Fluorescent chemosensor based on the conjugated polymer incorporating 2,2′‐bipyridyl moiety for transition metal ions

A fluorescent conjugated polymer was synthesized by the polymerization of 1,4‐dibromo‐2,3‐bisbutoxynaphthalene ( M‐2 ) with 5,5′‐divinyl‐2,2′‐bipyridine ( M‐3 ) via Heck reaction. The conjugated polymer shows strong blue–green fluorescence because of the extended π‐electronic structure between the repeating unit 2,3‐bisbutoxynaphthyl group and the conjugated linker 2,2′‐bipyridyl (bpy = 2,2′‐bipyridine) moiety via vinylene bridge. The responsive properties of the conjugated polymer on transition metal ions were investigated by fluorescent and UV–vis spectra. The results show that Cu²⁺ and Ni²⁺ can form nonradiative metal‐to‐ligand charge‐transfer complexes with the polymer, whereas, Zn²⁺ and Cd²⁺ do not produce the pronounced differences from the polymer fluorescence and UV–vis spectra. The fluorescent quenching can probably be attributed to the intramolecular photoinduced electron transfer (PET) or photoinduced charge transfer (PCT). The results can also suggest that 2,2′‐bipyridyl moiety in the main chain backbone of the conjugated polymer can act as the recognition site of a special fluorescent chemosensor for sensitive detection of transition metal ions. © 2008 Wiley Periodicals, Inc. J Appl Polym Sci, 2009     

ATS48软起动器在空调变流量系统中的应用

介绍了商业建筑节能降耗中空调水系统采用变流量方式的必要性及实现途径.通过理论分析,结合实际工程案例,说明了实现变流量的最佳途径是采用软起动器.同时,对软起动器的3种技术原理作了分析比较,对转矩控制型软起动器的典型产品ATS48的主要功能作了说明.     

Simultaneous expansion and harvest of hematopoietic stem cells and mesenchymal stem cells derived from umbilical cord blood

The simultaneous expansion and harvest of hematopoietic stem cells and mesenchymal stem cells derived from umbilical cord blood were carried out using bioreactors. The co-culture of umbilical cord blood (UCB)-derived hematopoietic stem cells (HSCs) and mesenchymal stem cells (MSCs) was performed within spinner flasks and a rotating wall vessel (RWV) bioreactor using glass-coated styrene copolymer (GCSC) microcarriers. The medium used was composed of serum-free IMDM containing a cocktail of SCF 15 ng·mL^?1, FL 5 ng·mL^?1, TPO 6 ng·mL^?1, IL-3 15 ng·mL^?1, G-CSF 1 ng·mL^?1 and GM-CSF 5 ng·mL^?1. Accessory stromal cells derived from normal allogeneic adipose tissue were encapsulated in alginate-chitosan (AC) beads and used as feeding cells. The quality of the harvested UCB-HSCs and MSCs was assessed by immunophenotype analysis, methylcellulose colony and multi-lineage differentiation assays. After 12 days of culture, the fold-expansion of total cell numbers, colony-forming units (CFU-C), CD34⁺/CD45⁺/CD105^? (HSCs) cells and CD34^?/CD45^?/CD105⁺ (MSCs) cells using the RWV bioreactor were (3.7 ± 0.3)- , (5.1 ± 1.2)- , (5.2 ± 0.4)- , and (13.9 ± 1.2)-fold respectively, significantly better than those obtained using spinner flasks. Moreover, UCB-HSCs and UCB-MSCs could be easily separated by gravity sedimentation after the co-culture period as only UCB-MSCs adhered on to the microcarriers. Simultaneously, we found that the fibroblast-like cells growing on the surface of the GCSC microcarriers could be induced and differentiated towards the osteoblastic, chondrocytic and adipocytic lineages. Phenotypically, these cells were very similarly to the MSCs derived from bone marrow positively expressing the MSCs-related markers CD13, CD44, CD73 and CD105, while negatively expressing the HSCs-related markers CD34, CD45 and HLA-DR. It was thus demonstrated that the simultaneous expansion and harvest of UCB-HSCs and UCB-MSCs is possible to be accomplished using a feasible bioreactor culture system such as the RWV bioreactor with the support of GCSC microcarriers.     

