全文获取类型
收费全文 | 24274篇 |
免费 | 2755篇 |
国内免费 | 1826篇 |
专业分类
电工技术 | 2168篇 |
综合类 | 2719篇 |
化学工业 | 2877篇 |
金属工艺 | 1510篇 |
机械仪表 | 1701篇 |
建筑科学 | 1700篇 |
矿业工程 | 1002篇 |
能源动力 | 715篇 |
轻工业 | 2618篇 |
水利工程 | 858篇 |
石油天然气 | 722篇 |
武器工业 | 417篇 |
无线电 | 2659篇 |
一般工业技术 | 1861篇 |
冶金工业 | 1641篇 |
原子能技术 | 320篇 |
自动化技术 | 3367篇 |
出版年
2024年 | 154篇 |
2023年 | 362篇 |
2022年 | 958篇 |
2021年 | 1190篇 |
2020年 | 864篇 |
2019年 | 620篇 |
2018年 | 672篇 |
2017年 | 743篇 |
2016年 | 675篇 |
2015年 | 1070篇 |
2014年 | 1355篇 |
2013年 | 1610篇 |
2012年 | 2006篇 |
2011年 | 2017篇 |
2010年 | 2063篇 |
2009年 | 1831篇 |
2008年 | 1921篇 |
2007年 | 1853篇 |
2006年 | 1590篇 |
2005年 | 1246篇 |
2004年 | 884篇 |
2003年 | 604篇 |
2002年 | 496篇 |
2001年 | 544篇 |
2000年 | 371篇 |
1999年 | 149篇 |
1998年 | 291篇 |
1997年 | 176篇 |
1996年 | 127篇 |
1995年 | 61篇 |
1994年 | 50篇 |
1993年 | 56篇 |
1992年 | 22篇 |
1991年 | 25篇 |
1990年 | 20篇 |
1989年 | 25篇 |
1988年 | 19篇 |
1987年 | 16篇 |
1986年 | 9篇 |
1985年 | 4篇 |
1984年 | 3篇 |
1983年 | 4篇 |
1982年 | 5篇 |
1981年 | 5篇 |
1980年 | 12篇 |
1978年 | 2篇 |
1977年 | 18篇 |
1976年 | 38篇 |
1959年 | 10篇 |
1951年 | 7篇 |
排序方式: 共有10000条查询结果,搜索用时 15 毫秒
61.
T Kimura I Hashimoto M Nishikawa JI Fujisawa 《Canadian Metallurgical Quarterly》1996,78(11-12):1075-1080
Human immunodeficiency virus type-1 (HIV-1) Rev acts by inducing the specific nucleocytoplasmic transport of a class of incompletely spliced RNAs that encodes the viral structural proteins. The transfection of HeLa cells with a rev-defective HIV-1 expression plasmid, however, resulted in the export of overexpressed, intron-containing species of viral RNAs, possibly through a default process of nuclear retention. Thus, this system enabled us to directly compare Rev+ and Rev+ cells as to the usage of RRE-containing mRNAs by the cellular translational machinery. Biochemical examination of the transfected cells revealed that although significant levels of gag and env mRNAs were detected in both the presence and absence of Rev, efficient production of viral proteins was strictly dependent on the presence of Rev. A fluorescence in situ hybridisation assay confirmed these findings and provided further evidence that even in the presence of Rev, not all of the viral mRNA was equally translated. At the early phase of RNA export in Rev+ cells, gag mRNA was observed throughout both the cytoplasm and nucleoplasm as uniform fine stippling. In addition, the mRNA formed clusters mainly in the perinuclear region, which were not observed in Rev+ cells. In the presence of Rev, expression of the gag protein was limited to these perinuclear sites where the mRNA accumulated. Subsequent staining of the cytoskeletal proteins demonstrated that in Rev+ cells gag mRNA is colocalized with beta-actin in the sites where the RNA formed clusters. In the absence of Rev, in contrast, the gag mRNA failed to associate with the cytoskeletal proteins. These results suggest that in addition to promoting the emergence of intron-containing RNA from the nucleus, Rev plays an important role in the compartmentation of translation by directing RRE-containing mRNAs to the beta-actin to form the perinuclear clusters at which the synthesis of viral structural proteins begins. 相似文献
62.
