DNA vaccination is an effective means of eliciting strong antibody responses to a number of viral antigens. However, DNA immunization alone has not generated persistent, high-titer antibody and neutralizing antibody responses to human immunodeficiency virus type 1 (HIV-1) envelope glycoprotein (Env). We have previously reported that DNA-primed anti-Env antibody responses can be augmented by boosting with Env-expressing recombinant vaccinia viruses. We report here that recombinant Env protein provides a more effective boost of DNA-initiated antibody responses. In rabbits primed with Env-expressing plasmids, protein boosting increased titer, persistence, neutralizing activity, and avidity of anti-Env responses. While titers increased rapidly after boosting, avidity and neutralizing activity matured more slowly over a 6-month period following protein boosting. DNA priming and protein immunization with HIV-1 HXB-2 Env elicited neutralizing antibody for T cell line-adapted, but not primary isolate, viruses. The most effective neutralizing antibody responses were observed after priming with plasmids which expressed noninfectious virus-like particles. In contrast to immunizations with HIV-1 Env, DNA immunizations with the influenza virus hemagglutinin glycoprotein did not require a protein boost to achieve high-titer antibody with good avidity and persistence. 相似文献
With the increasing client population and the explosive volume of Internet media content, the peer-to-peer networking technologies and systems provide a rapid and scalable content distribution mechanism in the global networks. The BitTorrent protocol and its derivatives are among the most popular peer-to-peer file sharing applications, which contribute a dominant fraction of today??s Internet traffic. In this paper, we conduct the performance measurement and analysis of BitTorrent systems with an extensive volume of real trace logs. We use several downloading-side metrics, including overall downloading time, maximum of downloading bandwidth, average bandwidth utilization, maximum of downloading connections, and average number of active connections, to derive various interesting results from the downloading-side aspect of network resource usage. Performance examination learns many new observations and characteristics into the virtue of BitTorrent protocols and systems, thereby providing beneficial information for bandwidth allocation and connection control in BitTorrent client applications. Therefore, this study is complementary to many previous research works that mainly focused on system-oriented and uploading-side performance measurements. 相似文献
Knowledge and Information Systems - Question answering over knowledge graph (KGQA), which automatically answers natural language questions by querying the facts in knowledge graph (KG), has drawn... 相似文献
Surrogate models have been widely applied to correlate design variables and performance parameters in turbomachinery optimization applications. With more design variables and uncertain factors taken into account in an optimization design problem, the mathematical relations between the design variables and the performance parameters might present linear, low-order nonlinear or even high-order nonlinear characteristics, and are usually analytically unknown. Therefore, it is required that surrogate models have high adaptability and prediction accuracy for both the linear and nonlinear characteristics. The paper mainly investigates the effectiveness of an adaptive region segmentation combining surrogate model based on support vector regression and kriging model applied to a transonic axial compressor to approximate the complicated relationships between geometrical variables and objective performance outputs with different sampling methods and sizes. The purpose is to explore the prediction accuracy and computational efficiency of this adaptive surrogate model in real turbomachinery applications. Three different sampling techniques are studied: (1) uniform design; (2) Latin hypercube sampling method; (3) Sobol quasi-random design. For the low dimensional case with five variables, the adaptive region segmentation combining surrogate model performs better (not worse) than the single component surrogate in terms of prediction accuracy and computational efficiency. In the meanwhile, it is also noted that the uniform design applied to the adaptive surrogate model has more advantages over the Latin hypercube sampling method especially for the small sample size cases, both performing better than the Sobol quasi-random design. Moreover, a high dimensional case with 12 variables is also utilized to further validate the prediction advantage of the adaptive region segmentation combining surrogate model over the single component surrogate, and the computational results favor it. Overall, the adaptive region segmentation combining surrogate model has produced acceptable to high prediction accuracy in presenting complex relationships between the geometrical variables and the objective performance outputs and performed robustly for a transonic axial compressor problem.
Generative adversarial networks (GANs) are paid more attention to dealing with the end-to-end speech enhancement in recent years. Various GAN-based enhancement methods are presented to improve the quality of reconstructed speech. However, the performance of these GAN-based methods is worse than those of masking-based methods. To tackle this problem, we propose speech enhancement method with a residual dense generative adversarial network (RDGAN) contributing to map the log-power spectrum (LPS) of degraded speech to the clean one. In detail, a residual dense block (RDB) architecture is designed to better estimate the LPS of clean speech, which can extract rich local features of LPS through densely connected convolution layers. Meanwhile, sequential RDB connections are incorporated on various scales of LPS. It significantly increases the feature learning flexibility and robustness in the time-frequency domain. Simulations show that the proposed method achieves attractive speech enhancement performance in various acoustic environments. Specifically, in the untrained acoustic test with limited priors, e.g., unmatched signal-to-noise ratio (SNR) and unmatched noise category, RDGAN can still outperform the existing GAN-based methods and masking-based method in the measures of PESQ and other evaluation indexes. It indicates that our method is more generalized in untrained conditions. 相似文献