Additional modules for versatile and economical PCR-based gene deletion and modification in Saccharomyces cerevisiae

An important recent advance in the functional analysis of Saccharomyces cerevisiae genes is the development of the one-step PCR-mediated technique for deletion and modification of chromosomal genes. This method allows very rapid gene manipulations without requiring plasmid clones of the gene of interest. We describe here a new set of plasmids that serve as templates for the PCR synthesis of fragments that allow a variety of gene modifications. Using as selectable marker the S. cerevisiae TRP1 gene or modules containing the heterologous Schizosaccharomyces pombe his5⁺ or Escherichia coli kan^r gene, these plasmids allow gene deletion, gene overexpression (using the regulatable GAL1 promoter), C- or N-terminal protein tagging [with GFP(S65T), GST, or the 3HA or 13Myc epitope], and partial N- or C-terminal deletions (with or without concomitant protein tagging). Because of the modular nature of the plasmids, they allow efficient and economical use of a small number of PCR primers for a wide variety of gene manipulations. Thus, these plasmids should further facilitate the rapid analysis of gene function in S. cerevisiae. © 1998 John Wiley & Sons, Ltd.     

SAR image denoising via Bayesian wavelet shrinkage based on heavy-tailed modeling

Synthetic aperture radar (SAR) images are inherently affected by multiplicative speckle noise, which is due to the coherent nature of the scattering phenomenon. This paper proposes a novel Bayesian-based algorithm within the framework of wavelet analysis, which reduces speckle in SAR images while preserving the structural features and textural information of the scene. First, we show that the subband decompositions of logarithmically transformed SAR images are accurately modeled by alpha-stable distributions, a family of heavy-tailed densities. Consequently, we exploit this a priori information by designing a maximum a posteriori (MAP) estimator. We use the alpha-stable model to develop a blind speckle-suppression processor that performs a nonlinear operation on the data and we relate this nonlinearity to the degree of non-Gaussianity of the data. Finally, we compare our proposed method to current state-of-the-art soft thresholding techniques applied on real SAR imagery and we quantify the achieved performance improvement.     

Improving Point Cloud Accuracy Obtained from a Moving Platform for Consistent Pile Attack Pose Estimation

We present a perception system for enabling automated loading with waist-articulated wheel loaders. To enable autonomous loading of piled materials, using either above-ground wheel loaders or underground load-haul-dump vehicles, 3D data of the pile shape is needed. However, using common 3D scanners, the scan data is distorted while the wheel loader is moving towards the pile. Existing methods that make use of 3D scan data (for autonomous loading as well as tasks such as mapping, localisation, and object detection) typically assume that each 3D scan is accurate. For autonomous robots moving over rough terrain, it is often the case that the vehicle moves a substantial amount during the acquisition of one 3D scan, in which case the scan data will be distorted. We present a study of auto-loading methods, and how to locate piles in real-world scenarios with nontrivial ground geometry. We have compared how consistently each method performs for live scans acquired in motion, and also how the methods perform with different view points and scan configurations. The system described in this paper uses a novel method for improving the quality of distorted 3D scans made from a vehicle moving over uneven terrain. The proposed method for improving scan quality is capable of increasing the accuracy of point clouds without assuming any specific features of the environment (such as planar walls), without resorting to a “stop-scan-go” approach, and without relying on specialised and expensive hardware. Each new 3D scan is registered to the preceding using the normal-distributions transform (NDT). After each registration, a mini-loop closure is performed with a local, per-scan, graph-based SLAM method. To verify the impact of the quality improvement, we present data that shows how auto-loading methods benefit from the corrected scans. The presented methods are validated on data from an autonomous wheel loader, as well as with simulated data. The proposed scan-correction method increases the accuracy of both the vehicle trajectory and the point cloud. We also show that it increases the reliability of pile-shape measures used to plan an efficient attack pose when performing autonomous loading.     

