Protoplast from <Emphasis Type="Italic">β</Emphasis>-carotene-producing fungus <Emphasis Type="Italic">Blakeslea trispora</Emphasis>: Preparation,regeneration and validation

The effects of key parameters on the preparation and regeneration of protoplast from the β-carotene-producing fungus Blakeslea trispora were discussed in this paper, including the combination of various enzymes, mycelial age, digesting time and temperature, pH value, osmotic stabilizers, pretreatment, culture medium and culture method. Under the condition of mixed enzymes in osmotic stabilizer (0.6 M NaCl) combined with 2% lysozyme, 3% cellulase and 3% snailase, the highest protoplast yield, as high as 7.48×106 protoplasts/mL, was obtained when mycelial age was 60 h at pH 5.0–6.0 with digesting for 14–16 h at 28 °C. After purification of the obtained protoplasts, they were regenerated in PDA regenerative medium using bilayer plate culture method. To validate the usability of the protoplasts, a novel plasmid with green fluorescent protein (GFP) was used in transformation for easy visual observation. The results showed that the protoplasts prepared by the optimized method were active and applicable in further gene manipulation experiments. This work was presented at 13 ^th YABEC symposium held at Seoul, Korea, October 20–22, 2007.     

方圆坯铸机开发新断面自动化程序的实现

文章介绍了包钢连铸机结晶器液位、喷淋水、热坯压力等主要参数实现自动控制的过程,阐述了上述参数如何根据不同铸坯断面而改变,并针对钢厂开发新断面如何实现参数的切换提出解决办法.     

基于显微视觉的宏/微双重驱动微动台的自动标定

提出了基于压电技术的微操作系统的自动标定方法,采用混合式步进电机直接驱动的宏动平台,实现系统大行程宏动定位,安装在宏动平台上的压电陶瓷驱动的微动平台和精密光栅,实现亚微米级的分辨率和定位精度,通过以上两部分实现定位机构的全闭环反馈控制,采用显微视觉反馈获取微动台操作器在图像中的位置信息进行标定。实验结果表明:系统的动态和稳定性能良好,自动标定运算速度快,运行速度达到11 frame/s,实现了对系统的精确标定,标定精度达到0.1μm。     

论数码艺术的发展趋势研究

数码艺术在当代的发展中具有明显特点,是对传统艺术的继承与超越,为艺术家们以及设计师们提供了全新的创作视角,使艺术走上了新的发展空间.数码艺术在当代的发展趋势体现了数码艺术与技术的分工,实现商业性与艺术性的结合.     

全球粮食危机中化肥产业面临的问题与对策

从国内外整体形势来看,通货膨胀带来的粮食危机正在全球蔓延,化肥供应不稳、价格不断增长已经对粮食生产以及农业甚至国家经济平稳发展形成了严重影响.能源和粮食紧张将是我国长期面临的问题,这就决定了化肥产业的发展方向.在产业内部,必须走向大型化基础产业与小型服务型产业结合的道路,各种产品应该根据商品特性和农业需求进行市场细分.建议国家加强对化肥产业的宏观调控、转变调控方式,加强市场需求的预警预测和实时调节,提高国家对肥料施用技术的支持力度.同时建议企业做好信息分析工作,及时做出有利于国家和企业长远发展的决策,不应盲目跟随涨价而丧失市场,当前应利用化肥涨价的时机开展技术服务工作,抢占化肥市场制高点.     

Synthesis of TiO<Subscript>2</Subscript> Hybrid Molecular Imprinted Polymer for Ethofumesate Linked by Silane Coupling Agent

TiO₂ hybrid molecular imprinted polymer (MIP) for ethofumesate using methacrylic acid (MAA) as the functional monomer and silane coupling agent 3-(trimethoxysilyl) propylmethacrylate (KH570) as organic–inorganic connective bridge was synthesized via photo-excitation method. Hydrogen bond was proved to act between MAA and ethofumesate for pre- and post-polymerization binding properties as testified by UV spectrometric method. KH570 modified TiO₂ nanoparticles were prepared via sonochemical reaction, which can accelerate hydrolysis, increase collision chance for the reactive system and improve the dispersion of the nanoparticles. Scanning electron microscope (SEM), transmission electron microscope (TEM), binding and the adsorption kinetics experiments as well as thermogravimetric analysis (TGA) were employed for characterization. The results indicated that the hybrid MIP revealed a larger surface area and more ordered imprinting cavities with improved thermal stability compared to organic-only MIP. Furthermore, faster adsorption kinetics and enhancive adsorption capacity were achieved, which made it promising in chemical sensor applications.