Improved method for HgI2 crystal growth and detector fabrication

Instead of the temperature oscillation method (TOM), a modified vapor growth method was applied in this paper to grow large HgI₂ crystals with fewer lattice defects by providing a relatively stable temperature field during growth. And a new processing technique of HgI₂ crystals was developed for detector fabrication by merging solution string-sawing and hand-cleaving. The lattice deformations caused during cleaving were reduced greatly by eliminating passivated layers on the sawed crystal platelets before cleaving.     

On the Realization of Current－Mode Continuous－Time Operational Transconductance Capacitance Filter

ＯｎｔｈｅＲｅａｌｉｚａｔｉｏｎｏｆＣｕｒｒｅｎｔ－ＭｏｄｅＣｏｎｔｉｎｕｏｕｓＴｉｍｅＯｐｅｒａｔｉｏｎａｌＴｒａｎｓｃｏｎｄｕｃｔａｎｃｅＣａｐａｃｉｔａｎｃｅＦｉｌｔｅｒ￥ＧｕｏＪｉｎｇｂｏａｎｄＨａｎＱｉｎｇｑｕａｎ（ＣｈａｎｇｃｈｕｎＰｏｓ...     

扑炎痛的合成新法

以水杨酸和对硝基苯酚为原料,在三氯氧磷存在下进行酯化,然后催化加氢还原硝基,最后用乙酐进行二酰化制得扑炎痛,三步收率分别为80.8％、90.4％和88.5％。     

Residues Val254, His256, and Phe259 of the angiotensin II AT1 receptor are not involved in ligand binding but participate in signal transduction

The role of the external third of helix VI of the angiotensin II (AII) AT1 receptor for the interaction with its ligand and for the subsequent signal transduction was investigated by individually replacing residues 252-256 by Ala, and residues 259 or 261 by Tyr, and permanently transfecting the resulting mutants to Chinese hamster ovary (CHO) cells. Binding experiments showed no great changes in affinity of any of the mutants for AII, [Sar1]-AII, or [Sar1, Leu8]-AII, but the affinity for the nonpeptide antagonist DuP753 was significantly decreased. The inositol phosphate response to AII was remarkably decreased in mutants V254A, H256A, and F259Y. These results indicate that AT1 residues Val254, His256, and Phe259 are not involved in ligand binding but participate in signal transduction. Based in these results and in others from the literature, it is suggested that, in addition to the His256 imidazole ring, the Phe259 aromatic ring interacts with the AII's Phe8, thus contributing to the signal-triggering mechanism.     

Selective monitoring of peptidase activities with synthetic polypeptide substrates and polyion-sensitive membrane electrode detection

A novel method to monitor specific peptidase activities in biological samples as complex as undiluted plasma/blood is described. The approach is based on the design of synthetic polypeptide substrates in which di- or triarginine sequences are linked to each other via one or more other amino acids recognized specifically by the peptidase to be determined. Detection of chymotrypsin and renin activities using synthetic substrates P4 (F-R-R-R-F-V-R-R-F-NH2) and P5 (R-R-R-L-L-R-R-L-L-R-R-R), respectively, serves to demonstrate the principles of this new assay system. A polyion-sensitive membrane electrode, prepared by doping polymer films with dinonylnaphthalene-sulfonate (DNNS), is shown to exhibit significant nonequilibrium electromotive force (EMF) responses toward these and other polycationic substrates at microgram/milliliter levels under physiological conditions. The same electrode, however, exhibits much smaller total EMF response toward the shorter fragments of the synthetic peptides generated by peptidase activity; hence, the addition of peptidase to a solution containing the synthetic substrate yields a change in electrode EMF response, the rate of which is proportional to the activity of peptidase present. Other synthetic polycationic peptides as well as natural polycationic peptides (e.g., protamine) that lack specific cleavage sites for chymotrypsin and renin, yet are detected by the DNNS-based membrane electrode, do not elicit any significant change in EMF response in the presence of the peptidases, confirming the feasibility and utility of the proposed bioanalytical method.     