63.
JI Haring 《Canadian Metallurgical Quarterly》1998,18(5):14-6, 58
64.
目的改良大鼠可逆性大脑中动脉缺血模型的栓子制作方法,增加模型稳定性和成功率,提高脑缺血实验研究结果的可靠性.方法体重280~320g Wistar雄性大鼠60只,随机分成改良组和对照组,每组30只.对照组用尼龙线一端加热法制作传统栓子,改良组用尼龙线一端沾silicone胶法制作改良栓子.两组模型除栓子制作方法不同外,其他手术步骤完全相同.采用Zea Longa 5分制法进行模型的神经功能评分,采用TTC染色法测量并计算梗死灶的大小.比较两组模型制作成功率、成功模型中神经功能评分3、4级大鼠所占百分数,以 相似文献
65.
介绍一种当应力、强度呈非正态分布时的可靠度的计算方法,以用来进行机械可靠性设计,并以一个地震载荷的直立压力容器为例加以说明。 相似文献
66.
克拉玛依原油中各组分的乳化性质 总被引:3,自引:0,他引:3
用沉淀法和色谱分离法将克拉玛依原油分离成沥青质,极性物和抽余油三组分,红外光谱分析表明,沥青质和极性物中存在一些含氧官能团的羧酸类或酚类以及含氮化合物,而抽余油则主要为烃类物质,因而沥青质和极性物的界面活性大于抽余油,沥青质具有较强的油包水(W/O)乳化能力,是造成克拉玛依原油W/O乳化的主要组分,极性物具有较强的水包油(O/W)乳化能力,是造成克拉玛依原油O/W乳化的主要组分,抽余油的乳化能力很弱。 相似文献
67.
68.
69.
PURPOSE: This study investigated the pathogenesis of tractional retinal detachment associated with proliferative vitreoretinopathy in an experimental model, using immunohistochemical staining. METHODS: To produce tractional retinal detachment in rabbit eyes, homologous cultured fibroblasts obtained from the gluteal muscle fascia were injected intravitreously. Right eyes of 20 rabbits in the study group, and 7 rabbits in the control group were followed for 26 days at weekly intervals with indirect ophthalmoscopy and fundus photographs. RESULTS: During the follow-up period grade III tractional retinal detachment developed in 11 eyes, grade II in six, and grade 1 in three eyes. The spindle-shaped cells contributed predominantly to the development of epiretinal membrane, and a smaller number of round small and large cells. In 10/17 grade II and III eyes, spindle-shaped cells had vimentin, 7/10 had actin, 5/17 had GFAP, 4/17 had S-100 protein immunoreactivity. Round small and large cells expressed S-100 protein, GFAP and actin in 5/17 eyes. Epiretinal membrane appeared to be formed by spindle-shaped fibroblast-like cells and small and large round glia-like cells. Actin positivity of spindle-shaped and round cells was taken as a marker of contractile elements of the cells and their locomotional features. CONCLUSIONS: These features are believed to be involved in contraction of the membrane and retinal detachment. 相似文献
70.
O van der Heijden HC Chiu TC Park H Takahashi VA LiVolsi JI Risinger JC Barrett A Berchuck AC Evans K Behbakht AW Menzin PC Liu I Benjamin MA Morgan SA King SC Rubin J Boyd 《Canadian Metallurgical Quarterly》1998,23(4):243-247
The degree and nature of patient involvement in consultations with health professionals influences problem and needs recognition and management, and public accountability. This paper suggests a framework for understanding the scope for patient involvement in such consultations. Patients are defined as co-producers of formal health services, whose potential for involvement in consultations depends on their personal rights, responsibilities and preferences. Patients' rights in consultations are poorly defined and, in the National Health Service (NHS), not legally enforceable. The responsibilities of patients are also undefined. I suggest that these are not to deny, of their own volition, the rights of others, which in consultations necessitate mutuality of involvement through information-exchange and shared decision-making. Preferences should be met insofar as they do not militate against responsibilities and rights. 相似文献