淀粉分子磁化修饰及其再生膜材料的制备

以高直链淀粉(HS)为原料,采用"还原胺化-点击化学法"制备得到具有磁性的变性淀粉(MMS),并在外加磁场作用下制备再生淀粉膜.具体包括在HS分子惟一的潜在醛基上引入炔基,对纳米Fe3O4粒子(MNP)进行硅烷包覆得到叠氮化磁体粒子(azide-MNPs),两者经点击化学反应得到分子链末端带磁性的变性淀粉.借助XRD、FTIR、NMR和MS对产物结构进行表征.结果表明,磁体粒子精准接枝到高直链淀粉分子还原末端,得到目标产物MMS.再生淀粉膜性能测试结果表明,在外磁场诱导条件下MMS逐渐趋于分子有序排列,在MMS质量分数为15％,4×104 A/m磁场作用下制得的再生淀粉膜较纯羧甲基淀粉膜断裂伸长率提升49.5％,断裂强度提升88.3％,短程有序程度提高.     

110kV电缆终端红外测温培训系统开发

红外测温作为一项非接触式带电检测技术,可快速、安全地发现电缆终端异常发热缺陷.为了相应地提高巡检人员红外拍摄技能水平和图像分析能力,在总结归纳各类电缆终端故障案例特点的基础上,设计了一种110 kV电缆终端红外测温培训系统,该系统等比还原了110 kV电缆终端.内部结构采用110 kV终端样品和加热系统相结合,可真实模拟电缆终端运行工况;布置多个定制加热器及温控表,灵活配置发热状态;采用PID控温算法,使温度控制平稳;采用ModBus-Tcp协议与温控表通信,通过WiFi-485总线实现移动终端对温控箱的远程设置;人机交互界面可灵活设置温度并实时显示监测结果.该系统在实训中取得良好效果.     

多颜色粉末涂料在汽车车厢上的开发应用

介绍了粉末涂料的喷涂工艺流程、喷涂设备及烘干系统,对多颜色串色问题提出了解决方案,制定了汽车车厢用粉末涂料的技术指标,并举例说明了验证粉末涂料与前处理电泳防腐配套性能的方法.     

粉煤灰空心微珠强化厚型钢结构防火涂料的制备及性能

将小粒径粉煤灰空心微珠(FAC)作为隔热填料掺入厚型钢结构防火涂料中,研究了FAC掺量对涂料理化性能及耐火性能的影响.结果显示:FAC掺量为8％时得到的防火涂料整体性能最优,灼烧3 h的背温低于市售防火涂层.利用数据模型对防火涂料耐火试验过程参数进行计算,求得固定和平均这2种形式的等效热阻,再以其模拟钢构件受火过程的温升情况,结果与实测基本一致.探讨了涂层表现出耐火性的原因.     

三维微组装多气体传感器测试仪

针对在地震等自然灾害及遮蔽空间范围传感器体积受限制和对周围环境参数感知问题,设计了三维微组装多气体传感器测试仪,结合设计的GPSO-BP算法,该测试仪可以在高温高湿的环境下实现实时对有毒气体浓度以及压力的多参数测量,并对其进行温湿度补偿,具有高性能、小型化、准确度高的特点.测试结果表明,在湿度为80％RH,温度为50℃的环境下测量不同气体,经过温湿度补偿后,该测试仪的准确对度至少提高到2.2％以上,稳定性较强,对于地震等恶劣环境下的环境参数感知具有重大的意义.     

基于灰色关联分析的电网安全事故关键致因分析

探讨和研究电网安全事故的影响因素及控制已成为预防电网安全事故发生的热点问题。为揭示电网安全事故发生的深层机理,基于事故致因理论,采用质性分析和灰色关联分析的方法,分析中国电网安全事故的关键致因。研究发现,电网安全事故的影响因素主要包括主体因素（认知因素、行为因素和身体因素）、过程因素（组织氛围因素和组织过程因素）、对象因素（设备可靠性低和设备管理松懈）及情境因素（作业环境因素和非作业环境因素）。灰色关联分析表明：电网安全事故的致因因素可以分为红色危险因素（r≥0.8）、橙色警备因素（0.7≤r＜0.8）和黄色警告因素（r＜0.7）,其中,组织氛围因素、认知因素和行为因素是电网安全事故的关键致因因素。在此基础上提出电网安全事故管理的政策建议,以期推动中国电网安全发展进程。     

