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51.
In order to observe intracellular structures by scanning electron microscopy, excess cytoplasmic matrix must be removed from the fractured surface of cells. Previously we reported an Osmium-DMSO-Osmium method devised for this purpose. This method is very effective in revealing intracellular structures, but requires osmium tetroxide for initial fixation with some consequent disadvantages. In the present study, a revised Osmium-DMSO-Osmium method is reported, in which an aldehyde mixture is used as the initial fixative instead of osmium tetroxide. As fixation is carried out by perfusion in this revised method, better preservation of fine structures is achieved than by the original method, especially in the central nervous tissue which tends to suffer from post-mortem degeneration. Moreover this method can be applied to cytochemical studies of intracellular structures with a scanning electron microscope (SEM). In this study, acid phosphatase of lysosomes is demonstrated in a coloured SEM micrograph. 相似文献
52.
研究了粉末冶金机械零件使用的烧结材料的杨氏模量、切变模量及泊松比与孔隙度的关系.制备了三种钢粉,在不同条件下进行了压制、烧结与热处理.孔隙度对杨氏模量、切变模量及泊松比的影响最大.特别是泊松比还受孔隙形状的影响,而孔隙形状随粉末类型、烧结温度与烧结气氛及热处理条件而变化.对于大部分实际应用的粉末组成来说,烧结气氛对经烧结和热处理后材料的泊松比影响不大.对于部分预合金化粉末(Fe4%Ni1.5%Cu0.5%Mo)+0.8%石墨,只有在孔隙度低于20%时,烧结温度对泊松比与孔隙度的关系才有影响.在这种情况下.提出了烧结与热处理试样泊松比与孔隙度关系的较简单近似方程:在烧结温度1 423K下,v=0.300-0.266P+0.579P2;在烧结温度1 523K下,v=0.304-0.264P+0.548P2. 相似文献
53.
Hiroo Hasebe Hiromichi Ryuto Nobuhisa Fukunishi Akira Goto Masayuki Kase Yasushige Yano 《Nuclear instruments & methods in physics research. Section A, Accelerators, spectrometers, detectors and associated equipment》2008,590(1-3):13
The problem of handling the fragile carbon foils (mounting on the frame, placing in the stripper changer) that easily break when self-supporting has been solved by coating carbon foils with poly-monochloro-para-xylylene. It was found that the polymer-coating method could also be used to produce carbon foils thicker than 100 μg/cm2 by alternated deposition of carbon and poly-monochloro-para-xylylene layers. Carbon foil of 500 μg/cm2 thick and 10 cm in diameter was produced by this method and mounted to a foil holder. Results of lifetime measurement for singly coated foils are also presented. 相似文献
54.
Yukito Naitoh Akira Otomo Hideki Miki Isao Aoki Shiyosi Yokoyama 《Thin solid films》2008,516(9):2371-2375
We studied energy transfer between rhodamine B molecules centered in each corn-shaped dendrons that forms self-assembled monolayer (SAM) film on an Au substrate. A SAM film using dendron moiety as a spacer can change the distance between rhodamines depending on the size, so that the energy transfer from an initially photo-excited rhodamine to its surrounding molecules can be controlled. The absorption spectrum of a SAM film for each generation of dendron was measured to ascertain a linear correlation between the generation and the distance. Picosecond time-resolved fluorescence spectra showed clear differences in the fluorescence decay dynamics between third-generation (G3) and fourth-generation (G4) dendron SAM films. In addition, we found that “dry” or “wet” dendron SAM considerably influenced fluorescence behavior. As a result, the use of “wet” G4 SAM is suitable in preserving photo-excitation energy. This is because it gave almost equal dynamics to G4 dendron in the dilute solution and prevented deactivation by energy dissipation. 相似文献
55.
This study describes a new method of experimental design to obtain a response surface of buckling load of laminated composites. Many evaluations for genetic algorithms for stacking sequence optimizations require high computational cost. That evaluation cost can be reduced by an approximation using a response surface. For a response surface for stacking sequence optimizations, lamination parameters are adopted as variables of the approximation function of the entire design space instead of ply angles for each ply. This study presents, proposes and investigates a new method of experimental design in detail. For most analytical tools, stacking sequences is demand as input data and lamination parameters cannot be applied directly to the tools. Therefore, the present study proposes and applies a new D-optimal set of laminates to the stacking sequence optimizations of the problem of maximization of buckling load of a composite cylinder. The new experimental design is a set of stacking sequences selected from candidate stacks using D-optimality. Consequently, the D-optimal set of laminates is shown to be effective for design of experiments of response surfaces for maximization of the buckling load of composite structures. 相似文献
56.