低能重离子及其碰撞产生的级联原子在生物材料中的直接作用范围

用Ｍｏｎｔｅ Ｃａｒｌｏ法模拟计算了３０ｋｅＶ和２００ｋｅＶ的Ｎ＾＋与１１０ｋｅＶ的Ｆｅ＾＋在模拟细胞中的射程分布和径迹结构，并将１１０ｋｅＶＦｅ＾＋模拟计算的结果与ＲＳＢ测量的结果相比较，发现计算与测量的结果吻合较好，离子的作用范围小于１μｍ。计算和实验的结果都说明能量相当低的重离子不大可能直接作用引起麦胚深层生物效应。     

用复合烷基锂引发丁二烯聚合

对以环己烷为溶剂、四氢呋喃为微观结构调节剂、复合烷基锂为引发剂的丁二烯聚合反应过程进行了研究，考察了不同引发温度、不同配比的复合烷基锂对聚合反应的影响，并与以正丁基锂为引发剂的丁二烯聚合反应过程进行了比较。     

Initial results of an in vitro fertilization and embryo transfer program in a private health institution

Between October 1994 and the end of February 1995 115 patients were included in our programme. In these patients we monitored 141 cycles, performed 128 punctures and 100 ET. In 14 instances (9.2%) the cycle was abolished. By puncture of 1468 follicles 1370 oocytes were obtained which corresponded to a recovery rate of 93.3%. On average by aspiration 11.9 oocytes per patient were obtained. From the total number of acquired oocytes 54.6% were fertilized and 43.5% divided. Of 596 embryos 290 were transferred and the remainder was vitrified. A total of 17 clinical pregnancies were achieved, incl. 5 which terminated by abortion, the remainder proceeds. The authors are aware that they present a relatively small group during a short period of time, and thus the first results of the centre cannot be presented as a current standard. Despite this the results permit some optimism for the future and indicate a certain amount of standardization of the authors' clinical and laboratory work.     

Rat and human pancreatic islet cells contain a calcium ion independent phospholipase A2 activity selective for hydrolysis of arachidonate which is stimulated by adenosine triphosphate and is specifically localized to islet beta-cells

The recent demonstration that myocardial Ca(2+)-independent phospholipase A2 exists as a complex of catalytic and regulatory polypeptides that is modulated by ATP has suggested a novel mechanisms through which alterations in glycolytic flux can be coupled to the generation of eicosanoids which facilitate insulin secretion. To determine the potential relevance of this mechanism, we examined the kinetic characteristics, substrate specificities, and cellular locus of phospholipase A2 activity in pancreatic islets. Rat pancreatic islets contain a Ca(2+)-independent phospholipase A2 activity which is optimal at physiologic pH, preferentially hydrolyzes phospholipid substrates containing a vinyl ether linkage at the sn-1 position, and prefers arachidonic acid compared to oleic acid in the sn-2 position. Rat islet Ca(2+)-independent phospholipase A2 activity is inhibited by the mechanism-based inhibitor (E)-6-(bromomethylene)-3-(1-naphthalenyl)-2H-tetrahydropyran-2-one and is stimulated by ATP. Purification of beta-cells from dispersed pancreatic islet cells by fluorescence-activated cell sorting demonstrated that beta-cells (but not non-beta-cells) contain Ca(2+)-independent, ATP-stimulated phospholipase A2 activity. Remarkably, clonal RIN-m5f insulinoma cells, which possess a defect in glucose-induced insulin secretion, contain a Ca(2+)-independent phospholipase A2 which is not modulated by alterations in ATP concentration. Collectively, these results and those of an accompanying paper [Ramanadham et al. (1993) Biochemistry (following paper in this issue)] implicate Ca(2+)-independent phospholipase A2 as a putative glucose sensor which can couple alterations in glycolytic metabolism to the generation of biologically active eicosanoids and thereby facilitate glucose-induced insulin secretion.