Interaction of bacteriophage R17 and reovirus type III with the clay mineral allophane

A study has been made of the adsorption of bacteriophage R17 and reovirus type 3 by the amorphous aluminosilicate clay mineral allophane. In agreement with previous studies of virus adsorption to other minerals such as montmorillonite and aluminium hydroxide, the principal factors influencing adsorption were found to be mixing time, pH and the concentrations and isoelectric points of both the virus and the absorbent. However, allophane was found to be a much better adsorbent for reovirus and R17 over the pH range 5–7, the natural pH range of many fresh waters.By using highly purified radioactive reovirus it was possible to follow both the distribution of radioactive virus in a clay suspension and the specific infectivity of the virus. This study revealed that when adsorbed virus was eluted by neutral phosphate solutions it retained its physical integrity but was of a lower specific infectivity.     

Self-purification of rivers a two-phase model for bacterial biodegradation

A simple two-phase model has been developed, which accounts for up to 95% of the total oxygen consumption during the bacterial phase of river self-purification. The two phases are characterized by a logistic model. The first corresponds roughly to a destruction of of the substrate, and is accompanied by the formation of cellular reserves. The second is the consumption of these stored substances, and begins when the external substrate ceases to be more attractive than the endogenous reserves. The cell yield as well as the stored material proportion appear to be constant, while the kinetic parameter ( ) is strongly S₀ dependent. All this can be studied through oxygen balances.     

Distribution of selected anthropogenic radionuclides (137Cs, 238Pu, (239,240)Pu and 241Am) in marine sediments with emphasis on the Spitsbergen-Bear Island area

Measurements of anthropogenic radionuclides in marine sediments can provide good estimates of past and present radioactive contamination of the marine environment. The Barents Sea is one of the world's richest ocean areas, and it is therefore of major interest for Norwegian fisheries to document the levels of radioactive contamination of this and adjacent ocean areas. In this study, concentrations of 137Cs, 238Pu, (239,240)Pu and 241Am were measured in marine sediments collected mainly in the Spitsbergen-Bear Island region. Additional samples collected in the deep Norwegian Sea and near the Greenland ice-edge have been included for comparison. The highest radionuclide levels were found near Spitsbergen, reaching over 50% higher than in adjacent areas. Determinations of the mineral content and particle size distribution indicate a relation between the content of clay minerals and radionuclide levels. The present study confirms earlier observations of elevated levels of 137Cs in sediments in the Spitsbergen-Bear Island area and documents elevated levels of 238Pu, (239,240)Pu and 241Am in these sediments. Using an exponential curve fit based on determinations of unsupported 210Pb in sediment cores, sedimentation rates of 0.28, 0.05 and 0.26 cm year(-1) were calculated at locations south of Spitsbergen, near the Greenland ice-edge and in the deep Norwegian Sea, respectively.     

A new approach for simulating aerodynamic vibrations of structures in a wind tunnel—development of an experimental system by means of hybrid vibration technique

The present paper describes a newly proposed technique for simulating aerodynamic vibration of structures, which is referred to as the hybrid vibration technique or HVT. This technique is a combination of step-by-step computer calculation and measurement of the aerodynamic force acting on a model structure in a wind tunnel test. Even though the HVT can be applied effectively, problems still exist with respect to obtaining accurate simulations. These problems include response delay in controlling the model behavior and the inertia force component present in the measured aerodynamic force. Techniques for compensating for response delay and for eliminating inertia force from measured aerodynamic force are applied to a developed system based on HVT simulations of aerodynamic vibration of elastic structures and structures exhibiting elasto-plastic behavior are performed by means of the newly developed system. The effectiveness of the techniques for compensating for the response delay and eliminating the inertia force can be confirmed from the results of the simulations. In addition, the possibility and applicability of HVT is indicated.     

