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31.
The development of a steady-state process model for a numerical study of transport phenomena in the fusion zone of deep penetration laser beam welded joints is described. Based on the conservation equations of energy, momentum, and mass in dimensionless form, the three-dimensional modeling considers viscous, buoyancy, and friction forces as well as surface tension gradients. The developed simulation program enables the prediction of the local temperature and velocity distribution in the molten material. Consequently, the resultant weld pool dimensions dependent on the characteristic dimensionless groups can be derived. 相似文献
32.
The race for creating an automated patch clamp has begun. Here, we present a novel technology to produce true gigaseals and whole cell preparations at a high rate. Suspended cells are flushed toward the tip of glass micropipettes. Seal, whole-cell break-in, and pipette/liquid handling are fully automated. Extremely stable seals and access resistance guarantee high recording quality. Data obtained from different cell types sealed inside pipettes show long-term stability, voltage clamp and seal quality, as well as block by compounds in the pM range. A flexible array of independent electrode positions minimizes consumables consumption at maximal throughput. Pulled micropipettes guarantee a proven gigaseal substrate with ultra clean and smooth surface at low cost. 相似文献
33.
Joachim Mittendorf Prof. Dr. Stefan Weigand Dr. Cristina Alonso‐Alija Dr. Erwin Bischoff Dr. Achim Feurer Dr. Michael Gerisch Dr. Armin Kern Dr. Andreas Knorr Dr. Dieter Lang Dr. Klaus Muenter Dr. Martin Radtke Dr. Hartmut Schirok Dr. Karl‐Heinz Schlemmer Dr. Elke Stahl Dr. Alexander Straub Dr. Frank Wunder Dr. Johannes‐Peter Stasch Dr. 《ChemMedChem》2009,4(5):693-693
34.
Operational remote monitoring of snowpack stratigraphy, melt water intrusions and their evolution with time for forecasting snowpack stability is not possible to date. Determination of the spatial variability of snowpack conditions on various scales requires a number of point measurements with various methods. These methods are either destructive or do not provide information about the internal structure of the snowpack. The application of a remotely controlled non-destructive sensor system would help to gain a higher spatio-temporal resolution about information of the snowpack. In this study we present results from upward-looking ground-penetrating radar (GPR) surveys from horizontal caves dug in the front wall of snow pits at the bottom of the snowpack. GPR data are compared with vertical profiles of snow hardness and density, obtained in the snow pit. Data were acquired in different areas with varying snow conditions with various GPR impulse systems, frequencies and polarizations. Radar experiments with high frequencies (> 1 GHz) detect internal layers in the snowpack in dry snow, but fail to provide clear reflections at the upper snow-air transition because of attenuation. In wet snow, the radar signals < 1 GHz are capable to penetrate a meter-thick snowpack and detect the snow surface, although the signal is strongly attenuated. Analysis of reflection phases and magnitudes allows interpretation of their physical origin in terms of changes in electrical permittivity. Varying antenna polarization causes a strongly different signal response, likely caused by the snow-pit wall present in our set-up. Forward calculation of density-based reflection coefficients between neighboring layers of varying hardness yields ambiguous results in terms of correspondence with observed radar reflections apart except for interferences of neighboring reflections. Moreover, we identify several pitfalls for future applications. The system set-up used here represents a basis for further developments towards a system, which is capable of improving information on the spatial and temporal snowpack characteristics. 相似文献
35.
Achim Leitzke 《臭氧:科学与工程》2009,31(4):301-308
The rate constants of ozone with acrylic, methacrylic, fumaric, maleic, muconic and dichloromaleic acids in aqueous solution were determined. Substituent effects and state of protonation strongly influence these rate constants. The reactions follow largely the Criegee mechanism (formation of carbonyl compound plus hydroxyalkylhydroperoxide). There is a pronounced regioselectivity in the decay reactions of the ozonide. Depending on their structures, the hydroxyhydroperoxidic intermediates decay by various routes: decarboxylation, H2O2 elimination or water elimination. Preliminary data on dicloromaleic acid indicate than an additional decay leading to chlorine atoms route must be envisaged. 相似文献
36.
Prof. Dr. Hans Ulrich Buhl Dr. Bernd Heinrich Prof. Dr. Peter Loos Prof. Dr. Ulrich Frank Visiting Prof. Daniel L. Moody PhD Prof. Jeffrey Parsons PhD Prof. Dr. Michael Rosemann Prof. Dr. Elmar J. Sinz Prof. Ron Weber PhD Achim Kindler Dr. Prof. Dr. Claus Rautenstrauch Dipl.-Wirt.-Inf. Peter Fettke 《WIRTSCHAFTSINFORMATIK》2005,47(2):152-161
37.
