全文获取类型
收费全文 | 1098篇 |
免费 | 89篇 |
国内免费 | 1篇 |
专业分类
电工技术 | 7篇 |
化学工业 | 673篇 |
金属工艺 | 19篇 |
机械仪表 | 14篇 |
建筑科学 | 36篇 |
矿业工程 | 5篇 |
能源动力 | 19篇 |
轻工业 | 198篇 |
水利工程 | 4篇 |
无线电 | 21篇 |
一般工业技术 | 117篇 |
冶金工业 | 36篇 |
原子能技术 | 1篇 |
自动化技术 | 38篇 |
出版年
2024年 | 2篇 |
2023年 | 20篇 |
2022年 | 153篇 |
2021年 | 207篇 |
2020年 | 48篇 |
2019年 | 44篇 |
2018年 | 41篇 |
2017年 | 54篇 |
2016年 | 46篇 |
2015年 | 44篇 |
2014年 | 56篇 |
2013年 | 60篇 |
2012年 | 60篇 |
2011年 | 63篇 |
2010年 | 52篇 |
2009年 | 48篇 |
2008年 | 33篇 |
2007年 | 25篇 |
2006年 | 23篇 |
2005年 | 13篇 |
2004年 | 17篇 |
2003年 | 7篇 |
2002年 | 14篇 |
2001年 | 5篇 |
2000年 | 5篇 |
1999年 | 7篇 |
1998年 | 8篇 |
1997年 | 6篇 |
1996年 | 5篇 |
1995年 | 5篇 |
1993年 | 5篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1984年 | 1篇 |
1977年 | 1篇 |
1976年 | 5篇 |
1974年 | 1篇 |
排序方式: 共有1188条查询结果,搜索用时 0 毫秒
21.
Mateusz Sypniewski Zbigniew J. Krl Joanna Szyda Elbieta Kaja Magdalena Mroczek Tomasz Suchocki Adrian Lejman Maria Stpie Piotr Topolski Maciej Dbrowski Krzysztof Kotlarz Angelika Aplas Micha Wasiak Marzena Wojtaszewska Pawe Zawadzki Agnieszka Pawlak Robert Gil Paula Dobosz Joanna Stojak 《International journal of molecular sciences》2022,23(15)
Background: Severe outcomes of COVID-19 account for up to 15% of all cases. The study aims to check if any gene variants related to cardiovascular (CVD) and pulmonary diseases (PD) are correlated with a severe outcome of COVID-19 in a Polish cohort of COVID-19 patients. Methods: In this study, a subset of 747 samples from unrelated individuals collected across Poland in 2020 and 2021 was used and whole-genome sequencing was performed. Results: The GWAS analysis of SNPs and short indels located in genes related to CVD identified one variant significant in COVID-19 severe outcome in the HADHA gene, while for the PD gene panel, we found two significant variants in the DRC1 gene. In this study, both potentially protective and risk variants were identified, of which variants in the HADHA gene deserve the most attention. Conclusions: This is the first study reporting the association between the HADHA and DRC1 genetic variants and COVID-19 severe outcome based on the cohort WGS analysis. Although all the identified variants are localised in introns, they may be correlated and therefore inherited along with other risk variants, potentially causative to severe outcome of COVID-19 but not discovered yet. 相似文献
22.