A steady-state model for the rotating biological disc reactor

A steady-state model for substrate removal in a rotating biological disc reactor is presented. The model considers the consumption of substrate by micro-organisms in the biofilm attached to the rotating disc, and mass transfer from the attached liquid film to the biofilm. A mass balance on substrate over the liquid in the trough provides an expression for effluent substrate in terms of the microbial kinetic constants, the geometry of the system and the operating conditions. In order to simplify the solution of the equations in the model, first order kinetics are assumed for the rate of microbial growth and substrate utilization. This simplified model can be solved, for example, by using a programmable calculator. The model predicts that the fractional removal of substrate per stage is strongly dependent on the hydraulic loading rate per unit disc area but independent of feed substrate concentration. It predicts constant removal independent of disc size provided the hydraulic loading per unit area is kept constant. The rotational speed has only a slight predicted effect on the rate of substrate removal. The predictions of the model are compared with published data from the literature for both domestic wastewater and some industrial wastes. Trends observed in the field are predicted qualitatively by the model.     

Spatial and temporal trends of petroleum hydrocarbons in wild mussels from the Galician coast (NW Spain) affected by the Prestige oil spill

Polycyclic aromatic hydrocarbons (PAHs) were determined in tissues of wild mussels (Mytilus galloprovincialis) from the Galicia coast (NW Spain) in order to assess the extent of the environmental impact caused by the Prestige oil spill (November 13, 2002). Three sampling campaigns were carried out in February, June and November 2003 at 24 stations along the Galicia coast, from La Guardia (Pontevedra) to Ribadeo (Lugo). The spatial distribution of PAHs found in the first sampling period, clearly revealed the central area (Costa da Morte) as the most affected by the oil spill. In these stations, concentrations up to 7780 microg/kg dw of the sum of 13 parent PAHs were found 2-3 months after the spill. Molecular parameters within the aliphatic and aromatic fractions confirmed the presence of the Prestige oil in these samples. The levels markedly decreased at most of the stations in the second sampling and recovered to levels found before the spill in November 2003, 1 year after the accident (29-279 microg/kg dw, av. 133+/-83 microg/kg dw). However, a certain increase was observed in some sites which could be related to the remobilization of oil residues from still unclean intertidal spots or sediments due to the winter marine weather conditions.     

Betons a hautes performances,a granulats artificiels actifs

High strength concretes, in particular at early ages (more than 40 MPa at 3 days and up to 80 MPa at 7 days), are obtained by replacement of certain granular size ranges of sand or gravel by cement-active artificial aggregates in a given concrete composition elaborated with a local supply of aggregates and cement. The strengthening of the paste-aggregate bond by chemical reactions between the artificial aggregates and the cement also involves an improvement of other concrete performances and not only of mechanical strengths. The cracking ability is decreased, the wear strength is higher, and water permeability is low or even nil for certain compositions. The laboratory results are confirmed by tests on the site and show the possibility of achieving high strength concretes without any particular selection of cement and natural aggregates.     

Concurrent denitrification and oxygen uptake in microbial films

Mixed microbial films were grown on the inner walls of a tubular reactor with recirculation of the reactor contents and continuous flow-through of nutrient solution. The loss of total oxidized nitrogen was correlated to the film population, the nitrite concentration and the dissolved oxygen concentration in the reactor. When film population was greater than 0.5 × 10⁹ cells cm⁻², reactor dissolved oxygen concentration greater than 1 mg l⁻¹ had little effect on the nitrate loss. Nitrate loss declined for film populations greater than 2 × 10⁹ cells cm⁻². Models based on Monod and zero-order microbial kinetics were calibrated using these data and a nonlinear least squares method. There was little difference in the residual errors with these methods.     

Indirect check of aggregate pore effects on concrete

It is very difficult to quantify aggregate pores directly in a way that allows pore/water interaction to be accurately related to hardened concrete properties. This work examines, a number of aggregates through water absorption checks and gives results of tests on some concretes made with them. Three especially relevant concrete properties are examined, initial surface absorption, freeze/thaw behaviour and drying shrinkage; it is shown that aggregate absorption is reflected in these properties.