Synergistic decrease of clonal proliferation, induction of differentiation, and apoptosis of acute promyelocytic leukemia cells after combined treatment with novel 20-epi vitamin D3 analogs and 9-cis retinoic acid

Patients with acute promyelocytic leukemia (APL) usually relapse after all-trans retinoic acid (RA) treatment because this therapy fails to eradicate the malignant clone. Our data showed that KH 1060 and other 20-epi vitamin D3 analogs alone were potent inhibitors of clonal growth of NB4 cells, an APL cell line (ED50, approximately 5 x 10(-11) M). The combination of KH 1060 and 9-cis-RA synergistically and irreversibly enhanced this effect. Neither KH 1060 nor 9-cis-RA (10(-6) M, 3 d) were strong inducers of differentiation of NB4 cells. However, 98% of the cells underwent differentiation to a mature phenotype with features of both granulocytes and monocytes after exposure to a combination of both compounds. Apoptosis only increased after incubation of NB4 cells with 9-cis-RA alone (28%) or with a combination of 9-cis-RA plus KH1060 (32%). Immunohistochemistry showed that the bcl-2 protein decreased from nearly 100% of the wild-type NB4 cells to 2% after incubation with a combination of KH 1060 and 9-cis-RA, and the bax protein increased from 50% of wild-type NB4 cells to 92% after culture with both analogs (5 x 10(-7) M, 3 d). Western blot analysis paralleled these results. Studies of APL cells from one untreated individual paralleled our results with NB4 cells. Taken together, the data demonstrated that nearly all of the NB4 cells can be irreversibly induced to differentiate terminally when exposed to the combination of KH 1060 and 9-cis-RA.     

The effect of a two-week anti-orthostatic hypokinesia on the cardiac function and on the latter''s regulation in monkeys

Medical practice guidelines are being developed rapidly and advocated as a crucial component in transforming and improving the delivery of health care services. However, little is known about the ability of hospitals to institute guidelines outside of experimental settings. This article approaches guideline implementation from the viewpoint of process innovation assimilation and presents specific recommendations for organizational tactics leading to successful implementation of practice guidelines.     

基于激光多普勒测速法的2×106m/s2高冲击校准技术

在高冲击传感器动态性能校准中,实现高量程、宽脉冲激励是目前高冲击校准急需解决的问题。采用速度改变法校准原理,以气炮为激励源,利用激光多普勒测速方法测量砧体运动速度,建立高冲击校准系统。分析碰撞系统的作用原理,提出调整和控制激励脉宽的方法;分析测速误差,提出双光束差动侧向测速方法。实验结果表明:高冲击校准系统校准量程为2×106m/s2,激励脉宽在2×106m/s2时大于100μs,校准冲击灵敏度的扩展不确定度小于5%(k=2)。     

BP网络模型对人脸朝向的识别

研究BP神经网络模型,通过计算机模拟人脑建立神经元网络,使用一部分人脸朝向信息作为训练的实例集,训练稳健后,可以推广应用判断其他人脸的朝向信息,以实现计算机自动识别人脸朝向。先对图片进行归一化等预处理,再应用主成分分析提取特征信息,每幅提取出的特征信息都是8个数据的列向量,构建一个8个输入、17个隐含、3个输出的三层BP网络模型。将训练实例集的特征向量代入训练,调整参数后保证其性能和收敛速度。最后通过大量实验验证,计算机识别的误判率仅为6.7%,模型可靠。     

一种基于光照变化补偿的颜色识别方法

针对RoboCup中型组足球机器人比赛中光照变化会使颜色发生色彩漂移,影响颜色识别的准确性问题,提出一种基于光照变化补偿的颜色识别方法。该方法首先利用球的历史信息预测球的位置,然后在较小的区域内用基于色调直方图反向投影的方法找到球后,通过球的亮度直方图的变化计算出光照变化率,用于动态补偿颜色查找表。实验表明该方法能提高颜色识别的光照自适应性。     

白河、潮河底栖动物分布与特征研究

在对潮河和白河北京境内段底栖动物调查基础上,采用群落分析法并运用BI指数和Shannon-Wiener多样性指数进行水质评价.结果表明:本次共采集底栖动物44种,水生昆虫及幼虫占比最大.底栖动物种类丰富度白河优于潮河.河流评价结果显示,潮河和白河水质整体为优.BI指数法评价中,潮河和白河以清洁为主.Shannon-Wiener多样性指数评价结果中,白河以轻污染为主,潮河以中污染为主.BI指数法适用性较好,而且评价结果较为准确,评价结果对潮河、白河水质判别具有借鉴作用.     

人工神经网络技术在电工产品可靠性实验中的应用(理论部分)

本文简要介绍了人工神经网络，BP神经网络的结构、训练和学习规则以及人工神经网络非线性时间序列预测的应用概况。