有机溶剂中树脂催化丙酮氰醇分解反应的动力学模型

Decomposition of acetone cyanohydrin is the first-step reaction for preparing (S)-α-cyano-3-phenoxybenzyl alcohol (CPBA) by the one-pot method in organic media. Considering the compatibility of bio-catalysts with chemical catalysts and the successive operation in the bioreactor, anion exchange resin (D301) was used as catalyst for this reaction. External diffusion limitation was excluded by raising rotational speed to higher than 190r·min-1 in both solvents. Internal diffusion limitation was verified to be insignificant in this reaction system. The effect of acetone cyanohydrin concentration on the reaction was also investigated. An intrinsic kinetic model was proposed when the mass transfer limitation was excluded, and the average deviation of the model is 10.5%.     

酿酒酵母生物转化蛋氨酸生产S-腺苷-L-蛋氨酸

摇瓶考察筛选酿酒酵母（zjus1)培养1d后补加蛋氨酸和葡萄糖生产S－腺苷－L－蛋氨酸（SAM），并考察了酵母提取物，补加蛋氨酸浓度，葡萄糖浓度及转化pH对酵母细胞的生长和SAM产量的影响，15LB.Braun罐间歇培养及转化过程实验表明，溶氧提高利于碳源利用、细胞生长和SAM产量提高，SAM水平可达800mg/L，酵母密度DCW=15g/L，蛋氨酸转化率约30％。