CSF was examined when combined with three N-terminal fusion tags in five E. coli strains at three different expression temperatures. In the five E. coli strains BL21 (DE3), ClearColi BL21 (DE3), LOBSTR, SHuffle T7 and Origami2 (DE3), the hexahistidine-tagged hGM-CSF showed the best expression but was insoluble in all cases at each examined temperature. Tagging with the maltose-binding protein (MBP) and the b′a′ domain of protein disulfide isomerase (PDIb′a′) greatly improved the soluble overexpression of hGM-CSF at 30 °C and 18 °C. The solubility was not improved using the Origami2 (DE3) and SHuffle T7 strains that have been engineered for disulfide bond formation. Two conventional chromatographic steps were used to purify hGM-CSF from the overexpressed PDIb′a′-hGM-CSF produced in ClearColi BL21 (DE3). In the experiment, 0.65 mg of hGM-CSF was isolated from a 0.5 L flask culture of these E. coli and showed a 98% purity by SDS-PAGE analysis and silver staining. The bioactivity of this purified hGM-CSF was measured at an EC₅₀ of 16.4 ± 2 pM by a CCK8 assay in TF-1 human erythroleukemia cells.     

An Engineered Biliverdin-Compatible Cyanobacteriochrome Enables a Unique Ultrafast Reversible Photoswitching Pathway

Cyanobacteriochromes (CBCRs) are promising optogenetic tools for their diverse absorption properties with a single compact cofactor-binding domain. We previously uncovered the ultrafast reversible photoswitching dynamics of a red/green photoreceptor AnPixJg2, which binds phycocyanobilin (PCB) that is unavailable in mammalian cells. Biliverdin (BV) is a mammalian cofactor with a similar structure to PCB but exhibits redder absorption. To improve the AnPixJg2 feasibility in mammalian applications, AnPixJg2_BV4 with only four mutations has been engineered to incorporate BV. Herein, we implemented femtosecond transient absorption (fs-TA) and ground state femtosecond stimulated Raman spectroscopy (GS-FSRS) to uncover transient electronic dynamics on molecular time scales and key structural motions responsible for the photoconversion of AnPixJg2_BV4 with PCB (Bpcb) and BV (Bbv) cofactors in comparison with the parent AnPixJg2 (Apcb). Bpcb adopts the same photoconversion scheme as Apcb, while BV4 mutations create a less bulky environment around the cofactor D ring that promotes a faster twist. The engineered Bbv employs a reversible clockwise/counterclockwise photoswitching that requires a two-step twist on ~5 and 35 picosecond (ps) time scales. The primary forward P_fr → P_o transition displays equal amplitude weights between the two processes before reaching a conical intersection. In contrast, the primary reverse P_o → P_fr transition shows a 2:1 weight ratio of the ~35 ps over 5 ps component, implying notable changes to the D-ring-twisting pathway. Moreover, we performed pre-resonance GS-FSRS and quantum calculations to identify the Bbv vibrational marker bands at ~659,797, and 1225 cm⁻¹. These modes reveal a stronger H-bonding network around the BV cofactor A ring with BV4 mutations, corroborating the D-ring-dominant reversible photoswitching pathway in the excited state. Implementation of BV4 mutations in other PCB-binding GAF domains like AnPixJg4, AM1_1870g3, and NpF2164g5 could promote similar efficient reversible photoswitching for more directional bioimaging and optogenetic applications, and inspire other bioengineering advances.