TRPO－磺化煤油萃取乳酸的影响因素

报导了萃取发酵Ｌ－乳酸中的萃取和反萃取行为的研究。结果表明，在萃取过程中，无机盐（ＭｇＳＯ４、ＺｎＳＯ４、ＫＨ２ＰＯ４）、乳酸钙、葡萄糖和溶液初始乳酸的浓度均对分配比ＤＬａ有明显的影响；并对９０℃热水的反萃取进行了研究。反分配比Ｄ′Ｌａ随有机相初始酸浓的增加而逐渐下降；还对萃取和反萃取机理作了初步的探索     

Determination of Teicoplanin A2's Partition Coefficient in Different Liquid-Liquid Extraction Systems

Teicoplanin is one of the macrocyclic glycopeptide antibiotics, which is active against Gram-positive bacteria, and has attracted a lot of attention in the field of chiral separation recently. In this work, the partition coefficients and extraction ratio of teicoplanin in three different solvent systems were studied: conventional extraction, reactive extraction and reverse micelle extraction. With conventional solvent extraction, n-butanol demonstrated high partition coefficient for teicoplanin, but low extraction ratio because of its high solubility in water. Reactive extraction of teicoplanin showed the highest partition coefficient with almost 100% recovery in organic phase when tri-n-octylmethyl ammonium chloride (TOMAC) was used as extractant and pH value was above 5.0. A reverse micelle system, consisted of isooctane 10mmol-L-1 TOMAC 1% n-octanol, also offered high separation factor for teicoplanin. The results are beneficial for the design of teicoplanin separation and purification process.     

固定化米根霉在三相流化床中间歇发酵生产L－乳酸的研究

研究了海藻酸钙包埋法固定米根霉在三相流化床反应器中间歇发酵生产Ｌ－乳酸的工艺。实验结果表明，固定化颗粒具有较好的重复使用效果；并考察了颗粒装填密度、通气量、糖浓度及温度对产酸速率的影响，在通气量大于１．０ｖｖｍ、温度为３９℃、糖浓度为（１０～８０）ｇ／Ｌ的条件下，产酸速率达到２０．６ｇ／Ｌ·ｈ，糖对Ｌ－乳酸的转化率为（７０～８０）％；还建立了糖浓度和温度与产酸速率间的数学模型     

葡萄糖及磷酸盐对刺糖多孢菌多杀菌素发酵的影响

The effects of glucose and inorganic phosphate on mycelium growth and spinosad production with Saccharopolyspora spinosa were studied. The results showed that the increase of glucose concentration from 18.6g·L-1 to 58.8g·L-1 could promote the mycelium growth and spinosad production. And when the glucose concentration increased from 58.8g·L-1 to 79.6g·L-1, no obvious change was detected but a slight drop in spinosad production was observed, whereas, when the glucose concentration increased from 79.6g·L-1 to l15.3g·L-1, substantial decrease in both mycelium growth and spinosad production occurred. The increase of phosphate conccntration from 3.68mmol· L-1 to 29.41mmol·L-1 rendered corresponding increase in mycelium growth and spinosad production. When phosphate concentration increased from 29.41mmol· L-1 to 44.12mmol·L-1, mycelium growth slightly increased and spinosad production dropped, while when phosphate concentration increased from44.12mmol·L-1 to 57.62mmol·L-1, both mycelium growth and spinosad production decreased sharply. Conclusively,the optimal initial concentration of glucose and phosphate is 58.8g·L-1 and 29.41mmol·L-1, respectively. The spinosad fermentation in the production medium containing 58.8g·L-1 glucose and 29.41mmol·L-1 phosphate was scaled up in 5-L fermentation and the spinosad production reached 507mg·L-1, which was 28% higher than that in the flask fermentation.     

木兰假丝酵母不对称还原制备(S)-CHBE

利用木兰假丝酵母催化的4-氯乙酰乙酸乙酯(COBE)立体选择性还原反应，制备了具有光学活性的手性化合物 (S)-4-氯-3-羟基丁酸乙酯((S)-CHBE).分别考察培养条件、共底物种类及浓度、转化时间、转化温度、底物添加量、细 胞浓度和pH值等因素对产物得率和对映体过量值（e.e.）的影响.结果表明，在30 ℃、pH 6.0的磷酸钾缓冲液(100 mmol/L)中添加10 g/L共底物葡萄糖振荡转化时，9 g/L好氧培养的木兰假丝酵母能将12.1 g/L的4-氯乙酰乙酸乙酯几乎 全部地转化为(S)-4-氯-3-羟基丁酸乙酯，且e.e.值达到85.2％.     

CO和N_2气体混合物在Cu(Ⅰ)—13Ⅹ分子筛吸附柱上穿透曲线的研究

测量了CO和N_2气体混合物在不同组成和流速下在Cu(I)—13X分子筛吸附柱上的穿透曲线。建立了非等速、非等温、非绝热、非线性等温线的吸附柱动态行为数学模型,并从实验数据回归了吸附速率常数,计算的穿透曲线与温度变化曲线与实验数据符合良好。     

改进的Van der Waals方程推算双组分的吸附平衡

借鉴热力学状态方程在气液平衡中的经验,修改了二维van der Waals状态方程,并在气固吸附平衡中应用。从纯组分的吸附等温线出发,推算气和二氧化碳混和物及二氧化碳和一氧化碳的混和物分别在C_u(I)Y沸石上的吸附平衡。还用另外三个吸附模型的计算和比较。它们是理想吸附溶液模型,Flory—Huggins活度系数方程的吸附空穴溶液模型及二维van der Waals状态方程。结果表明,本文改进的方程推算实验数据的效果比其它模型好,尤其是关于一氧化碳和二氧化碳在C_u(I)Y石上的吸附,络合吸附和物理吸附是同时发生的。     

用拟化学溶液模型从液体混合热数据推算溶液的粘度

利用Rother等的关系式结合我们提出的拟化学溶液模型和由液体混合热数据拟合得到的模型参数,推算了苯—甲醇等五个体系的溶液粘度,计算值和文献值符合良好。     

S-腺苷-L-蛋氨酸在弱酸树脂上的离子交换平衡

结合Donnan膜平衡理论与树脂的弱电解质性质,在假设S-腺苷-L-蛋氨酸(SAM)分子带一价正电荷的基础上,建立了SAM在D113弱酸树脂上的离子交换平衡模型.实验测定了初始pH值为2.30、5.01、7.13及8.1 3时的等温离子交换平衡数据,通过实验数据拟合得到了两个模型参数.实验数据拟合结果表明,在初始pH值为5～8时,两参数的模型较好地描述了离子交换平衡.