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101.
Blood infected with human or rodent malaria parasites, Plasmodium falciparum or Plasmodium berghei, was exposed to higher pH, higher PO2, and lower temperature than those used in standard cultivation conditions. Parasitized blood was incubated for 20, 25, and 30 min with RPMI 1640 medium, 10% (vol/vol) serum, pH 8.0, at 20 degrees C in the air, conditions which are ultimately lethal to the asexual stages of malarial parasites. Markedly dilated clefts were observed in the cytoplasm of the malaria-infected erythrocytes so treated. These clefts can take up colloidal gold particles and macromolecules such as Protein A, rhodamine-dextran, and lucifer yellow-dextran. Such dilated clefts were not seen in the cytoplasm of infected erythrocytes that were incubated under normal cultivation conditions before fixation. These had slender clefts of the usual sort that did not take up colloidal gold particles and macromolecules. 相似文献
102.
103.
Masaaki Takashige Terutaro Nakamura Yutaka Aikawa 《Ferroelectrics Letters Section》1983,44(11):313-318
The effect of isothermal annealing of amorphous PbTiO3 below the crystallization temperature, Tcrys has been investigated by Raman scattering measurements. The occurrence of the crystallization has been confirmed by the appearance of the soft mode. In the crystallized sample, the possibility of the occurrence of a crystalline phase, which is different from the tetragonal PbTiO3 of perovskite structure, has been pointed out. 相似文献
104.
Y Aikawa N Tanuma T Shin S Makino K Tanaka Y Matsumoto 《Canadian Metallurgical Quarterly》1998,89(1-2):35-42
Previous studies have shown that the calcitonin gene-related peptide (CGRP) immunoreactivity in the central nervous system (CNS) of adult rats is sexually dimorphic and regulated by sex steroid. In the present study, we used immunocytochemistry to investigate the sex difference in CGRP-immunoreactive (IR) neurons in rat dorsal root ganglia (DRG). The numbers of CGRP-IR neurons at the cervical, lumbar and sacral levels in the female rats were significantly lower than those of the male rats. We also found that the number of CGRP-IR neurons at the lumbar level was increased in ovariectomized (OVX) rats, but was decreased in estradiol (E2)-treated rats (OVX+E2). A large number of estrogen receptor (ER)-IR neurons at the lumbar level were found in the female rats, and its number was greater than that in the male rats. We also investigated the change in the number of ER-IR neurons of OVX rats after estrogen treatment. The number of ER-IR neurons in the OVX+E2 rats was consistent with that of the intact female rats, but was significantly increased in the OVX rats. As shown by a double-labeling immunocytochemical method, over 80% of the CGRP-IR neurons at the lumbar level showed ER immunoreactivity in the female, OVX and OVX+E2 rats, compared to only about 46% in the male rats. These results indicate that there is a gender difference in CGRP expression in the rat DRG, and that this CGRP expression might be downregulated by estrogen (at least in part) through its receptor. 相似文献
105.
T Chiba Y Matsuzaki M Abei J Shoda T Aikawa N Tanaka T Osuga 《Canadian Metallurgical Quarterly》1996,31(4):552-558
To elucidate the risk factors for hepatocellular carcinoma (HCC) in hepatitis C virus (HCV)-related liver cirrhosis (LC), we examined 204 cirrhotic patients negative for hepatitis B surface antigen and positive for HCV antibodies. The independent influence of various clinical characteristics in these patients was analyzed by multiple logistic regression, and the risk factors for HCC were identified. Multiple logistic regression analysis identified and ranked the following four risk factors: male sex (P < 0.001), habitual heavy drinking (P < 0.005), hepatitis B virus antibody positivity (anti-HBs and/or anti-HBc, P < 0.05), and age greater than 60 years (P < 0.05). The odds ratio of HCC was 4.20 (95% confidence interval; CI, 1.80-9.78) in male patients, 3.27 (95% CI, 1.46-7.30) in habitual heavy drinkers, 2.01 (95% CI, 1.01-3.99) in patients positive for hepatitis B virus antibodies, and 2.06 (95% CI, 1.00-4.23) in patients older than 60 years. The cumulative occurrence rates of HCC after blood transfusion were significantly higher in habitual heavy drinkers (4.8%, 49.4%, and 74.7% at 10, 20, and 30 years, respectively) than in non-drinkers (0%, 21.0%, and 23.3% at 10, 20, and 30 years, respectively, P < 0.0003). The mean interval for progression to LC after blood transfusion was significantly shorter in the habitual heavy drinkers than in the non-drinkers (22.4 +/- 4.4 years vs 28.4 +/- 3.9 years; P < 0.0003). This multivariate analysis revealed that habitual heavy drinking and hepatitis B virus antibody positivity are significant risk factors for HCC in HCV-related liver cirrhosis. 相似文献
106.
