全站仪三点前方交会测量风电塔架倾斜

倾斜测量是了解高耸构筑物结构安全的一项重要工作,以风电塔架的倾斜观测为例,采用三点前方交会观测塔架倾斜,对交会点的点位精度进行分析,并将塔架倾斜数据与基础倾斜数据相比对.结果表明,三点前方交会法的倾斜数据可靠、测量精度高,能够为高耸构筑物的结构安全提供有力保障.     

Tetrandrine Inhibits Skeletal Muscle Differentiation by Blocking Autophagic Flux

Tetrandrine is well known to act as a calcium channel blocker. It is a potential candidate for a tumor chemotherapy drug without toxicity. Tetrandrine inhibits cancer cell proliferation and induces cell death through apoptosis and autophagy. As cancer patients usually experience complications with sarcopenia or muscle injury, we thus assessed the effects of tetrandrine on skeletal muscle cells. We report in this study that a low dose of tetrandrine (less than 5 μM) does not affect the proliferation of C2C12 myoblasts, but significantly inhibits myogenic differentiation. Consistently, tetrandrine inhibited muscle regeneration after BaCl₂-induced injury. Mechanistic experiments showed that tetrandrine decreased the p-mTOR level and increased the levels of LC3 and SQSTM1/p62 during differentiation. Ad-mRFP-GFP-LC3B transfection experiments revealed that the lysosomal quenching of GFP signals was suppressed by tetrandrine. Furthermore, the levels of DNM1L/Drp1, PPARGA1 and cytochrome C (Cyto C), as well as caspase 3 activation and ROS production, were decreased following tetrandrine administration, indicating that the mitochondrial network signaling was inhibited. Our results indicate that tetrandrine has dual effects on autophagic flux in myoblasts during differentiation, activation in the early stage and blockade in the late stage. The ultimate blocking of autophagic flux by tetrandrine led to the disruption of mitochondria remodeling and inhibition of myogenic differentiation. The inhibitory effects of tetrandrine on skeletal muscle differentiation may limit its application in advanced cancer patients. Thus, great attention should be paid to the clinical use of tetrandrine for cancer therapy.     

Identification and Functional Analysis of the Caffeic Acid O-Methyltransferase (COMT) Gene Family in Rice (Oryza sativa L.)

Caffeic acid O-methyltransferase (COMT) is one of the core enzymes involved in lignin synthesis. However, there is no systematic study on the rice COMT gene family. We identified 33 COMT genes containing the methyltransferase-2 domain in the rice genome using bioinformatic methods and divided them into Group I (a and b) and Group II. Motifs, conserved domains, gene structure and SNPs density are related to the classification of OsCOMTs. The tandem phenomenon plays a key role in the expansion of OsCOMTs. The expression levels of fourteen and thirteen OsCOMTs increased or decreased under salt stress and drought stress, respectively. OsCOMTs showed higher expression levels in the stem. The lignin content of rice was measured in five stages; combined with the expression analysis of OsCOMTs and multiple sequence alignment, we found that OsCOMT8, OsCOMT9 and OsCOMT15 play a key role in the synthesis of lignin. Targeted miRNAs and gene ontology annotation revealed that OsCOMTs were involved in abiotic stress responses. Our study contributes to the analysis of the biological function of OsCOMTs, which may provide information for future rice breeding and editing of the rice genome.     

用不对称醚作调节剂合成苯乙烯-异戊二烯-丁二烯橡胶的反应动力学

以苯乙烯(St)、异戊二烯(Ip)和丁二烯(Bd)为单体,正丁基锂(n-BuLi)为引发荆,乙基乙二醇叔丁基醚(BET)为结构调节剂,合成了线型无规结构的苯乙烯-异戊二烯-丁二烯橡胶(SIBR),进行了聚合反应动力学研究,并与以四氢呋喃(THF)为结构调节剂的体系进行了比较.结果表明,BET的加入提高了聚合反应速率;随反应温度的升高和BET/n-BuLi(摩尔比)的增大,聚合反应速率加快,尤其是St的反应速率提高显著;BET调节聚合速率的能力明显高于THF.     

含短纤维胎面胶的混炼工艺

介绍了含短纤维胎面胶的混炼工艺,分析了在混炼过程中短纤维在橡胶中的混合分散模型.指出当短纤维-橡胶复合材料处于最佳黏度状态时,混炼效果最佳.     

船用热轧球扁钢的研制与开发

安钢根据市场需求,通过合理的工艺设计及严密的生产组织,研制开发出船用热轧球扁钢。产品力学性能、几何尺寸和表面质量等完全达到国标GB9945—2001及ABS、CCS、BV、LR、GL等五国船规技术要求。     

涡轮流量计故障分析与处理

在科威特27#集油站投产保运期间,外输轻质油流量计投用时发现差压流量计指示正常,而FC50系列涡轮流量计无指示,轻质油外输工艺流程如图1。FC50系列涡轮流量计为轻质油外输累积计量流量计,业主十分关注,需尽快解决。经自己认真检查、分析、处理,使问题得到了圆满解决,为公司赢得了荣誉。