Britt Van Renterghem Agnieszka Wozniak Patricia Garrido Castro Patrick Franken Nora Pencheva Raf Sciot Patrick Schffski 《International journal of molecular sciences》2022,23(14)
Doxorubicin (doxo) remains the standard of care for patients with advanced soft tissue sarcoma (STS), even though response rates to doxo are only around 14% to 18%. We evaluated enapotamab vedotin (EnaV), an AXL-specific antibody-drug conjugate (ADC), in a panel of STS patient-derived xenografts (PDX). Eight models representing multiple STS subtypes were selected from our STS PDX platform (n = 45) by AXL immunostaining on archived passages. Models were expanded by unilateral transplantation of tumor tissue into the left flank of 20 NMRI nu/nu mice. Once tumors were established, mice were randomized into an EnaV treatment group, or a group treated with isotype control ADC. Treatment efficacy was assessed by tumor volume evaluation, survival analysis, and histological evaluation of tumors, and associated with AXL expression. EnaV demonstrated significant tumor growth delay, regression, and/or prolonged survival compared to isotype control ADC in 5/8 STS PDX models investigated. Experimental passages of responding models were all found positive for AXL at varying levels, but no linear relationship could be identified between the level of expression and level of response to EnaV. One model was found negative for AXL on experimental passage and did not respond to EnaV. This study provides a preclinical rationale for the evaluation of AXL-targeting ADCs in the treatment of AXL-expressing sarcomas. 相似文献
23.
Oliwia Metryka Daniel Wasilkowski Agnieszka Mrozik 《International journal of molecular sciences》2022,23(9)
Although the molecular response of bacteria exposed to metal nanoparticles (NPs) is intensively studied, many phenomena related to their survival, metal uptake, gene expression and protein production are not fully understood. Therefore, this work aimed to study Ag-NPs, Cu-NPs, ZnO-NPs and TiO2-NPs-induced alterations in the expression level of selected oxidative stress-related genes in connection with the activity of antioxidant enzymes: catalase (CAT), peroxidase (PER) and superoxide dismutase (SOD) in Escherichia coli, Bacillus cereus and Staphylococcus epidermidis. The methodology used included: the extraction of total RNA and cDNA synthesis, the preparation of primers for selected housekeeping and oxidative stress genes, RT-qPCR reaction and the measurements of CAT, PER and SOD activities. It was established that the treatment of E. coli and S. epidermidis with NPs resulted mainly in the down-regulation of targeted genes, whilst the up-regulation of genes was confirmed in B. cereus. The greatest differences in the relative expression levels of tested genes occurred in B. cereus and S. epidermidis treated with TiO2-NPs, while in E. coli, they were observed under ZnO-NPs exposure. The changes found were mostly related to the expression of genes encoding proteins with PER and CAT-like activity. Among NPs, ZnO-NPs and Cu-NPs increased the activity of antioxidants in E. coli and B. cereus. In turn, TiO2-NPs had a major effect on enzymes activity in S. epidermidis. Considering all of the collected results for tested bacteria, it can be emphasised that the impact of NPs on the antioxidant system functioning was dependent on their type and concentration. 相似文献
24.
25.
26.
Véronique Amstutz Alexandros Katsaounis Agnieszka Kapalka Christos Comninellis Kai M. Udert 《Journal of Applied Electrochemistry》2012,42(9):787-795
Recent studies have shown that electrolysis can be an efficient process for nitrogen removal from urine. These studies have been conducted with urea solutions or fresh urine, but urine collected in NoMix toilets and urinals has a substantially different composition, because bacteria hydrolyse urea quickly to ammonia and carbonate. In this study, we compared electrochemical removal of nitrogen from synthetic solutions of fresh and stored urine using IrO2 anodes. We could show that in fresh urine both ammonia and urea are efficiently eliminated, mainly through chlorine-mediated oxidation. However, in stored urine the presence of carbonate, arising from urea hydrolysis, leads to an inhibition of ammonia oxidation. We suggest two parallel mechanisms to explain this effect: the competition between chloride and carbonate oxidation at the anode and the competition between chlorate formation, enhanced by the buffering effect of carbonate, and ammonia oxidation for the consumption of active chlorine in the bulk. However, further experiments are needed to support the latter mechanism. In conclusion, this study highlights the negative consequences of the presence of carbonate in urine solutions, but also in other wastewaters, when subjected to an electrolytic treatment on IrO2 in alkaline media. 相似文献
27.