Y Zou I Komuro T Yamazaki R Aikawa S Kudoh I Shiojima Y Hiroi T Mizuno Y Yazaki 《Canadian Metallurgical Quarterly》1996,271(52):33592-33597
Angiotensin II (AngII) induces cardiac hypertrophy through activating a variety of protein kinases. In this study, to understand how cardiac hypertrophy develops, we examined AngII-evoked signal transduction pathways leading to the activation of extracellular signal-regulated protein kinases (ERKs), which are reportedly critical for the development of cardiac hypertrophy, in cultured cardiac myocytes isolated from neonatal rats. Inhibition of protein kinase C (PKC) with calphostin C or down-regulation of PKC by pretreatment with a phorbol ester for 24 h abolished AngII-induced activation of Raf-1 and ERKs, and addition of a phorbol ester conversely induced a marked increase in the activities of Raf-1 and ERKs. Pretreatment with two chemically and mechanistically dissimilar tyrosine kinase inhibitors, genistein and tyrphostin, did not attenuate AngII-induced activation of ERKs. In contrast, genistein strongly blocked insulin-induced ERK activation in cardiac myocytes. Although pretreatment with manumycin, a Ras farnesyltransferase inhibitor, or overexpression of a dominant-negative mutant of Ras inhibited insulin-induced ERK activation, neither affected AngII-induced activation of ERKs. Overexpression of a dominant-negative mutant of Raf-1 completely suppressed ERK2 activation by AngII, endothelin-1, and insulin. These results suggest that PKC and Raf-1, but not tyrosine kinases or Ras, are critical for AngII-induced activation of ERKs in cardiac myocytes. 相似文献
107.
Tokumitsu T. Hirano N. Yamasaki K. Yamaguchi C. Nishikawa K. Aikawa M. 《Solid-State Circuits, IEEE Journal of》1997,32(9):1334-1341
A novel three-dimensional (3-D) masterslice monolithic microwave integrated circuit (MMIC) is presented that significantly reduces turnaround time and cost for multifunction MMIC production. This MMIC incorporates an artificial ground metal for effective selection of master array elements on the wafer surface, resulting in various MMIC implementations on a master-arrayed footprint in association with thin polyimide and metal layers over it. Additionally, the 3-D miniature circuit components of less than 0.4 mm2 in size provide a very high integration level. To clearly show the advantages, a 20-GHz-band receiver MMIC was implemented on a master array with 6×3 array units including a total of 36 MESFETs in a 1.78×1.78 mm area. Details of the miniature circuit components and the design, closely related to the fabrication process, are also presented. The receiver MMIC exhibited a 19-dB conversion gain with an associated 6.5-dB noise figure from 17 to 24 GHz and an integration level four times higher than conventional planar MMICs. This technology promises about a 90% cost reduction for MMIC because it can be similarly applied to large-scale Si wafers with the aid of an artificial ground 相似文献
108.
Shingo Kurose Kunihito Yamamori Masaru Aikawa Ikuo Yoshihara 《Artificial Life and Robotics》2012,16(4):533-536
An island model is a typical implementation of genetic programming on parallel computers with distributed memory. The island
model has a migration facility that sends/receives some individuals in an island to/from another island to maintain diversity.
The island model requires synchronization to migrate same-generation individuals between islands, and this synchronization
causes an increase in computation time. This article proposes a new parallel genetic programming implementation based on the
island model with asynchronous migration. Most recent computers are equipped with one or more multi-core processors, and are
suitable for multi-threading. Therefore we employ a communication thread for migration between islands. The communication
thread on a processor communicates with the communication thread on another processor to migrate individuals at appropriate
intervals. Since the migration and other genetic operations can be independently processed on each core, and since we allow
the exchange of individuals of different generations, no synchronization is needed in our implementation. In addition, a fitness
calculation is also executed in parallel by the remaining cores. Experimental results show that the proposed method can reduce
the computation time to about 17% in serial GP by using 40 threads. 相似文献
109.
Chiba A. Furuichi R. Aikawa Y. Shimada K. Takamoto Y. Fukao T. 《Industry Applications, IEEE Transactions on》1997,33(4):919-924
A control method, which realizes stable operation in induction-type bearingless motors under loaded conditions, is proposed. Without a proposed control method, both the amplitude and the direction of radial force vary in accordance with loads. These variations result in interferences of radial force commands in two perpendicular axes. The interferences become a serious problem as a load increases. A shaft finally touches down without proper compensation. It is found that a decoupling of the radial force commands can be realized with compensation of both amplitude and phase angle of four-pole motor current. A decoupling controller is proposed with the principle of a field-oriented controller of conventional induction motors. The results of experiments show the validity of compensations. It is shown that induction-type bearingless motors can be operated stably under loaded conditions, as well as transient conditions 相似文献
110.
A reconfigurable antenna is attractive for advanced wireless communication systems in the future. A novel polarisation switchable slot-ring array antenna as a kind of reconfigurable antenna is proposed. To realise the orthogonal polarisation switchable antenna, four switching diodes are mounted on a slot-ring antenna element. The both-sided MIC technology is effectively employed in forming this slot-ring array antenna. The Ansoft HFSS is used to simulate the antenna, and the characteristics of this functional antenna are discussed. The calculated results are compared with the measured ones, and these results agree well. Consequently, a readability of the polarisation switchable antenna is successfully verified. 相似文献