Local Epidermal Endocrine Estrogen Protects Human Melanocytes against Oxidative Stress,a Novel Insight into Vitiligo Pathology

As the outermost barrier of the body, skin is a major target of oxidative stress. In the brain, estrogen has been reported synthesized locally and protects neurons from oxidative stress. Here, we explored whether estrogen is also locally synthesized in the skin to protect from oxidative stress and whether aberrant local estrogen synthesis is involved in skin disorders. Enzymes and estrogen receptor expression in skin cells were examined first by quantitative real-time PCR and Western blot analyses. Interestingly, the estrogen synthesis enzyme was mainly localized in epidermal keratinocytes and estrogen receptors were mainly expressed in melanocytes among 13 kinds of cultured human skin cells. The most abundant estrogen synthesis enzyme expressed in the epidermis was 17β-hydroxysteroid dehydrogenase 1 (HSD17β1) localized in keratinocytes, and the most dominant estrogen receptor expressed in the epidermis was G protein-coupled estrogen receptor 1 (GPER1) in melanocytes. To investigate whether keratinocyte-derived estradiol could protect melanocytes from oxidative stress, cultured human primary epidermal melanocytes (HEMn-MPs) were treated with H₂O₂ in the presence or absence of 17β estradiol or co-cultured with HSD17β1 siRNA-transfected keratinocytes. Keratinocyte-derived estradiol exhibited protective effects against H₂O₂-induced cell death. Further, reduced expression of HSD17β1 in the epidermis of skin from vitiligo patients was observed compared to the skin from healthy donors or in the normal portions of the skin in vitiligo patients. Our results suggest a possible new target for interventions that may be used in combination with current therapies for patients with vitiligo.     

Cytological Observations and Bulked-Segregant Analysis Coupled Global Genome Sequencing Reveal Two Genes Associated with Pollen Fertility in Tetraploid Rice

Neo-tetraploid rice with high fertility is a useful germplasm for polyploid rice breeding, which was developed from the crossing of different autotetraploid rice lines. However, little information is available on the molecular mechanism underlying the fertility of neo-tetraploid rice. Here, two contrasting populations of tetraploid rice, including one with high fertility (hereafter referred to as JG) and another with low fertility (hereafter referred to as JD), were generated by crossing Huaduo 3 (H3), a high fertility neo-tetraploid rice that was developed by crossing Jackson-4x with 96025-4x, and Huajingxian74-4x (T452), a low fertility autotetraploid rice parent. Cytological, global genome sequencing-based bulked-segregant (BSA-seq) and CRISPR/Cas9 technology were employed to study the genes associated with pollen fertility in neo-tetraploid rice. The embryo sacs of JG and JD lines were normal; however, pollen fertility was low in JD, which led to scarce fertilization and low seed setting. Cytological observations displayed low pollen fertility (25.1%) and approximately 31.3 and 27.2% chromosome lagging at metaphase I and II, and 28.8 and 24.8% chromosome straggling at anaphase I and II in JD, respectively. BSA-seq of F_2–3 generations and RNA-seq of F₄ generation detected a common fragment, i.e., 18,915,234–19,500,000, at chromosome 7, which was comprised of 78 genes associated with fertility. Among 78 genes, 9 genes had been known to be involved in meiosis and pollen development. Two mutants ny1 (LOC_Os07g32406) and ny2 (LOC_Os07g32040) were generated by CRISPR/Cas9 knockout in neo-tetraploid rice, and which exhibited low pollen fertility and abnormal chromosome behavior. Our study revealed that two unknown genes, LOC_Os07g32406 (NY1) and LOC_Os07g32040 (NY2) play an important role in pollen development of neo-tetraploid rice and provides a new perspective about the genetic mechanisms of fertility in polyploid rice.     

