A detection method of CryIAc protein for identifying genetically modified rice using the lateral flow strip assay

We examined the lateral flow strip assay for identifying unauthorized genetically modified (GM) rice. The GM rice expresses the Bacillus thuringiensis (Bt) toxin, CryIAc protein, which confers tolerance to insects. The recombinant CryIAc protein was prepared from the inclusion bodies of an E. coli. strain into which the CryIAc gene had been inserted, using gel filtration chromatography. The lateral flow strip assay for the identification of GM cotton which also expresses CryIAc protein, was applied to unpolished rice and polished rice spiked with recombinant CryIAc protein. The spiked recombinant CryIAc protein was clearly detected at the level of 0.012 microg/g in both the unpolished and polished rice. After loading of the extract on the strip, a 60 -minute stand time is necessary to clearly detect CryIAc protein. The detection limit was approximately 12 ng CryIAc protein per gram of rice. These results suggest that the lateral flow strip assay for GM cotton can be used to detect CryIAc protein expressed in GM rice.     

Effect of inserting a buffer layer on the characteristics of transparent conducting impurity-doped ZnO thin films prepared by dc magnetron sputtering

In transparent conducting impurity-doped ZnO thin films prepared on glass substrates by a dc magnetron sputtering (dc-MS) deposition, the obtainable lowest resistivity and the spatial resistivity distribution on the substrate surface were improved by a newly developed MS deposition method. The decrease of obtainable lowest resistivity as well as the improvement of spatial resistivity distribution on the substrate surface in Al- or Ga-doped ZnO (AZO or GZO) thin films were successfully achieved by inserting a very thin buffer layer, prepared using the same MS apparatus with the same target, between the thin film and the glass substrate. The deposition of the buffer layer required a more strongly oxidized target surface than possible to attain during a conventional dc-MS deposition. The optimal thickness of the buffer layer was found to be about 10 nm for both GZO and AZO thin films. The resistivity decrease is mainly attributed to an increase of Hall mobility rather than carrier concentration, resulting from an improvement of crystallinity coming from insertion of the buffer layer. Resistivities of 3 × 10^− 4 and 4 × 10^− 4Ω cm were obtained in 100 nm-thick-GZO and AZO thin films, respectively, incorporating a 10 nm-thick-buffer layer prepared at a substrate temperature around 200 °C.     

Feasibility of nonlinear Raman lidar based on stimulated Raman gain spectroscopy without a tunable laser

A novel technique of lidar for atmospheric gas detection by use of stimulated Raman gain spectroscopy without any tunable laser is proposed. Detection sensitivity and detectable range are estimated on the basis of the lidar equation for CO2, CH4, and H2 in the atmosphere. The feasibility study clearly shows that the technique has a potential for application to lidar and that, in addition, the construction of the system is simpler than those of traditional differential absorption lidars.     

SMEM algorithm is not fully compatible with maximum-likelihood framework

The expectation-maximization (EM) algorithm with split-and-merge operations (SMEM algorithm) proposed by Ueda, Nakano, Ghahramani, and Hinton (2000) is a nonlocal searching method, applicable to mixture models, for relaxing the local optimum property of the EM algorithm. In this article, we point out that the SMEM algorithm uses the acceptance-rejection evaluation method, which may pick up a distribution with smaller likelihood, and demonstrate that an increase in likelihood can then be guaranteed only by comparing log likelihoods.     

Antitumor vaccination effect of dendritic cells can be augmented by locally utilizing Th1-type cytokines from OK432-reactive CD4+ T cells

Tissue processed at a constant temperature of 45 C including the use of paraffin wax with a melting point of 45 C displays staining characteristics that are sometimes reversed from those associated with the more usual processing schedules and wax with a melting point of 58-60 C. Staining with acid dyes, particularly in trichrome methods, are most susceptible to these changes. We suggest that this is directly related to dye molecular size and to differences in the tissue structure resulting from the heat to which the tissues were exposed.     

