(+)-CC-1065 as a structural probe of Mu transposase-induced bending of DNA: overcoming limitations of hydroxyl-radical footprinting

Phage Mu transposase (A-protein) is primarily responsible for transposition of the Mu genome. The protein binds to six att sites, three at each end of Mu DNA. At most att sites interaction of a protein monomer with DNA is seen to occur over three minor and two consecutive major grooves and to result in bending up to about 90 degrees. To probe the directionality and locus of these A-protein-induced bends, we have used the antitumor antibiotic (+)-CC-1065 as a structural probe. As a consequence of binding within the minor groove, (+)-CC-1065 is able to alkylate N3 of adenine in a sequence selective manner. This selectivity is partially determined by conformational flexibility of the DNA sequence, and the covalent adduct has a bent DNA structure in which narrowing of the minor groove has occurred. Using this drug in experiments in which either gel retardation or DNA strand breakage are used to monitor the stability of the A-protein--DNA complex or the (+)-CC-1065 alkylation sites on DNA (att site L3), we have demonstrated that of the three minor grooves implicated in the interaction with A-protein, the peripheral two are 'open' or accessible to drug bonding following protein binding. These drug-bonding sites very likely represent binding at at least two A-protein-induced bending sites. Significantly, the locus of bending at these sites is spaced approximately two helical turns apart, and the bending is proposed to occur by narrowing of the minor groove of DNA. The intervening minor groove between these two peripheral sites is protected from (+)-CC-1065 alkylation. The results are discussed in reference to a proposed model for overall DNA bending in the A-protein att L3 site complex. This study illustrates the utility of (+)-CC-1065 as a probe for protein-induced bending of DNA, as well as for interactions of minor groove DNA bending proteins with DNA which may be masked in hydroxyl radical footprinting experiments.     

PbO－Bi_2O_3－B_2O_3－SiO_2系重金属氧化物玻璃的结构与光学性

通过密度、可见光光谱、红外吸收光谱、Ｃｏ－６０辐照损伤试验及荧光光谱的测试，研究了ＰｂＯ－Ｂｉ２Ｏ３－Ｂ２Ｏ３－ＳｉＯ２玻璃系的光学性能与结构．密度最高可达８．４６４ｇ／ｃｍ３其紫外吸收达截止波长随Ｐｂ２＋及Ｂｉ３＋含量升高而红移．玻璃熔化温度低达８５０℃．在ＰｂＯ－Ｂｉ２Ｏ３－Ｂ２Ｏ３系玻璃中加人ＳｉＯ２可使玻璃结构更致密．室温下该系统玻璃在３６０ｎｍ有一个宽的激发峰，能产生４１８ｕｍ及４３８ｕｍ两个弱的发射峰．该系统玻璃的结构是由［ＳｉＯ４］４－、［ＢＯ３］３－、［ＢＯ４］５－、［ＰｂＯ４］６－及［ＢｉＯ６］９－构成．其中部分Ｐｂ２＋及Ｂｉ３＋以网络外体进入玻璃．     

Experimental studies of new ductile coupling beams and multi-storey shear walls

The span-depth ratios of coupling beams to interconnect shear walls are generally small, so that brittle shear failure may occur and lead to reduced ductility. In order to improve the ductility of coupling beams for earthquake loading, a new type of ductile coupling beam is proposed in this paper. Along the middle depth of this beam, a slit through the entire thickness (a narrow hole) near each end and two lateral keyways along the remaining middle part of the span are made. The reductions of the stiffness under service load and the ultimate carrying capacity of the beam due to the weakening mentioned above are small and the ductility is greatly increased. Tests of 4-storey walls interconnected by coupling beams in three different constructions (monolithic beams, beams with a through-slit and new-type beams) indicated that shear walls with the new coupling beams possess the best aseismic behaviours under cyclic loading. The proposed new coupling beam has been used in a high-rise building.     

正庚烷-甲苯-三甘醇三元体系液-液相平衡数据的测定与关联

利用气相色谱法测定了常压下２５℃、４０℃和５０℃时正庚烷－甲苯－三甘醇体系的液－液相平衡数据，采用峰面积归一化法定量，所得数据准确、可靠。用ＮＲＴＬ模型对所测数据进行了热力学关联，求出了相应的模型参数。用该模型对本三元体系进行了预测，结果令人满意。     

几种新型多波长光纤激光器

综合报道几种新型多波长光纤激光器 ,并对这些激光器的机理、实验装置和研究结果进行了详细介绍 ,对今后多波长激光器的发展方向作一展望。     

过滤技术在煤炭工业中的应用

一．前言 我国现有的洗煤厂，绝大部分都是采用湿法（以水或重悬浮液作为分选介质）选煤技术进行煤炭的分选。产品脱水就成为洗煤生产中的一个突出问题，对于洗煤厂来说，产品脱水也是一项保证产品质量，节约用水，节约运力，节省动力和减少污染的至关重要的工作。     

森林火情监测预报研究

对现行的森林火情监测方法进行了分析比较。根据实际存在的难题，提出了料为产用的实施方案及应采取的技术措施，进行了实地试验，取得较好的效果。     

激光光束质量因子的研究进展

本文详细分析了各种光场下光束质量M2因子。在不同的场中,A.E.Siegman当初所定义的M2因子,已经有了很大的变化,因而M2因子的特性也与当初不同,不仅M2因子可以大于等于1,而且可以小于1,甚至小于0。其反映的物理意义,也从经典场中的衍射极限倍数,变化为非经典场中的准直性量度以及对空间相干性的某些反映。此外还列举并分析了几种特殊的光束质量因子,最后讨论了M2因子应用的问题并指出了其局限性。     

风雨操场综合楼改造工程设计与施工

某风雨操场综合楼改造工程拆除了辅助用房及支撑网架屋面的西根框架柱改造成为球场看台。设计采用钢结构桁架托换技术，将屋面荷载转移至两侧的保留柱上，跨度拓展到30m。钢桁架与框架柱上安装的牛腿铰接连接，通过牛腿背面框架柱间的混凝土墙平衡牛腿传来的弯矩。在施工安装过程中合理地利用待拆除框架柱，保持屋面的完整性提升屋面，达到了对屋面扰动影响最小的目的。