开关类设备局部放电光脉冲与特高频脉冲特性对比研究

探究了新型硅光电传感器在局部放电检测方面的性能,并将其测得的光脉冲与传统特高频脉冲进行了对比试验分析。通过光、电同步局放试验,对各种传感器的检测性能、工作特性和统计特征进行了分析和讨论,探索了硅光电局放传感器在实际放电监测中的优势及可行性。此外,通过光、电同步监测数据分析,获得了局放光辐射与电磁辐射两种物理现象的统计学表现。结果表明,与特高频传感器相比,处在最佳工作电压下的硅光电传感器有着更高的信噪比;两种光学检测手段在高频电机的电磁干扰下,硅光电传感器体现出更好的抗电磁干扰能力;两种检测手段获得的PRPD图谱所反映的放电相位区间与放电特征都保持较好的一致性;脉冲重复率随施加电压的变化趋势一致,但硅光电传感器具有较高的信噪比和单光子灵敏度,得到的光脉冲统计频次相对局放电磁脉冲频次较高, 并且相基统计数据中的偏斜度和陡峭度也具有较好的一致性。     

Apoptotic Effects of Anthocyanins from Vitis coignetiae Pulliat Are Enhanced by Augmented Enhancer of the Rudimentary Homolog (ERH) in Human Gastric Carcinoma MKN28 Cells

Evidence suggests that augmented expression of a certain gene can influence the efficacy of targeted and conventional chemotherapies. Here, we tested whether the high expression of enhancer of the rudimentary homolog (ERH), which serves as a prognostic factor in some cancers, can influence the efficacy of anthocyanins isolated from fruits of Vitis coignetiae Pulliat, Meoru in Korea (AIMs) on human gastric cancer cells. The anticancer efficacy of AIMs was augmented in ERH-transfected MKN28 cells (E-MKN28 cells). Molecularly, ERH augmented AIM-induced caspase-dependent apoptosis by activating caspase-3 and -9. The ERH-augmented apoptotic effect was related to mitochondrial depolarization and inhibition of antiapoptotic proteins, XIAP, and Bcl-2. In addition, reactive oxygen species (ROS) generation was augmented in AIMs-treated E-MKN28 cells compared to AIMs-treated naïve MKN28 cells. In conclusion, ERH augmented AIM-induced caspase-dependent mitochondrial-related apoptosis in MKN28 cells. A decrease in expression of Bcl-2 and subsequent excessive ROS generation would be the mechanism for ERH-augmented mitochondrial-related apoptosis in AIMs-treated MKN28 cells. A decrease in expression of XIAP would be another mechanism for ERH-augmented caspase-dependent apoptosis in AIMs-treated MKN28 cells.     

Soluble Prokaryotic Overexpression and Purification of Human GM-CSF Using the Protein Disulfide Isomerase b′a′ Domain

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is a member of the colony-stimulating factor (CSF) family, which functions to enhance the proliferation and differentiation of hematopoietic stem cells and other hematopoietic lineages such as neutrophils, dendritic cells, or macrophages. These proteins have thus generated considerable interest in clinical therapy research. A current obstacle to the prokaryotic production of human GM-CSF (hGM-CSF) is its low solubility when overexpressed and subsequent complex refolding processes. In our present study, the solubility of hGM-CSF was examined when combined with three N-terminal fusion tags in five E. coli strains at three different expression temperatures. In the five E. coli strains BL21 (DE3), ClearColi BL21 (DE3), LOBSTR, SHuffle T7 and Origami2 (DE3), the hexahistidine-tagged hGM-CSF showed the best expression but was insoluble in all cases at each examined temperature. Tagging with the maltose-binding protein (MBP) and the b′a′ domain of protein disulfide isomerase (PDIb′a′) greatly improved the soluble overexpression of hGM-CSF at 30 °C and 18 °C. The solubility was not improved using the Origami2 (DE3) and SHuffle T7 strains that have been engineered for disulfide bond formation. Two conventional chromatographic steps were used to purify hGM-CSF from the overexpressed PDIb′a′-hGM-CSF produced in ClearColi BL21 (DE3). In the experiment, 0.65 mg of hGM-CSF was isolated from a 0.5 L flask culture of these E. coli and showed a 98% purity by SDS-PAGE analysis and silver staining. The bioactivity of this purified hGM-CSF was measured at an EC₅₀ of 16.4 ± 2 pM by a CCK8 assay in TF-1 human erythroleukemia cells.     

Activation of Nrf2/HO-1 by Peptide YD1 Attenuates Inflammatory Symptoms through Suppression of TLR4/MYyD88/NF-κB Signaling Cascade

In this study, we investigate the immunomodulatory effects of a novel antimicrobial peptide, YD1, isolated from Kimchi, in both in vitro and in vivo models. We establish that YD1 exerts its anti-inflammatory effects via up-regulation of the Nrf2 pathway, resulting in the production of HO-1, which suppresses activation of the NF-κB pathway, including the subsequent proinflammatory cytokines IL-1β, IL-6, and TNF-α. We also found that YD1 robustly suppresses nitric oxide (NO) and prostaglandin E2 (PGE₂) production by down-regulating the expression of the upstream genes, iNOS and COX-2, acting as a strong antioxidant. Collectively, YD1 exhibits vigorous anti-inflammatory and antioxidant activity, presenting it as an interesting potential therapeutic agent.