Breunig M Lungwitz U Klar J Kurtz A Blunk T Goepferich A 《Journal of nanoscience and nanotechnology》2004,4(5):512-520
For non-viral gene delivery, the carriers for DNA transfer into cells must be vastly improved. The branched cationic polymer polyethylenimine has been described as an efficient gene carrier. However, polyethylenimine was demonstrated to mediate substantial cytotoxicity. Therefore, this study is aimed at investigating per-N-methylated polyethylenimine, which is thought to have a much lower cytotoxicity due to its lower charge density. Results from a gel retardation assay and laser light scattering indicated that per-N-methylated polyethylenimine condenses DNA into small and compact nanoparticles with a mean diameter <150 nm. Furthermore, polyplexes of polyethylenimine and per-N-methylated polyethylenimine with DNA had a positive zeta potential and the polymers protected DNA from nuclease-mediated digestion. The transfection efficiency of polyethylenimine and per-N-methylated polyethylenimine was tested in CHO-K1 cells. Using green fluorescent protein as reporter gene and flow cytometry analysis, we demonstrated that per-N-methylated polyethylenimine has a lower cytotoxicity, but also a significantly lower transfection efficiency. Using propidium iodide staining, we could additionally distinguish between viable and dead cells. At NP > or = 12, per-N-methylated polyethylenimine showed a much higher cell viability and the ratio of viable and transfected cells to dead and transfected cells was about 1.5 to 1.7 fold higher than for polyethylenimine. The results of cell viability from flow cytometry analysis were confirmed by the MTS assay. Using luciferase reporter gene for transfection experiments, the gene expression of per-N-methylated polyethylenimine was lower at NP 6, 12 and 18 as compared to polyethylenimine, but at NP 24 it yielded similar levels. 相似文献
38.
The long-term variability of the fetal heart rate (FHR) provides valuable information on the fetal health status. The routine clinical FHR measurements are usually carried out by the means of ultrasound cardiography. Although the frequent FHR monitoring is recommendable, the high quality ultrasound devices are so expensive that they are not available for home care use. The passive and fully non-invasive acoustic recording called phonocardiography, provides an alternative low-cost measurement method. Unfortunately, the acoustic signal recorded on the maternal abdominal surface is heavily loaded by noise, thus the determination of the FHR raises serious signal processing issues. The development of an accurate and robust fetal phonocardiograph has been since long researched. This paper presents a novel two-channel phonocardiographic device and an advanced signal processing method for determination of the FHR. The developed system provided 83% accuracy compared to the simultaneously recorded reference ultrasound measurements. 相似文献
39.
Aggregate architectures are full-scale spatial formations made from loose granular matter. Especially if the individual grain is custom-designed, the range of behaviours can be calibrated to match a wide range of architectural and structural performance criteria. The aggregate becomes programmable matter. The relevance of loose granular systems for architecture is on the one hand their rapid re-configurability, allowing for a system not to be destroyed but rather to be recycled. On the other hand aggregates per se can be functionally graded either within one and the same particle type or through mixing different particle geometries. This enables the variation of architectural properties throughout one and the same material system, which is one of the core postulates of current architectural design research. However, very few examples of designed granular matter in architecture exist. The results presented here are thus one of the first coherent bodies of comprehensive research in this field compiled over a period of five years. Methodologically aggregate systems challenge conventional architectural design principles: whereas an architect generally precisely defines local and global geometry of a structure, in a designed granular system he can only calibrate the particle geometry in order to tune the overall behaviour of the aggregate formation. Thus new design methods have been developed throughout the research projects, which are informed by the related fields of granular physics and behaviour-based robotics. In this context the article provides an introduction to both designed particle systems and suitable fabrication approaches in an architectural context. Case study projects serve to verify the applicability of the concepts introduced. The research findings are discussed with regards to their practical, methodological and design theoretical contributions. To conclude, further directions of research are highlighted. 相似文献
40.
Codifying expert domain knowledge is a difficult and expensive task. To evaluate the quality of the outcome, often the same domain expert or a colleague of similar expertise is relied on to undertake a direct evaluation of the knowledge-based system or indirectly by preparing appropriate test data. During an incremental knowledge acquisition process, a data stream is available, and the knowledge base is observed and amended by an expert each time it produces an error. Using the kept record of the system’s performance, we propose an evaluation process to estimate its effectiveness as it gets evolved. We instantiate this process for an incremental knowledge acquisition methodology, Ripple Down Rules. We estimate the added value in each knowledge base update. Using these values, the decision makers in the organisation employing the knowledge-based information system can apply a cost-benefit analysis of the continuation of the incremental knowledge acquisition process. They can then determine when this process, involving keeping an expert online, should be terminated. As a result, the expert is not kept on-line longer than it is absolutely necessary. Hence, a major expense in deploying the information system—the cost of keeping a domain expert on-line—is reduced. 相似文献