Natacha Kalline de Oliveira Lucyene Miguita Tais Helena Costa Salles Marcos Akira d’Ávila Márcia Martins Marques Maria Cristina Zindel Deboni 《Journal of Materials Science》2018,53(23):15757-15768
The aim of this study was to analyze the influence of nanoporous structure of polymeric biomaterials on the in vitro osteogenic induction of human stem cells. An electronic search in three databases (MEDLINE, SCOPUS, and Web of Science) was performed for articles that were published before May 2018. In vitro studies were included if they met the following criteria: (1) the use of polymeric scaffolds (natural or synthetic); (2) the co-culture of human stem cells with the scaffold; and (3) cell viability, proliferation, and osteogenic differentiation assays. The main characteristics of the published studies were summarized, and a quality assessment tool was used to analyze methodological features. Eighty-eight potential articles were firstly retrieved. Thirteen were eligible for qualitative analysis. Only three studies characterized cell stemness. Nanostructure of the scaffolds showed a significant influence on viability, proliferation, and osteogenic differentiation of human stem cells. Combination of porosity between 72 and 93% and a large range diameter between 50 and 224 μm resulted in more remarkable cellular proliferation and differentiation. Porous polymeric scaffolds can be functionalized by stem cells leading to osteogenic induction. High standards of laboratory practice and accurate methodological reporting are essential for the credibility of the results. 相似文献
57.
In this paper, a decentralized proportional-derivative (PD) controller design for non-uniform motion of a Hamiltonian hybrid system is considered. A Hamiltonian hybrid system with the capability of producing a non-uniform motion is developed. The structural properties of the system are investigated by means of the theory of Hamiltonian systems. A relationship between the parameters of the system and the parameters of the proposed decentralized PD controller is shown to ensure local stability and tracking performance. Simulation results are included to show the obtained non-uniform motion. 相似文献
58.
A solid-particle erosive wear test by impinging silicon carbide (SiC) powders was carried out at room temperature over a range of median particle sizes of 425–600 μm, speed of 100 m/s and impact angle of 90° and assessed by wear measurements and scanning electron microscopy. Erosive wear behaviour was examined on newly fabricated nano-powder infiltration and transient eutectoid (NITE) SiC/SiC composites and two commercial composites by the chemical vapour infiltration (CVI) and NITE fabrication route. Microstructural observation was performed to examine the correlation between erosive wear behaviours and fabrication impurities. Conspicuous defects were observed in the prototype materials as the forms of porosity, fibre deformation, residual oxide, pyrolytic carbon (PyC) deformation, PyC cleavage, among others. Erosive wear behaviour was rather serious in the prototype of fabricated composites, which employ pre-SiC fibre and phenolic resin. Two dominant erosive wear mechanisms were observed: delamination of constituents, mainly caused by erosive crack propagation, and fragmentation and detachment of constituents, which usually resulted from erosive impact. A unit size of delamination was the most decisive factor affecting wear volume. The bonding strength of each constituent was mostly affected by various forms of porosities. Therefore, the fundamental cause and subsequent results must be carefully elucidated. The correlation of microstructural defect and wear behaviour was investigated with the aim of reducing dominant wear by improving fabrication conditions. The final product of the cost-effective composite had a 2.5-fold higher resistance than the commercial CVI composite. Consequently, by controlling fabrication impurities, we have been successful in developing and improving a new fabrication technique; consequently, the known defects are rarely observed in final product. A schematic wear model of erosive wear mechanisms is proposed for the newly fabricated SiC/SiC composites under particle erosion. 相似文献
59.
In this study, we prepared monodisperse spherical particles of a desired diameter using [(Fe0.5Co0.5)0.75B0.2Si0.05]96Nb4 alloy; the particles were prepared by using an atomization process developed by us. The particles have perfect sphericity and narrow size distribution along with a homogeneous composition. The phase transitions of particles from the fully glassy phase to the crystalline phase via mixed phase structures occurred as the particle diameter was increased; the particles produced in the fully glassy phase in an argon atmosphere had a diameter of less than 300 μm. This allowed the estimation of the intrinsic critical cooling rate for the particles with a fully glassy phase, Rc:Rc varied in the range of 700-900 K/s and depended only on the initial temperature of the alloy melt. 相似文献
60.
Koh Matsumoto Kazutada Ikenaga Jun Yamamoto Kazuki Naito Yoshiki Yano Akinori Ubukata Hiroki Tokunaga Tadanobu Arimura Katsuaki Cho Toshiya Tabuchi Akira Yamaguchi Yasuhiro Harada Yuzaburo Ban Kousuke Uchiyama 《半导体学报》2011,32(1):21-23
Growth rate has a direct impact on the productivity of nitride LED production.Atmospheric pressure growth of GaN with a growth rate as high as 10μm/h and also Al0.1Ga0.9N growth of 1μm/h by using 4 inch by 11 production scale MOVPE are described.XRD of(002) and(102) direction was 200 arcsec and 250 arcsec, respectively.Impact of the growth rate on productivity is discussed. 相似文献