葡萄糖氧化酶,颇具前途的溴酸钾替代品

葡萄糖氧化酶具有显著改善面团强度和弹性、提高面包质量的作用。它与其它酶制剂和Vc之间具有协同效应,用于烘焙面包,能获得理想的烘焙效果,同时,葡萄糖氧化酶凭借其天然的优良特性,可作为溴酸钾的一个较为理想的取代物;     

基于神经网络-遗传算法的CT-191合成工艺优化

本文用锌粉还原N-亚硝基二苯胺的产物直接与2-甲基-4（N,N-二苄基）氨基苯甲醛缩合合成了空穴传输材料2-甲基-4（N,N-二苄基）氨基苯甲醛-1,1-二苯腙（CT-191）,采用均匀设计制定试验方案获取原始数据,应用BP人工神经网络对合成过程中工艺参数和一次产品收率的关系建立了模型,并用遗传算法进行优化得到最佳工艺条件：原料2-甲基-4-（N,N-二苄基）氨基苯甲醛：N-亚硝基二苯胺约为1：2．5,还原时间为1h,缩合时间为2h,预测收率为96．28％。验证实验的结果为95．98％．和预测值基本吻合。为化学生产工艺的优化探索了一条新途径。     

一项建材创新技术的价值评估

技术价值评估是市场经济条件下技术应用的前提条件。文中以一项高分子建材生产技术为案例,研究了收益法、成本法、市场法在该成套技术价值评估中的应用,提出了技术价值是一个价值域区间,而不是一个特定值的观点,评估值的一个解对应于一组表征评估目的性的约束条件。     

酚类化合物对Shk1发光细菌毒性的定量结构-活性相关研究

以PM3算法计算所得的量子化学参数为自变量,应用偏最小二乘法(PLS),建立酚类化合物对Shk1发光细菌毒性的定量结构-活性相关(QSAR)模型。模型所提取的PLS主成分所能解释的因变量总方差的比例Q2cum为0．810,表明模型具有较好的稳定性和预测能力。模型结果表明,影响酚类化合物对Shk1发光细菌毒性的主要因素是分子次最高占据轨道能 (EHOMO 1)、相对分子质量(Mr)、平均分子极化率(α)和分子总的核-核排斥能(CCR),酚类化合物对Shk1发光细菌的毒性值随着分子EHOMO 1的增大而减小,随着Mr、α和CCR的增大而增大。     

在空气和真空中退火的316奥氏体不锈钢的力学性能及微观结构分析

对316奥氏体不锈钢在空气和真空条件下进行退火,在恒定的拉伸速度下进行室温拉伸强度试验,并利用扫描电镜和透射电镜观察拉伸试验后试样的表面和近表面的微观结构.退火试样的抗拉强度和应力值高于未退火试样;在真空炉中退火的试样,其抗拉强度及在特定应变量下的应力值均高于在空气炉中退火的试样.在真空炉中退火的316不锈钢的表面氧化层分内、外两层,外层由均匀、致密的亚微细晶粒组成;而在空气炉中生成的氧化层结构复杂,和基体的结合强度非常低.氧化层-基体界面是一个充满活力的位错源,真空炉退火试样的位错密度大于空气炉退火试样.氧化层的微观结构和两种试样位错密度的不同是导致产生不同拉伸性能的重要原因.     

高撕裂阻力金属延性断裂韧度测试方法探讨

讨论现行J积分测试方法在测定高撕裂阻力(dJ/da)金属延性断裂韧度JIC时所遇到的困难,结合工业金属冶金组织特性分析裂纹试样受力后的钝化、启裂及裂纹稳态扩展过程,并给出此类材料的JIC测试方法.研究指出,裂纹启裂过程是一个由塑性变形为主导的裂纹顶端伸张变形和裂尖钝化向微孔型断裂过渡的过程;开裂点实际上是在漫长的裂纹前沿上大部分地方完成这一过渡,裂尖伸张区(stretched zone, SZ)达到饱和,微孔开裂机制开始占据主导地位的标志.采用直线和幂函数形式拟合启裂后的J-Δa关系,以及采用幂函数形式拟合启裂前与启裂后全过程的J-Δa关系均可得到很相近的相关性,且在一定程度上,后者显得更合理.