Optimisation of the medium composition for production of protease and soybean peptides by Bacillus subtilis SHZ using response surface methodology

Responses surface methodology was employed to enhance the production of protease and soybean peptides by Bacillus subtilis SHZ. For screening of medium composition significantly influencing protease and soybean peptides yield, the two-level Plackett–Burman design was used. Among thirteen variables tested; KH₂PO₄, glucose and defatted soybean flour (DSF) were selected based on their high significant effect on both protease activity and soybean peptides yield. Then, a three-level Box–Behnken design was employed to optimise the medium composition for the production of the protease and soybean peptides in submerged fermentation. Mathematical models were then developed to show the effect of each medium composition and their interactions on the production of protease and soybean peptides. The model estimated that, the maximal protease activity (320 ± 1 U mL⁻¹) could be obtained when the concentrations of glucose, KH₂PO₄, DSF were set at 8–9 g L⁻¹, 2–3 g L⁻¹, 55–65 g L⁻¹, respectively; while a maximal yield of soybean peptides (8.5 ± 0.1 g L⁻¹) could be achieved when the concentrations of glucose, KH₂PO₄, DSF were set at 7–9 g L⁻¹, 3–4 g L⁻¹ and 55–58 g L⁻¹, respectively. These predicted values were also verified by validation experiments.     

Tb doped CoNbZr soft magnetic films with high anisotropy field for applications in GHz frequency region

CoNbZr films for an adjustable magnetic anisotropy field H_k by doping with rare-earth of different atomic ratio Tb element were obtained in this work. The effect of Tb addition ranged from 0 to 4 at.% on the magnetic properties of the amorphous CoNbZr films was further investigated. The results show that the magnetic anisotropy field H_k increases sharply with the addition of Tb while the coercivity H_ch along the hard axis and H_ce along the easy axis change slightly with increasing Tb content in the films. As a consequence, CoNbZr film doped with 2 at.% of Tb exhibits excellent soft magnetic properties with a saturation magnetization 4πM_s of 8.9 kG, a hard axis coercivity H_ch of 1.79 Oe, a easy axis coercivity H_ce of 1.4 Oe and a magnetic anisotropy field H_k of 87 Oe. The measured ferromagnetic resonance frequency f_FMR of this film is 2.30 GHz. The real permeability μ′ is about 100, which is maintained up to 2 GHz. In addition, there is a broad band of the imaginary permeability μ″ over a large frequency band, indicating high losses. Therefore, Tb doped CoNbZr film is an excellent candidate for high frequency applications such as electromagnetic interference suppressors.     

一类稀疏效应下捕食模型正平衡态解的稳定性

考虑了一类齐次Neumann边界条件下具稀疏效应和扩散的捕食者-食饵模型，通过建立适当的Lyapunov函数得到其唯一的正平衡态解的全局渐近稳定性．     

Heat flux profile of the furnace wall of a 300 MWe CFB boiler

It is important to properly understand and calculate the distributions of the heat flux and the heat transfer coefficient in a furnace for designing a circulating fluidized bed (CFB) boiler, especially for one with supercritical parameters. An experimental study of the heat transfer in a commercial 300 MWe CFB boiler was conducted in this work. The heat flux from the bed to the waterwall was measured by a heat flux meter at four different heights. The influence of suspension density and bed temperature was analyzed by measuring the pressure profile and temperature profile in the furnace. In addition a numerical modeling study on the two phase flow in the furnace was carried out. The results indicate that the density of the solids suspension was non-uniform in the cross section at a certain height, and decreased gradually with increasing furnace height. Consequently, the distribution of heat flux in the horizontal plane was non-uniform and decreased along the height of the furnace. The heat flux showed a decreasing trend near the center.     

First successful fabrication of high-performance all-refractory-metal (Ta-Au) GaAs FET using very highly doped N+-layers and nonalloyed ohmic contacts

An all-refractory-metal GaAs MESFET (ARFET) making use of a Ta Schottky barrier with a thick gold overlayer for the source, gate and drain, and very highly doped N+-layers (2x1019 cm-3) to achieve low-resistivity nonalloyed ohmic contacts, has been successfully fabricated. These have a 400 ?m gate periphery and 0.6 ?m gate length and measured an associated gain of 10.22 dB and a noise figure of 2.14 dB at 8 GHz. The ARFETs were fabricated on epitaxial layers grown by MBE. Only one mask was used to simultaneously define source, gate and drain regions via a plasma dry-etch technique.     

用于煤浆系统研究的光子输运程序及其实验标定

本文介绍了为进行煤浆系统光子输运研究而专门设计的Ｍｏｎｔｅ Ｃａｒｌｏ程序Ｍｃｐｈｏｔ，采用实验方法对该程序进行了标定，实验结果和计算结果符合得很好。     

面向目标控制照明的合成波源偏移

应用Fourier有限差分算子，研究目标控制照明的合成波源偏移成像。平面波源与小束波源这些合成波源在目标控制照明下的偏移，都是通过增强对目标区域的有效照明来改善成像质量，通过减少非目标区域的计算冗余来提高计算效率。由分析和应用合成平面波源偏移和控制照明技术扩展开去，综合考虑平面波源和小束波源的合成与偏移，并通过有效照射合成波源的概念，分析合成波源的有利照明，进行面向目标区域的控制照明和合成波源偏移。最后，对具有速度强横向变化和陡倾角反射的复杂2D盐丘模型数据，做了相应的合成波源偏移数值试验。     

Lipase-catalysed acidolysis of lard with caprylic acid to produce structured lipid

Enzymatic acidolysis of lard with caprylic acid was investigated. Of the five lipases that were tested in the initial screening, immobilised lipase TL IM from Thermomyces lanuginosus resulted in the highest incorporation of caprylic acid into lard. This enzyme was further studied for the effect of enzyme load, organic solvent, substrate ratio, reaction time and temperature. HPLC was used to analyse the products from the acidolysis reaction. The highest incorporation was attained at 15% enzyme load. Among the solvents tested, n‐hexane was the best reaction medium for the acidolysis of lard with caprylic acid. Time course studied suggests that the incorporation of caprylic acid into lard was increased up to 37.7 mol% after 24 h. Desirable mole ratio of lard to caprylic acid was 1:2, caprylic acid incorporation up to 34.2 mol%. Temperature had no significant effect on enzyme activity in the range of 40–80 °C.     

机械CAD软件系统面向对象建模方法研究

以面向对象的减速器ＣＡＤ系统的分析建模为例，对机械ＣＡＤ系统采用面得对象技术建模的方法进行了研究，总结出此类ＣＡＤ系统面向对象建模的一般特点。