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31.
32.
LJ Huang K Durick JA Weiner J Chun SS Taylor 《Canadian Metallurgical Quarterly》1997,94(21):11184-11189
Subcellular localization directed by specific A kinase anchoring proteins (AKAPs) is a mechanism for compartmentalization of cAMP-dependent protein kinase (PKA). Using a two-hybrid screen, a novel AKAP was isolated. Because it interacts with both the type I and type II regulatory subunits, it was defined as a dual specific AKAP or D-AKAP1. Here we report the cloning and characterization of another novel cDNA isolated from that screen. This new member of the D-AKAP family, D-AKAP2, also binds both types of regulatory subunits. A message of 5 kb pairs was detected for D-AKAP2 in all embryonic stages and in all adult tissues tested. In brain, skeletal muscle, kidney, and testis, a 10-kb mRNA was identified. In testis, several small mRNAs were observed. Therefore, D-AKAP2 represents a novel family of proteins. cDNA cloning from a mouse testis library identified the full length D-AKAP2. It is composed of 372 amino acids which includes the R binding fragment, residues 333-372, at its C-terminus. Based on coprecipitation assays, the R binding domain interacts with the N-terminal dimerization domain of RIalpha and RIIalpha. A putative RGS domain was identified near the N-terminal region of D-AKAP2. The presence of this domain raises the intriguing possibility that D-AKAP2 may interact with a Galpha protein thus providing a link between the signaling machinery at the plasma membrane and the downstream kinase. 相似文献
33.
Weian Huang Fuhrmann D.R. Politte D.G. Thomas L.J. Jr. States D.J. 《IEEE transactions on bio-medical engineering》1998,45(4):422-428
In four-color fluorescence-based automated DNA sequencing, a 4×4 filter matrix parameterizes the relationship between the dye-intensity signals of interest and the data collected by an optical imaging system. The filter matrix is important because the estimated DNA sequence is based on the dye intensities that can only be recovered via inversion of the matrix. Here, the authors present a calibration method for the estimation of the columns of this matrix, using data generated through a special experiment in which DNA samples are labeled with only one fluorescent dye at a time. Simulations and applications of the method to real data are provided, with promising results 相似文献
34.
35.
本文针对一往复式氢气压缩机管道振动十分强烈的情况,分析了管系的结构自振频率、气柱固有频率和气流脉动,采取了改变管系结构频率和气柱固有频率的减振措施,经实践证明获得了较为理想的减振效果。 相似文献
36.
There is growing evidence that the amino-terminal globular domain of apolipoprotein B (apoB) is essential for lipoprotein particle formation in the hepatic endoplasmic reticulum. To identify the structural requirements for its function in lipoprotein assembly, cysteine (Cys) pairs required to form the seven disulfide bonds within the amino-terminal 21% of apoB were replaced in groups or individually by serine. Substitution of Cys pairs required for formation of disulfide bonds 1-3 or 4-7 (numbered from amino to carboxyl terminus) completely blocked the secretion of apoB28 in transfected HepG2 cells. To identify the specific disulfide bonds required for secretion, Cys pairs were mutated individually. Substitution of Cys pairs required for disulfide bonds 1, 3, 5, 6, or 7 had little or no impact on apoB28 secretion or buoyant density. In contrast, individual substitution of Cys pair 2 (amino acid residues 51 and 70) or 4 (218 and 234) severely inhibited apoB28 secretion and its capacity to undergo intracellular assembly with lipid. The same assembly and secretion defects were observed when these mutations were expressed as part of apoB50. These studies provide direct evidence that the ability of the internal lipophilic regions of apoB to engage in the recruitment and sequestration of lipid during translation is critically dependent upon a structural configuration contained within or affected by the amino-terminal 5% of the protein. 相似文献
37.
数模混合仿真中模拟信号的恢复及其误差对整个仿真过程具有重要影响。本文探讨了用零阶保持和一阶保持进行信号恢复的方法,比较了它们的误差及工作特点。特别指出了一阶保持中,由于存在积分器复原时间及数字机操作时间的影响而会带来附加的误差,因此一阶保持方法较难达到其理论的精度。 相似文献
38.
The magnetization of a melt-texture growth (MTG) HTSC ring has been studied. It is shown that the magnetic field inside the ring is larger than the external field under a certain range of external magnetic fields. We have also investigated the magnetic field dependence of the response of a detective coil near a rotating superconducting ring. The responses of the MTG sample are different for different cooling methods. 相似文献
39.
A Linear Cross-Coupled Control System for High-Speed Machining 总被引:4,自引:0,他引:4
Q. Zhong Y. Shi J. Mo S. Huang 《The International Journal of Advanced Manufacturing Technology》2002,19(8):558-563
We present a linear cross-coupled controller to improve highspeed contouring accuracy independently of tracking accuracy in
a biaxial machine tool feed drive servomechanism. Unlike conventional cross-coupled controllers, the cross-coupled controller
presented here is a linear system, so it is very easy to perform the stability and steady-state error analysis, and to optimise
the controller parameters. The proposed controller is evaluated experimentally on a CNC LOM machine and compared to an uncoupled
controller and a conventional cross-coupled controller. Controller performance is evaluated for a circular contour at a feedrate
of 30 m min _1 . The experimental results show that the proposed controller can greatly reduce the contour error at large feedrates. The
linear cross-coupled controller is simple to implement and is practical. 相似文献
40.
T. T. Wang T. H. Huang 《Tunnelling and Underground Space Technology incorporating Trenchless Technology Research》2002,17(4):425-436
Many tunnels will be constructed in southwestern Taiwan in the upcoming decade to meet the huge demands of transportation, energy and water infrastructure projects. Mudstone strata cover more than a 1000 km2 area, consisting mainly of massive mudstone or alternation of mudstone and sandy layer, exhibiting unfavorable geological and hydrological characteristics, making it extremely difficult for tunnelling. This paper presents the lessons learned from three tunnelling projects in the 1990s in a mudstone area. In addition to discussing the rock behavior in the vicinity of tunnel and its failure patterns, the monitoring data during tunnelling are presented as well. Finally, the effective method of design and construction are recommended for tunnelling in mudstone area. 相似文献