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41.
Priego-Capote F Ramirez-Boo M Hoogland C Scherl A Mueller M Lisacek F Sanchez JC 《Analytical chemistry》2011,83(14):5673-5680
Despite continuous advances in hyperglycemia treatments, a precise control through monitoring of glucose and glycated hemoglobin remains in most diabetic patients as the diagnosis/prognosis tool. An alternative perspective could be the discovery and quantitation of new blood glycated proteins formed by nonenzymatic reaction with circulatory glucose. As a result, the human hemolysate is an incomparable source of glycated proteins to further monitor glycemia and interpret changes at the level of this post-translational modification. The human hemolysate is here studied based on the differential labeling of proteins with isotopically labeled-glucose ([(13)C(6)] glucose), named glycation isotopic labeling. Due to the chemoselectivity of glycation, only preferential targets are labeled by this protocol. The approach provides qualitative data through the detection of preferential protein glycation sites as well as quantitative information to evaluate the abundance of this modification. This strategy was applied to human hemolysate samples corresponding to different glycemic states estimated by laboratory-certified concentrations of glycated hemoglobin. The glycation level of each protein can then be employed to interpret the effect of glucose exposition as a consequence of glycemic unbalance. This information should provide new molecular insights into protein glycation mechanisms that might generate a new hypothesis to clinicians to improve the understanding of underlying pathologies associated to prolonged hyperglycemia. 相似文献
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Polycrystalline Fe7.5Al7Cr is a ferritic light weight steel that shows a strong anisotropic dissolution behaviour. This electrochemical phenomenon was investigated by use of a scanning droplet cell (SDC). The crystallographic orientation of the single grains was determined by electron back scatter diffraction (EBSD). The fraction of grains with a near 〈1 1 1〉 orientation (<10°) was 1.88 times higher than that of the near 〈0 0 1〉 orientation prior to annealing and 3.58 times higher after annealing. Voltammograms were performed on single grains with low index planes. The critical current density was 53% higher on 〈0 0 1〉 grains as compared to 〈1 1 1〉 grains. These results are used to discuss the electrochemical behaviour of the polycrystalline FeAlCr. 相似文献
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Agnes M Mwangwela Ralph D Waniska Cassandra McDonough Amanda Minnaar 《Journal of the science of food and agriculture》2007,87(3):399-410
Bechuana white cowpeas were micronised to three temperatures (130, 153 and 170 °C). Cooking properties of the cowpea seeds and the role of starch‐related properties were studied. In all cases, micronisation significantly reduced the cooking time and thus the time required for the cowpea seeds to attain a suitably soft texture. This was attributed in part to the significant improvement in rate of water absorption during cooking and starch pre‐gelatinisation, as evidenced by loss of birefringence and increased susceptibility of the cowpea starch to α‐amylase digestion. However, micronisation to 170 °C resulted in a severe deterioration in pasting properties of the cowpea flour, possibly due to starch depolymerisation and/or amylose‐associated crosslinking. Owing to these changes, cowpea seeds micronised to 170 °C required a longer cooking time than the other two micronised samples. Flour prepared from cowpea seeds micronised to 170 °C may have limited starch functionality. Copyright © 2006 Society of Chemical Industry 相似文献
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The principles for control of cracking in concrete structures due to imposed strain are investigated in this paper. The effects of uniform and non-uniform cooling and shrinkage are studied in medium-thick walls with regard to the amount of reinforcement necessary to distribute cracks. The influence of reinforcement on the behavior after the first crack is investigated, in particular its ability to reduce crack widths. The restraint force is significantly reduced for non-uniformly distributed imposed strain due to softening and cracking in the concrete. The amount of minimum reinforcement needed to distribute cracks is therefore significantly smaller for imposed strain in the form of drying shrinkage than for thermal imposed strain. Crack reinforcement is most effective for structures with large dimensions in the direction of external restraint. 相似文献
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Inhalation exposure to particles <10 microm in size that are suspended in the troposphere (PM10) is a factor in respiratory and cardiovascular diseases. The extent of the injury, local to systemic inflammation, is dependent on the number, size, and composition of the particles to which an individual is exposed. The physical properties of and compounds on PM10 that are responsible for these adverse effects on human health are the subject of intense investigation. Here, we report a laboratory method that involved the creation of 1-120 particles per trial that were of known size and composition, followed by deposition of them directly onto individual human lung cells within a cell culture, and after an incubation period, a downstream biological response was measured. To illustrate this methodology, particles that each contained 50 pg of lipopolysaccharide were created and deposited onto individual cells over a region <0.36 mm2 within a genetically modified A549 cell culture. The biological readout was the relative expression of intercellular cell adhesion molecule 1 after 24 h of incubation using an immunocytochemistry assay. The apparatus and methodology introduced here enables studies at the interface between the relevant but diverse areas of atmospheric particle chemistry and lung cell biology to identify the chemical and physical factors of PM10 that cause/exacerbate respiratory and cardiovascular diseases by triggering various biological pathways. 相似文献