交联透明质酸的降解及其释药性能

用己二酸二酰肼（ADH）对透明质酸（HA）进行化学修饰，制备交联透明质酸（HA-ADH）薄膜。粘度法测试表明HA-ADH是一种可降解的生物材料，并且与HA相比，HA．ADH的降解速率减慢。在此基础上，研究了以疏水性的替硝唑（TDZ）和亲水性的头孢唑啉钠（CEZ）为模拟药物的HA-ADH药物载体薄膜的释药性能。紫外-可见（UV-Vis）吸收光谱检测表明，HA-ADH是一种疏水性药物TDZ的优良缓释制荆，这是由于TDZ的疏水性和HA-ADH薄膜的缓慢溶胀和降解性能的结合而得到的，药物的释放主要受扩散机制控制。     

基于多随机变量风险约束的水电站长期优化调度

针对市场环境下水电站如何综合考虑电价和来水等不确定性因素下的优化调度是当前研究的热点和难点,建立了考虑电价和来水等不确定性因素的风险约束水电站长期优化调度模型。根据此模型发电商可方便地在预期的目标收益和风险之间进行权衡,达到以较小的风险获得较大的收益的目的。以二滩电厂实算为例,表明了该模型的有效性。     

基于随机分析和概率预测的固定式海洋平台结构风险评估研究

对极端海况进行了环境荷载的联合概率分析。引入了整体不确定性和敏感性分析理论,利用非正态过程、具有不同相关性的多变量随机模拟技术,求解结构的失效概率。提出结构失效分析的新方法,并利用DNV提供的历史数据,对结构失效的后果做了适当分析。以埕北12-C井组平台为实例,对平台甲板高程(Air Gap)失效分析进行了实例计算。结果表明,使用多维联合极值分布理论及相应的求解方法,是离岸工程结构物风险评估的重要手段。     

碳酸盐岩气藏加砂压裂重复改造试验效果分析

针对碳酸盐岩气藏部分气井生产效能低的状况，决定在长庆气田下古生界尝试开展加砂压裂重复改造试验，以期提高低产、低效气井的产量，延长气田稳产期。通过潜心研究、精细设计及现场质检监控，优选了4口老井进行加砂压裂重复改造现场试验。从试验结果来看，虽然只有1口井取得明显的改造效果，但4口井加砂压裂施工都是成功的，说明对碳酸盐岩气藏实施加砂压裂是可以实现的，但在选井思路和压裂工艺方面还有待探索和研究。     

一种考虑分布电容的模糊故障测距算法

提出了一种考虑分布电容的故障测距新算法。该算法基于微分方程描述的线路数学模型，利用单端信息对高压输电线路进行故障测距，保留了解微分方程算法的简单可靠、现实可行、不必考虑衰减直流分量和谐波及电网频率波动影响的特点，同时考虑过渡电阻和分布电容的影响，克服了传统解微分方程法中在经高阻接地故障时测距误差过大和忽略分布电容引起的故障定位不准确的缺点。为了检验算法的精度，进行了大量的动模实验。结果表明，算法原理正确并具有较高的测距精度。     

PURIFICATION AND PROPERTIES OF TRANSGLUTAMINASE FROM STREPTOVERTICILLIUM MOBARAENSE

Transglutaminase (TGase) was separated from the culture broth of an isolated strain of Streptoverticillium mobaraense. The crude enzyme was prepared by centrifugation, ultrafiltration, precipitation by alcohol, centrifugation and freeze‐drying. The yield after these processes was 65–70%. Then the enzyme was purified to homogeneity by chromatography on CM‐cellulose and Sephadex G‐75 on which the yields were about 70% and 80%, respectively; the purified folds reached 2.5–4.7 and 1.08–2.06, respectively. The molecular weight of this TGase was 39,500–40,100 Da by gel filtration chromatography. Optimum enzyme activity was observed in the pH range of 5.0–7.0, and it was maintained stable at 20–40C. The optimal temperature and pH was 52C and 6.0, respectively. At 1 mM and 5 mM metal ion or inhibitors concentration, TGase activity was strongly inhibited by Zn²⁺ and NEM, and not affected obviously by Ba²⁺, Ca²⁺, Co²⁺, Cu²⁺, Fe²⁺, Fe³⁺, Mg²⁺, Mn²⁺, Na⁺ as well as PMSF and EDTA. The effects of these additions on this TGase were compared with those of other microbial TGases.     

储层压力与天然气分布关系——以鄂北塔巴庙地区上古生界储层为例

通过分析塔巴庙地区储层压力的分布特征，建立了研究区泥岩压实曲线，并对影响和控制该区储层压力的主要因素和机理进行了探讨，对“欠压实”剩余压力、生气增压、抬升引起的降压进行了定量计算。分析结果表明，晚三叠-早白垩世末“欠压实”作用和晚侏罗-早白垩世末烃源岩大量生烃作用使塔巴庙地区上古生界形成异常高压；晚白垩系构造抬升降压，使得现今储层为正常-低异常压力。同时证实了“欠压实”对天然气有压力封盖作用，天然气都分布在最大“欠压实”幅度以下的储层当中。烃源岩生气高峰为晚侏罗-早白垩世末，但天然气大规模运移是在晚白垩世，方向以沿过剩压力梯度降低较快的方向（太原-山西组至下石盒子组）垂向运移，通道主要为裂缝，运移的动力主要为异常高压。由于储层致密，聚集成藏能量有限，造成天然气充注不足、气水分异不好、含气饱和度低。     

M型四能级原子与三模场相互作用系统的动力学特性

采用全量子理论,研究了M型四能级原子与三模场相互作用系统的动力学特性,通过数值计算,分析了光场失谐量、初始光子数和原子初始状态对原子布居概率和光子统计演化规律的影响.     

Time Characterization of High Density Gas Jet from a Pulsed Supersonic Nozzle via Laser Produced Plasma

A high-density gas jet supersonic nozzle is reported in this paper. The jitter and actuation time of the nozzle is determined by the pin discharge and laser spark radiation respectively. The jitter time of the nozzle is within 10μs with the backing pressure as high as 25 bar. With a nanosecond laser pulse focused on the gas jet about 1 mm below the nozzle, the actuation time is calculated to be about 15 ms by detecting the laser produced spark radiation, which reveals the existence of the gas jet and the relative gas density evolving with time. Consequently the gas density is estimated to be well above 10^19 cm^-3, compared with theoretical simulations from the nozzle parameters.