关于氢综合利用系统在西安地区宾馆类场景中的配置方法研究

氢综合利用技术可为电网的削峰填谷起到积极作用,有利于改善系统负荷日曲线。根据宾馆类用电特性,采用减小峰谷差率法和利用峰谷电价差法两种方法配置氢综合利用系统。从用户自身角度,利用峰谷电价差法配置氢综合利用系统经济性更优;从电网角度,采用减小峰谷差率法配置氢综合利用系统可增加全社会综合效益。从电网和用户均受益的角度出发,一是建议结合宾馆类场景的负荷特性,调节峰谷差电价;二是建议对氢综合利用系统进行技术创新,降低其成本,减小成本回收时间。     

一种面向弱纹理图像的特征点描述子

现有的大多数特征点提取算法适用于处理纹理丰富的图像,而对于弱纹理图像则无法提取有效的特征点. 对此,提出了多邻域结构张量特征(MNSTF)算法. 基于一系列固定的邻域和图像结构张量,通过表达局部图像的结构和纹理信息,解决了弱纹理和无纹理场景下特征点提取和匹配等相关问题;同时,通过计算邻域之间的相对方向,实现了MNSTF算法特征描述子的旋转不变性. 实验结果表明,MNSTF算法在经过旋转的弱纹理图像测试集上的特征点匹配准确率达到了99.9%以上,验证了其良好的适用性、旋转不变性和鲁棒性.     

"三全育人"理念下高职学生职业素养培育路径研究

通过解析高职学生职业素养的构成要素,分析高职学生职业素养的现状问题,有针对性地探索基于"三全育人"理念的高职学生职业素养培育路径,创新高职学生职业素养的培育模式、工作机制和培养方案,从而推动高职学生职业素养的整体提升.     

基于移动互联的继电保护移动作业平台设计

文章利用移动互联技术,通过搭建"移动终端-主站"软硬件平台,将传统继电保护作业中涉及的文本编辑、业务流转、作业审核、安全监督、资料整理等作业环节进行数字化改造,通过开发作业文本管理模块、安措票管理模块、设备检修缺陷管理模块,实现移动作业终端与现场作业的无缝衔接.继电保护从业人员可通过主站联动方式,提升继电保护作业的工作效率和智能化水平.     

终轧温度和卷取温度对51CrV4热轧板组织性能的影响研究

利用光学显微镜、扫描电镜、Z150电子拉伸试验机分析了不同终轧温度、卷取温度下51CrV4热轧板的显微组织和力学性能。     

X射线衍射-拉伸法测薄膜应力

薄膜应力的测量一直是一个困难的问题。本文尝试用一种新的方法——X射线衍射-拉伸法,对薄膜的应力进行检测,与侧倾法实验结果相比较,X射线衍射-拉伸法更为准确与可靠。     

疲劳裂纹扩展行为的研究现状及钛合金的疲劳裂纹扩展特征

疲劳裂纹扩展行为是现代材料研究中重要的内容之一。论述了组织结构、环境温度、腐蚀条件以及载荷应力比、频率变化对材料疲劳裂纹扩展行为的影响。总结出疲劳裂纹扩展研究的常用方法和理论模型,并讨论了"塑性钝化模型"和"裂纹闭合效应"与实际观察结果存在的矛盾。最后,对钛合金疲劳裂纹扩展研究的内容和研究结果进行了概述。     

Experimental investigation of liquid water in flow field of proton exchange membrane fuel cell by combining X-ray with EIS technologies

The flow field is a pivotal part to manage the transport of water and gas in proton exchange membrane fuel cell. However, the reported water measurement methods (e.g., X-ray and electrochemical impedance spectroscopy (EIS)) cannot give a comprehensive understanding water distribution in the flow field, resulting in challenges in optimizing the channel design and enhancing fuel cell performance. Therefore, we propose a water measurement method combining the X-ray radiography with EIS to investigate the effect of different operating conditions on the growth law and distribution of liquid water in parallel and serpentine flow fields. The attenuation coefficient of liquid water to X-ray is calibrated with constant tube-current and tube-voltage of X-ray generator. Besides, the parallel flow field with hydrophobic treatment is studied. The results show that the water accumulation of the parallel flow field is far more than the serpentine flow field, and the water content of the middle region is higher than that of other regions in the parallel flow field. Furthermore, operating conditions (cathode inlet gas flow rate, inlet gas humidity, and back pressure) have little effect on the liquid water content of the middle region in the parallel flow field. The polarization curve, EIS result, and X-ray radiography show that the performance and water drainage capacity of the hydrophobic parallel flow field are better than the normal one.