Effect of mild acid treatment on the survival, enteropathogenicity, and protein production in vibrio parahaemolyticus

Vibrio parahaemolyticus is an important food-borne enteropathogen that encounters various adverse conditions in its native environment or during infection. Effects of mild acid treatment on survival under stress conditions, enteropathogenicity, and protein production in this pathogen were investigated. Logarithmically grown cells, at pH 7.5 shifted to pH 5.0 for 30 min, were more resistant to subsequent acid challenge at pH 4.4. A two-phase adaptive procedure (pH 5.8 for 30 min; pH 5.0 for 30 min) was better than a single-phase procedure for enhancing the acid tolerance of this pathogen. The acid-adapted cells were cross-protected against the challenges of low salinity and thermal inactivation. One-dimensional polyacrylamide gel electrophoresis revealed that proteins with molecular masses of 6.4, 9.0, 13.6, 16.3, 18.9, 22.9, 24.4, 28.3, 33. 9, 36.9, 41.2, 47.6, 58.1, 65.6, 80.5, 88.2, and 96.9 kDa were induced or significantly enhanced, while proteins of 25.3, 30.1, 30. 7, and 91.7 kDa were significantly inhibited. Two-dimensional polyacrylamide gel electrophoresis revealed that 20 species of proteins were induced or significantly enhanced, while 26 species were inhibited. In assays conducted using the suckling mouse model, enteropathogenicity of the acid-adapted cells was significantly enhanced in terms of intestine/body weight ratio and in vivo recovery of infected cells.     

TiAl基合金粉末爆炸烧结特性常数的测算

本文测算了自蔓延高温合成TiAl基合金粉末的爆炸烧结特性常数值：γ＝1．44，lgβ＝25．25，并为试验证实，这为获取大尺寸TiAl基合金提供了理论依据。     

Factors regulating SCC antigen expression in squamous cell carcinoma of the uterine cervix

Expression of squamous cell carcinoma (SCC) antigen emerged concurrently with squamous formation of the uterine cervix and increased during the neoplastic transformation of the cervical squamous epithelium. SCC antigen expression differed considerably among the histomorphologic cell types of cervical carcinoma. Large cell nonkeratinizing carcinoma contained high levels of the antigen. In contrast, no appreciable expression of SCC antigen was observed in small cell nonkeratinizing carcinoma. The pattern of SCC antigen expression closely coincided with EGF receptor (EGF-R) expression in cervical squamous neoplasia. This suggests that the expression of SCC and EGF-R in cervical carcinoma is related to the differentiation or dedifferentiation processes of the tumor cells. SCC production by CaSki cervical epidermoid carcinoma cells was stimulated by EGF. It seems likely that an autocrine system, in which EGF serves as the signal, may exist in cervical squamous carcinoma. 17beta-estradiol and L-triiodothyronine were found to upregulate EGF-R expression, proliferative potential and SCC production in the CaSki cervical carcinoma cells.     

Anaerobic digestion of alkaline black liquor using an up-flow anaerobic sludge blanket reactor

The anaerobic digestion of alkaline black liquor from a cereal straw pulping mill was studied in batch (serum bottles) and continuous systems (up-flow anaerobic sludge blanket reactor—UASB). The batch digestion studies confirmed that lignin and related compounds (LRC) in the alkaline black liquor were the main inhibitory substances and could not be decomposed by anaerobic bacteria. At organic loading rates of 5–10 kg COD m^?3 day^?1, the UASB reactor achieved 50–60% COD removal efficiencies. Gas production was 2–3 dm³ per dm³ of alkaline black liquor. Two different sludge types were examined in the reactor: granular and cluster-like sludges. Sludge in a cluster, which involved many small granules and flocs, tended to form larger aggregates and possessed good settling ability.     

基于MACH 4002交换机的煤矿网络系统

以MACH 4002交换机为网络核心,分析了煤矿环境对网络系统的实时性、可靠性要求,比较了多种拓扑结构特性,给出了行之有效的建设方案。     

Achieving fair service with a hybrid scheduling scheme for CICQ switches

Providing performance guarantees for arriving traffic flows has become an important measure for today’s routing and switching systems. However, none of current scheduling algorithms built on CICQ (combined input and cross-point buffered) switches can provide flow level performance guarantees. Aiming at meeting this requirement, the feasibility of implementing flow level scheduling is discussed thoroughly. Then, based on the discussion, it comes up with a hybrid and stratified fair scheduling (HSFS) scheme, which is hierarchical and hybrid, for CICQ switches. With HSFS, each input port and output port can schedule variable length packets independently with a complexity of O(1). Theoretical analysis show that HSFS can provide delay bound, service rate and fair performance guarantees without speedup. Finally, we implement HSFS in SPES (switch performance evaluation system) to verify the analytical results.     

An efficient search mechanism for supporting partial filename queries in structured peer-to-peer overlay

Accompanying the growth of the Internet, computers throughout the world can connect to each other and exchange information, increasing the convenience and efficiency of information-based work. The advent of data-sharing applications, such as Napster and Gnutella, has made peer-to-peer (P2P) systems popular for widespread exchange of resources and voluminous information between millions of users. In recent years, research issues associated with P2P systems have been discussed widely. To resolve the file-availability problem and improve the workload, a method called the Distributed Hash Table (DHT) has been proposed. However, DHT-based systems in structured architectures cannot support efficient queries, such as a similarity query, range query, and partial-match query, due to the characteristics of the hash function. This study presents a novel scheme that supports filename partial-matches in structured P2P systems. The proposed approach supports complex queries and guarantees result quality. Experimental results demonstrate the effectiveness of the proposed approach.     

古尔班通古特沙漠梭梭林地NDVI的时间效应-基于物候变化和MODIS图像的研究

遥感图像信息提取研究是遥感研究中的一个关键问题,也是遥感研究的热点和难点之一。使用2000~2010年MODIS-NDVI 16 d合成数据和物候记录,借助GIS空间分析和统计分析方法,重构了古尔班通古特沙漠梭梭林地Mean NDVI时间序列特征曲线。分析物候与Mean NDVI时间序列表明,梭梭林地内的短命植物生长期早于梭梭。研究梭梭林地Mean NDVI时间序列曲线发现,曲线中存在一个明显区别于其他地物的特征点,该点可以作为梭梭林地信息“诊断点”。根据“诊断点”特征构建了梭梭林地特征指数模型(HFFI),进而反演了古尔班通古特沙漠梭梭林地信息,并利用地面实际观测资料进行验证,结果表明分类精度达到83%。     

锡膏激光扫描三维测量系统光强自适应调节技术

根据锡膏激光扫描三维测量系统在测量PCB电路板时底板和锡膏对激光光强的散射效果不同,设计并研制了一套光强自适应调节光源。该光源以程控电源为基础,分析光条中心提取效果与激光光强的关系,应用小波分解与频带能量比相结合的光强自适应调节算法实现测量系统光强自适应调节功能。进行了相关实验,实验结果表明该光源能够很好地实现锡膏激光扫描三维测量系统的激光光强自适应调节,并能选出最适合测量的激光光强,具有很好的适用性。     

基于XML的新闻管理系统设计

简要分析基于XML的新闻管理系统的架构及主要功能模块设计,构建Web Service实现新闻发布旨在实现信息资源共享,并通过案例加以说明。