Characteristics of high-energy neutrons estimated using the radioactive spallation products of Au at the 500-MeV neutron irradiation facility of KENS

We carried out a shielding experiment of high-energy neutrons, generated from a tungsten target bombarded with primary 500-MeV protons at KENS, which penetrated through a concrete shield in the zero-degree direction. We propose a new method to evaluate the spectra of high-energy neutrons ranging from 8 to 500 MeV. Au foils were set in a concrete shield, and the reaction rates for 13 radionuclides produced by the spallation reactions on the Au targets were measured by radiochemical techniques. The experimental results were compared with those obtained by the MARS14 Monte-Carlo code. A good agreement (between them) was found for energies beyond 100 MeV. The profile of the neutron spectrum, ranging from 8 to 500 MeV, does not depend on the thickness of the concrete shield.     

A new method for separating HFC-134a from gas mixtures using clathrate hydrate formation

A new separation method using gas hydrate formation is proposed for separating HFC-134a from gas mixtures containing N2 and HFC-134a. The feasibility of this separation method was investigated from various points of view. First, to determine the mixed hydrate stability region, three-phase equilibria of hydrate (H), liquid water (Lw), and vapor (V) for HFC-134a + N2 + water mixtures with various HFC-134a vapor compositions were closely examined in the temperature and pressure ranges of 275-285 K and 0.1-2.7 MPa, respectively. Second, the compositions of the hydrate and vapor phases at a three-phase equilibrium state were analyzed for identical mixtures at 278.15 and 282.15 K to confirm the actual separation efficiency. Third, kinetic experiments were performed to monitor the composition change behavior of the vapor phase and to determine the time required for an equilibrium state to be reached. Furthermore, X-ray diffraction confirmed that the mixed HFC-134a + N2 hydrates were structure II. Through an overall investigation of the experimental results, it was verified that more than 99 mol % HFC-134a could be obtained from gas mixtures after hydrate formation and subsequent dissociation processes. Separation of HFC-134a using hydrate formation can be carried out at mild temperature and low-pressure ranges. No additive is needed to lower the hydrate formation pressure.     

Effect of a traditional Chinese medicine, Bu-zhong-yi-qi-tang on the protection against an oral infection with Listeria monocytogenes

Gene therapy for vascular disease is in the beginning stages. Each year investigators are increasing our understanding of the molecular and cellular biology of vascular disease and its complications. Our genes exert exquisite control over the expressed molecular pattern that results in biological function and pathology. Gene transfer techniques can be used to affect the pattern of gene expression. Gene therapy is a powerful tool that will allow specific manipulation of the genetic cascade that determines biological function. Gene transfer techniques should help to define the molecular mechanisms involved in vascular pathology, such as atherosclerosis and its complications. Currently, gene therapy has only reached clinical trials, but this new technology will likely play a major role in our treatment of vascular problems in the future. An understanding of the significance of this new technology is important for both health care providers and patients.     

Microstructure, thermal properties and hardness of the CeMO3 (M=Al,Ga) synthesized by arc-melting method

Synthesis of CeMO₃ (M=Al,Ga) was attempted by the arc-melting method. In the case of M=Al, the arc-melted sample consisted typically of three regions CeAlO₃, CeO₂ and eutectic, mainly Al₂O₃. A similar texture was also observed for the case of M=Ga. Both CeAlO₃ and CeGaO₃ are crystallized in a tetragonal system (space group:P4/mmm). Lattice parameters of the tetragonal cell for CeAlO₃ and CeGaO₃ are a=0.37669(9) nm, c=0.37967(7) nm and a=0.3873(1) nm, c=0.3880(1) nm, respectively. According to thermal analysis, decomposition of 2CeMO₃+1/2O₂→2CeO₂+M₂O₃ occurs for CeAlO₃ and CeGaO₃ at about 1275°C and 833°C, respectively. The TG curve shows that the onset-temperature of oxidation for CeAlO₃ is at 1170°C and for CeGaO₃ is at 720°C. After TG-DTA measurement, both compounds are completely decomposed to starting materials. The value of the micro-Vickers hardness for the CeAlO₃ and CeGaO₃ is 9.0 (±0.2) GPa and 7.2 (±0.4) GPa, respectively.