PURIFICATION AND PROPERTIES OF TRANSGLUTAMINASE FROM STREPTOVERTICILLIUM MOBARAENSE

Transglutaminase (TGase) was separated from the culture broth of an isolated strain of Streptoverticillium mobaraense. The crude enzyme was prepared by centrifugation, ultrafiltration, precipitation by alcohol, centrifugation and freeze‐drying. The yield after these processes was 65–70%. Then the enzyme was purified to homogeneity by chromatography on CM‐cellulose and Sephadex G‐75 on which the yields were about 70% and 80%, respectively; the purified folds reached 2.5–4.7 and 1.08–2.06, respectively. The molecular weight of this TGase was 39,500–40,100 Da by gel filtration chromatography. Optimum enzyme activity was observed in the pH range of 5.0–7.0, and it was maintained stable at 20–40C. The optimal temperature and pH was 52C and 6.0, respectively. At 1 mM and 5 mM metal ion or inhibitors concentration, TGase activity was strongly inhibited by Zn²⁺ and NEM, and not affected obviously by Ba²⁺, Ca²⁺, Co²⁺, Cu²⁺, Fe²⁺, Fe³⁺, Mg²⁺, Mn²⁺, Na⁺ as well as PMSF and EDTA. The effects of these additions on this TGase were compared with those of other microbial TGases.     

粉煤灰降失水水泥浆在天然气井固井中的研究与应用

长庆天然气田属于低压、低渗透气田,存在承压能力较低的地层,固井设计要求全井段封固,并且采用双级双凝施工工艺。但由于用于低密度水泥浆中的漂珠生产量降低,价格上升,研究应用替代产品是固井技术发展的方向。在一级领浆中研究应用了粉煤灰水泥浆体系,其性能和固井质量可完全满足固井要求。粉煤灰水泥浆体系价格低和固井质量优良,使其成为漂珠水泥浆体系的替代配方。     

One-to-Many and Many-to-One Optical Chaos Communications Using Semiconductor Lasers

A theoretical construction of one-to-many (OTM) and many-to-one (MTO) chaos synchronization communications using semiconductor lasers is presented. One center laser provides strong injections (strong link) to other side lasers, and the side lasers also provide weak injections (weak link) to the center one. Simulation results show that the side lasers synchronize with the center laser through injection-locking. In addition, messages transmitted via the strong links or the weak links experience strong chaos pass filtering, enabling us to realize OTM and MTO communications.     

一种前馈型全摆幅恒跨导准恒增益CMOS运放设计

设计了一种适合在低电源电压下工作的前馈型输入级放大结构，在全摆幅的动态工作范围内，输入级跨导保持不变，采用负载电流补偿以保证增益近似恒定，输出采用前馈型AB类输结构，实现全摆幅输出。     

基于Delphi的报童模型的实现与动态仿真

已往库存论中的决策模型,它是把现实过程用数学的方法进行分析,得到决策值,但不能看到决策的过程,不能反映出现实生活中的不确定性、动态性。运用Delphi 7.0和SQL server 2000对销售过程进行了动态仿真,利用Newsboy模型的推广(S,s)订购策略来监控库存并做出决策,这样就体现出此过程的不确定性、动态性;且举出了一个仿真商品销售的实例,对其中的一些关键技术,如何仿真、算法的实现等进行了阐述。     

实用户外视频监控异常检测策略

树枝叶等植物的晃动是造成户外监控背景复杂性的主要因素。该文提出一种基于视频分析的运动对象检测策略，利用快慢双背射模型检测运动变化像素，并通过邻域像素的棚关性检测消除虚假运动对象。实验表明此算法对户外监控不但速度快而且检测效果理想，满足实用化要求。     

Determination and interpretation of fractal properties of the sandstone pore system

A knowledge of fractal properties of the pore system is extremely useful with regard to the interrelationships between moisture transport and pore structure. The fractal dimension characterises the egree of resolutiondependence of a property. Given a completely interlaced pore system such as that of sandstone, the fractal dimension of the pore volume, or the porosity, quantifies the cross-sectional variability of the pore channels and is thus of great relevance with regard to transport processes. The fractal dimension of the pore surface describes the surface roughness. A newly-developed measuing technique is presented for the simultaneous determination of fractal dimensions of porosity and pore surface and for establishing fraction radii distributions in order to derive relevant parameters for moisture transport.     

Determination of the width of the transition layer between the crystalline and amorphous zones in nylon 1010 by SAXS

The width of the transition layer between the crystalline and amorphous zones in nylon 1010 was determined by SAXS with point collimation and long-slit collimation, respectively. The width of the transition layer, E, was found to be 1.7 nm. The results show that the width of the transition layer is independent of crystallinity.     

Sirius叠前深度偏移在莺歌海盆地“泥拱模糊带”处理中的应用

在对Sirius叠前深度偏移软件应用开发的基础上,分析讨论了Sirius 2D叠前深度偏移速度模型建立、层析成像及演、叠前深度偏移以及深度聚焦等技术环节的基本原理,并以莺歌海盆地某测线的处理为例,具体分析了Sirius 2D叠前深度偏移处理的流程、主要参数及时效.文中还分析讨论了莺歌海盆地中央“泥拱带”叠前深度偏移处理的地质效果.实践表明,莺歌海盆地广泛发育的与“泥拱模糊带”有关的构造各有其特殊性,只有通过3D叠前深度偏移处理才能从根本上解决“泥拱模糊带”的成像问题.