The cortisol response to psychological stress in temporomandibular dysfunction

Recombinant antibody fragments can be produced in large quantities using bacterial expression systems and could potentially be useful for the generation of biofilters for the selective removal of viral particles from fluids. A human single chain-Fv antibody library, derived from synthetic repertoires of germ line VH-gene segments rearranged in vitro and paired to a single light chain (Nissim et al., 1994, EMBO J., 13, 692-698), has recently been used to isolate hundreds of different binding specificities by panning with antigen. Antibodies from this library typically have affinities in the 10(6)-10(7) M-1 range. Occasionally, better binders are isolated but at other times the affinities recovered are poor. In the latter situation binding cannot be detected with soluble antibodies, but only by high-avidity display of multiple copies of antibodies on phage. By panning with human cytomegalovirus (HCMV)-coated immunotubes, we have isolated a number of antibody clones from this library that bound to the antigen only if displayed on the filamentous phage, but not in soluble form. One of these clones was selected for an affinity maturation procedure, achieved by combinatorial mutagenesis of the complementarity determining region 3 (CDR3) of the antibody light chain, followed by selection of the resulting library for HCMV binding. By this means, we were able to isolate a number of binders, some of which exhibited specific HCMV binding in soluble form. The clone that gave the strongest ELISA signal was expressed in bacteria, purified in solution, characterised using a novel capture methodology with surface plasmon resonance detection on a BIAcore instrument and used for the production of an immunofilter for the removal of HCMV form human serum. The filter removed more than 99% of applied HCMV in 10 min circulation time, while the amount of HCMV retained non-specifically in a cartridge derivatised with a non-specific antibody was less than 10% under similar conditions.     

星用光纤通信系统中的发光二极管的总剂量效应研究

介绍了星用光纤通信系统中的发光二极管在 6 0 Co- γ射线辐照下的总剂量效应试验研究 ,通过对不同剂量辐照情况下发光二极管的发光光谱、伏安特性及 P- I曲线进行测试 ,给出了在不同剂量的 γ- 6 0 Co射线辐照下发光二极管的发光光谱、伏安特性及 P- I曲线     

Framing as a theory of media effects

Research on framing is characterized by theoretical and empirical vagueness. This is due, in part, to the lack of a commonly shared theoretical model underlying framing research. Conceptual problems translate into operational problems, limiting the comparability of instruments and results. In this paper I systematize the fragmented approaches to framing in political communication and integrate them into a comprehensive model. I classify previous approaches to framing research along two dimensions: the type of frame examined (media frames vs. audience frames) and the way frames are operationalized (independent variable or dependent variable). I develop a process model of framing, identifying four key processes that should be addressed in future research: frame building, frame setting, individual-level processes of framing, and a feedback loop from audiences to journalists.     

钝化膜提高GaN基LED光提取效率研究

模拟计算了光的入射角度与反射率的关系,当光的入射角度大于23°时,发生全反射,无论是否在器件表面生长增透膜,这时的光都无法从器件顶部出射表面提取出来。研究了使用等离子体增强化学气相沉积法(PECVD)在已经制备了n电极和p电极的GaN基LED上制备钝化膜,分析了SiON和SiN_x膜沉积对于器件的光输出功率的影响。通过实验证明,在器件上沉积SiON后,光输出功率增加。     

偏心孔缝箱体屏蔽效能电磁拓扑分析算法

基于电磁拓扑理论,对Robinson等效电路模型进行改进,提出了箱体屏蔽效能分析的电磁拓扑算法.该算法将孔缝等效为二端口网络,通过散射矩阵准确地描述了箱体内部场的行驻波态,实现了内外场耦合的准确建模;结合偏心孔缝箱体分析理论,求解偏心孔缝散射矩阵,进而实现了偏心孔缝箱体的屏蔽效能分析.在0～2 GHz频域内,分别对偏心单孔和孔阵箱体的屏蔽效能进行了仿真,通过与软件CST数值结果的对比,验证了算法的有效性.最后采用该算法详细分析了孔缝宽度、测试点距离、入射极化角和入射方位角对单孔箱体屏蔽效能的影响.     

分布式并行数据挖掘计算框架及其算法研究

为了提供一个灵活可扩展的计算平台进行高效的挖掘计算，提出了一种应用于分布和并行环境的数据挖掘计算框架和相应的算法。通过分析关联规则挖掘理论和以往算法的优缺点，建立一种分布式并行数据挖掘的计算框架，并给出相应的求解算法。实例分析表明该计算框架能够减少节点问的通信开销，保持了良好的可扩展性：挖掘算法则利用本地节点动态有序集合枚举树生成方法代替数据库节省了本地空间的占用．大大提高了查找的计算效率。     

OFDM系统中自适应调制和功率分配方案

为了提高Ka频段正交频分复用(OFDM)系统的频谱效率,提出了一种子带自适应调制和功率分配算法。该算法在目标误比特率下首先通过等功率分配确定各子带的初始信噪比大小和调制方式,并根据子带信噪比和调制方式的关系,迭代运算出最优的有效子带个数、调制方案和功率分配的比例关系。仿真结果表明,该算法提高了系统中低信噪比下的频谱效率。     

基于多参数加权分数阶傅里叶变换的星座预编码系统研究与实现

针对当前多参数加权分数阶傅里叶变换(Multiple Parameters Weighted-type FRactional Fourier Transform, MP-WFRFT)星座特征缺乏定量研究的缺限,该文提出一种新型的星座叠加数学模型。通过分别研究信号的时域及频域分量,给出了星座模糊及裂变的定性及定量分析结论,并搭建了基于MP-WFRFT的星座预编码系统。通过构建相关混合整数优化模型,并利用遗传算法进行模型优化,进而设计出符合预期通信需求的预编码系统。经仿真分析,验证了所提出的星座叠加数学模型及优化方案的正确性;同时通过USRP硬件平台测试表明了所搭建的基于MP-WFRFT星座预编码系统的可行性。     

基于方位-能量的海底声学参数估计方法研究

估计海底声学参数并应用的方法有很多种,但由于过程复杂、投入较大,大都不容易实施.文中利用建立的方位-能量估计海底参数模型,在预先给出目标距离的情况下,使用遗传算法估计海底声学参数.这使得潜艇自身的声纳既可以用于在平时训练中积累海区海底声学参数又可以在战时直接使用这些参数为作战服务.这样海底声学参数的估计与应用有机的结合为一个整体,使得整个过程简便快捷、易于实现.     

被动声纳探测距离空间分形特征研究

根据分形理论,借助于GIS技术的支持,以一定优质因子条件下声纳探测距离为例,以声纳探测距离等值线为研究对象,对以往研究少有涉及的声纳探测距离等值线的分形性质进行了探讨,得出声纳探测距离等值线具有分形特征的结论,为描述声纳探测距离提供了新的参数,从而为声纳探测距离的预报提供了一种新的理论方法.