Modelling,validating, and ranking of secure service compositions

In the world of large‐scale applications, software as a service (SaaS) in general and use of microservices, in particular, is bringing service‐oriented architectures to a new level: Systems in general and systems that interact with human users (eg, sociotechnical systems) in particular are built by composing microservices that are developed independently and operated by different parties. At the same time, SaaS applications are used more and more widely by enterprises as well as public services for providing critical services, including those processing security or privacy of relevant data. Therefore, providing secure and reliable service compositions is increasingly needed to ensure the success of SaaS solutions. Building such service compositions securely is still an unsolved problem. In this paper, we present a framework for modelling, validating, and ranking secure service compositions that integrate both automated services as well as services that interact with humans. As a unique feature, our approach for ranking services integrates validated properties (eg, based on the result of formally analysing the source code of a service implementation) as well as contractual properties that are part of the service level agreement and, thus, not necessarily ensured on a technical level.     

Daily impaired detachment and short-term effects of impaired sleep quality on next-day commuting near-accidents – an ambulatory diary study

This study investigated the short-term effects of daily recovery, that is, impaired psychological detachment from work and various actigraphical indicators of sleep quality, on near-accidents when commuting to work the next morning. Furthermore, the mediating effect of actigraphically assessed sleep quality on the relationship between impaired psychological detachment from work and near-accidents when commuting to work was analysed. Fifty-six full-time employees of a Swiss assurance company participated in the one-week study. Multilevel analyses revealed that impaired detachment was highly related to a decrease in sleep duration. Furthermore, impaired daily recovery processes, such as impaired psychological detachment from work and disturbed sleep quality, were related to commuting near-accidents. Impaired sleep quality mediated the effect of impaired psychological detachment from work on these near-accidents. Our results show that occupational safety interventions should address both impaired psychological detachment from work and sleep quality in order to prevent near accidents when commuting to work.

Practitioner Summary: Commuting accidents occur frequently and have detrimental effects on employees, organisations and society. This study shows that daily lack of recovery, that is, impaired psychological detachment and impaired sleep quality, is related to near-accidents when commuting to work the next morning. Primary prevention of commuting accidents should therefore address daily lack of recovery.     

Patterns in Performance-Orientated Design: An Approach towards Pattern Recognition,Generation and Instrumentalisation

The question of pattern in architecture generally divides architects into two distinct groups: those with an aesthetic interest in man-made ornament; and those who take a deeper interest in the processes that underlie the formation of pattern in the natural world. An investigation of the performative, though, enables an exploration of pattern that arises out of the interaction of man-made interventions with the natural environment. Michael Hensel , Professor for research by Design at AHO, the Oslo School of Architecture and Design, and Achim Menges of the Emtech (Emergent Technologies and Design programme) at the Architectural Association in London, explore this arena based on their research and educational work at the AA and other international institutions, which focuses on aspects of performance in the built environment. Copyright © 2009 John Wiley & Sons, Ltd.     

Field-deployable, high-resolution, time-of-flight aerosol mass spectrometer

The development of a new high-resolution time-of-flight aerosol mass spectrometer (HR-ToF-AMS) is reported. The high-resolution capabilities of this instrument allow the direct separation of most ions from inorganic and organic species at the same nominal m/z, the quantification of several types of organic fragments (CxHy, CxHyOz, CxHyNp, CxHyOzNp), and the direct identification of organic nitrogen and organosulfur content. This real-time instrument is field-deployable, and its high time resolution (0.5 Hz has been demonstrated) makes it well-suited for studies in which time resolution is critical, such as aircraft studies. The instrument has two ion optical modes: a single-reflection configuration offers higher sensitivity and lower resolving power (up to approximately 2100 at m/z 200), and a two-reflectron configuration yields higher resolving power (up to approximately 4300 at m/z 200) with lower sensitivity. The instrument also allows the determination of the size distributions of all ions. One-minute detection limits for submicrometer aerosol are <0.04 microg m(-3) for all species in the high-sensitivity mode and <0.4 microg m(-3) in the high-resolution mode. Examples of ambient aerosol data are presented from the SOAR-1 study in Riverside, CA, in which the spectra of ambient organic species are dominated by CxHy and CxHyOz fragments, and different organic and inorganic fragments at the same nominal m/z show different size distributions. Data are also presented from the MIRAGE C-130 aircraft study near Mexico City, showing high correlation with independent measurements of surrogate aerosol mass concentration.