Agnieszka Owczarek‐Fendor An Vermeulen Ilse Van Bree Markus Eriksson Stefaan Lescouhier Stefaan De Smet Bruno De Meulenaer Frank Devlieghere 《International Journal of Food Science & Technology》2014,49(4):1090-1098
The objective of this study was to evaluate the influence of two different vacuum ageing times (7 and 14 days) and the impact of the modified atmosphere packaging (MAP) configuration (gas/product ratios: 0.5 and 1 and gas composition: 70% O2 + 30% CO2 and 40% O2 + 30% CO2 + 30% N2) on the quality of fresh beef during subsequent storage at 4 °C. For this purpose, three separate experiments were performed. For each experiment, two different muscles (Longissimus dorsi and Biceps femoris) were sampled from four double‐muscled Belgian Blue beef carcasses. Next to colour, also the evolution in microbial load, pH, O2 and CO2 in the headspace and lipid oxidation at the meat surface were evaluated. A vacuum ageing for 14 days compared with 7 days resulted in a higher initial microbial load on the day of MAP packaging, which resulted finally in a significantly shorter shelf life. This ageing effect was less pronounced on the colour stability and lipid oxidation of the meat samples. No significant influence of the packaging configuration on any of the analysed parameters (colour, microbial load, pH and lipid oxidation at the meat surface) was observed. 相似文献
28.
Katarzyna Matkiewicz Agnieszka Bukowska Wiktor Bukowski 《Journal of Inorganic and Organometallic Polymers and Materials》2012,22(2):332-341
Hydroxyl functionalized gel type resins derived from 2-hydroxyethyl, 2-hydroxypropyl and 2-hydroxy-3-phenoxypropyl methacrylates
were applied to covalent immobilization of Mn(salen) complexes via glutarate and carbonate linkers. The obtained catalysts
were characterized by FTIR and DR UV–Vis methods and elemental analysis. It was found that the activity of polymeric catalysts
obtained in this way depended on both the nature of the functional and cross-linking monomers and the degree of the cross-linking
of polymer matrices and the way of complex immobilization. The highest activity was observed for the complexes immobilized
on the HEMA resins cross-linked with 3%-mol of diethylene glycol dimethacrylate. For instance, in the presence of the catalysts
with (S,S)-salen ligand S-epoxystyrene and (1S, 2R)-epoxyindene could be obtained with 46% and 69% ee, respectively. Attempts
have also been made to recycle the selected catalyst. However, a rapid fall off in the activity was observed, although the
selectivity was similar in which three cycles. 相似文献
29.
30.
Ultrastructural and developmental toxicity of potato and tomato leaf extracts to beet armyworm,Spodoptera exigua (lepidoptera: noctuidae) 下载免费PDF全文
Zbigniew Adamski Katarzyna Radtke Agnieszka Kopiczko Szymon Chowański Paweł Marciniak Monika Szymczak Marta Spochacz Patrizia Falabella Filomena Lelario Laura Scrano Sabino A. Bufo 《Microscopy research and technique》2016,79(10):948-958
Beet Armyworm, Spodoptera exigua is a herbivorous moth and a serious pest of many economically important plants, which are used as food sources. Because of rigorous standards of food quality, usage of synthetic insecticides in crop protection, against pests, is limited. Solanaceae plant extracts may be a relatively cheap source of efficient natural insecticides that can limit usage of synthetic substances. Their biological activity is not fully known. In particular, ultrastructural studies, using transmission electron microscopy, are not usual. In the present article we describe the effects of sublethal concentrations of tomato and potato leaf extracts against S. exigua. Acute lethal effects were not observed. Both extracts exerted similar effects within midgut and fat body cells. Midgut cells were not significantly altered while fat body cells showed prominent swelling of nuclear envelope and endoplasmic reticulum, vacuolization of mitochondria and fusion of fat droplets. These changes were much more intensive within groups exposed to potato than tomato extracts at highest concentration at least. Light microscopy was used to observe and document developmental alterations of S. exigua exposed to potato and tomato leaf extracts. Potato leaf extracts significantly decreased hatching success and caused morphological malformations of imagoes. Among them, malformations of wings were the most prominent. Interestingly, these effects were not observed within populations exposed to tomato extracts at highest concentration at least. 相似文献