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131.
Bengü Ergüden 《Yeast (Chichester, England)》2019,36(2):99-105
The correct separation of chromosomes during mitosis is necessary to prevent genetic instability and aneuploidy, which are responsible for cancer and other diseases, and it depends on proper centrosome duplication. In a recent study, we found that Smy2 can suppress the essential role of Mps2 in the insertion of yeast centrosome into the nuclear membrane by interacting with Eap1, Scp160, and Asc1 and designated this network as SESA (S my2, E ap1, S cp160, A sc1). Detailed analysis showed that the SESA network is part of a mechanism which regulates translation of POM34 mRNA. Thus, SESA is a system that suppresses spindle pole body duplication defects by repressing the translation of POM34 mRNA. In this study, we performed a genome-wide screening in order to identify new members of the SESA network and confirmed Dhh1 as a putative member. Dhh1 is a cytoplasmic DEAD-box helicase known to regulate translation. Therefore, we hypothesized that Dhh1 is responsible for the highly selective inhibition of POM34 mRNA by SESA. 相似文献
132.
The red grape berry pigments, anthocyanins, were characterized in the early 20th century, but investigations of wine pigments were stymied during that era. The question of their identity was a major challenge for wine chemists. A number of techniques showed that the pigments were polymeric in nature. Some structures were postulated by mid-century based on reactions between anthocyanins and condensed tannin, and later postulated wine pigments were observed in model reactions. Some related compounds were then observed in wine. By the end of the 20th century, the ionization of non-volatiles for mass spectrometry opened the door to these compounds. In addition, a new class of compounds was observed, the pyranoanthocyanins, a product of fermentation and aging metabolites with anthocyanins. These compounds possess the pigment stability to SO2 and pH change that is characteristic of aged red wine. Aging experiments show a dynamic situation with shifts in the population of pigment classes over time. The very large number of diverse pigments explains why it has been so difficult to answer the century-old question of the structure of wine pigments. Our current understanding is founded on the use of mass spectral analysis using electrospray and related ionization techniques over the last 25 years. Future progress will rely on more sophisticated analysis of this very complex mixture of substances. © 2019 Society of Chemical Industry 相似文献
133.
Y.K. Yang F.L. Liu Y.W. Zhang M.F. Li F. Ling H.T. Wu 《Ceramics International》2018,44(11):12238-12244
In this work, ultra-low loss Li2MgTi0.7(Mg1/3Nb2/3)0.3O4 ceramics were successfully prepared via the conventional solid-state method. X-ray photoelectron spectroscopy (XPS), thermally stimulated depolarization current (TSDC) and bond energy were used to determine the distinction between intrinsic and extrinsic dielectric loss in (Mg1/3Nb2/3)4+ ions substituted ceramics. The addition of (Mg1/3Nb2/3)4+ ions enhances the bond energy in unit cell without changing the crystal structure of Li2MgTiO4, which results in high Q·f value as an intrinsic factor. The extrinsic factors such as porosity and grain size influence the dielectric loss at lower sintering temperature, while the oxygen vacancies play dominant role when the ceramics densified at 1400?°C. The Li2MgTi0.7(Mg1/3Nb2/3)0.3O4 ceramics sintered at 1400?°C can achieve an excellent combination of microwave dielectric properties: εr =?16.19, Q·f?=?160,000?GHz and τf =??3.14?ppm/°C. In addition, a certain amount of LiF can effectively lower the sintering temperature of the matrix, and the Li2MgTi0.7(Mg1/3Nb2/3)0.3O4-3?wt% LiF ceramics sintered at 1100?°C possess balanced properties with εr?=?16.32, Q·f?=?145,384?GHz and τf =??16.33?ppm/°C. 相似文献
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四川盆地作为国内页岩气开发的重点区域,通过近几年的不断攻关和实践,机械钻速得到了提高,但由于受地层复杂、可钻性差、非均质性强等地质因素的影响,导致井下钻柱系统不良振动剧烈,容易出现钻头损坏严重、钻速较低等问题,严重影响了钻井时效。为了解决上述难题,以该盆地涪陵工区上二叠统龙潭组—中二叠统茅口组为例,采用井下振动高频测量工具的实测手段,测量了钻头—钻柱系统的动态振动加速度参数,结合地层的岩性和矿物组分分析,研究钻头失效原因与对策,并开展了现场试验。研究结果表明:①在非均质地层中钻进的钻头—钻柱系统产生了大于40 m/s~2的高幅值瞬时冲击振动,高幅值的瞬时冲击是导致钻头先期失效的主要原因;②提出了抑制高幅值的瞬时冲击振动采用"减振+增压"工具组合和避免井下工具共振的钻井参数;③采用钻井新参数的试验井比邻井的高幅值瞬时振动降低了17%,单只钻头进尺增加24%,钻头工作环境得到了较大的改善,钻头使用数量减少。结论认为,该研究成果能够有效地改善钻头—钻柱系统的振动状态,有利于达成延长钻头使用寿命的目标。 相似文献
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138.
A new constitutive equation of thermoelasticity for crystals is presented based on the interatomic potential and solid mechanics at finite temperature. Using the new constitutive equation, the calculations for crystal copper and graphene are carried out under different loading paths at different temperatures. The calculated results are in good agreement with those of the previous thermoelasticity constitutive equation based on quantum mechanics, which clearly indicates that our new constitutive equation of thermoelasticity is correct. A lot of comparisons also show that the present theory is more concise and efficient than the previous thermal stress theory in the practical application. 相似文献
139.
C. Busuioc C.D. Ghitulica A. Stoica M. Stroescu G. Voicu V. Ionita L. Averous S.I. Jinga 《Ceramics International》2018,44(8):9433-9441
Bacterial cellulose membranes were employed as templates for calcium phosphates deposition by successive immersion in solutions of Ca(NO3)2·4H2O and (NH4)2HPO4, under ultrasonication. During the wet chemical reaction, mineral phases were loaded on bacterial cellulose fibrils, leading to precursor hybrid composites. These were subjected to a lyophilisation procedure in order to preserve the 3D porous aspect and afterwards to a thermal treatment with the aim of removing the polymeric phase and generating well crystallized structures. Different types of morphologies were achieved by varying the heating rate, as well as the calcination temperature and period. The as-prepared samples and the final ones were investigated from compositional and structural point of view through X-ray diffraction and Fourier-transform infrared spectroscopy and morphologically concerning by scanning electron microscopy. The magnetic properties were also evaluated in order to demonstrate the suitability of the obtained materials for the development of magnetic scaffolds dedicated to hard tissue applications. 相似文献